Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6503 and GFF6504 overlap by 4 nucleotides GFF6504 and GFF6505 are separated by 4 nucleotides GFF6505 and GFF6506 are separated by 112 nucleotides
GFF6503 - no description, at 105 to 368
GFF6503
GFF6504 - no description, at 365 to 1,111
GFF6504
GFF6505 - no description, at 1,116 to 1,529
GFF6505
GFF6506 - DNA-binding protein HU-beta, at 1,642 to 1,953
GFF6506
Position (kb) 0
1
2 Strain fitness (log2 ratio) -2
-1
0
1
2 at 0.071 kb on + strand at 0.071 kb on + strand at 0.094 kb on - strand at 0.111 kb on + strand at 0.191 kb on - strand, within GFF6503 at 0.191 kb on - strand, within GFF6503 at 0.191 kb on - strand, within GFF6503 at 0.264 kb on + strand, within GFF6503 at 0.264 kb on + strand, within GFF6503 at 0.264 kb on + strand, within GFF6503 at 0.265 kb on - strand, within GFF6503 at 0.265 kb on - strand, within GFF6503 at 0.265 kb on - strand, within GFF6503 at 0.333 kb on + strand, within GFF6503 at 0.333 kb on + strand, within GFF6503 at 0.333 kb on + strand, within GFF6503 at 0.334 kb on - strand, within GFF6503 at 0.334 kb on - strand, within GFF6503 at 0.334 kb on - strand, within GFF6503 at 0.366 kb on + strand at 0.366 kb on + strand at 0.367 kb on - strand at 0.367 kb on - strand at 0.555 kb on + strand, within GFF6504 at 0.556 kb on - strand, within GFF6504 at 0.556 kb on - strand, within GFF6504 at 0.556 kb on - strand, within GFF6504 at 0.657 kb on + strand, within GFF6504 at 0.657 kb on + strand, within GFF6504 at 0.657 kb on + strand, within GFF6504 at 0.658 kb on - strand, within GFF6504 at 0.658 kb on - strand, within GFF6504 at 0.779 kb on - strand, within GFF6504 at 0.795 kb on + strand, within GFF6504 at 0.795 kb on + strand, within GFF6504 at 0.796 kb on - strand, within GFF6504 at 0.796 kb on - strand, within GFF6504 at 0.796 kb on - strand, within GFF6504 at 0.796 kb on - strand, within GFF6504 at 0.796 kb on - strand, within GFF6504 at 0.932 kb on + strand, within GFF6504 at 0.933 kb on - strand, within GFF6504 at 0.987 kb on - strand, within GFF6504 at 0.987 kb on - strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.988 kb on + strand, within GFF6504 at 0.989 kb on - strand, within GFF6504 at 0.989 kb on - strand, within GFF6504 at 0.989 kb on - strand, within GFF6504 at 0.989 kb on - strand, within GFF6504 at 0.989 kb on - strand, within GFF6504 at 0.989 kb on - strand, within GFF6504 at 0.994 kb on - strand, within GFF6504 at 1.045 kb on + strand at 1.046 kb on - strand at 1.099 kb on + strand at 1.339 kb on - strand, within GFF6505 at 1.339 kb on - strand, within GFF6505 at 1.582 kb on - strand at 1.596 kb on - strand at 1.613 kb on + strand at 1.643 kb on + strand at 1.644 kb on - strand at 1.644 kb on - strand at 1.644 kb on - strand at 1.906 kb on + strand, within GFF6506 at 1.906 kb on + strand, within GFF6506 at 1.906 kb on + strand, within GFF6506 at 1.906 kb on + strand, within GFF6506 at 1.907 kb on - strand, within GFF6506 at 1.907 kb on - strand, within GFF6506 at 1.972 kb on + strand at 1.973 kb on - strand at 1.990 kb on + strand at 2.003 kb on + strand at 2.003 kb on + strand at 2.004 kb on - strand at 2.004 kb on - strand at 2.004 kb on - strand at 2.105 kb on + strand at 2.105 kb on + strand at 2.106 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 71 + +0.6 71 + -1.0 94 - -0.7 111 + -0.5 191 - GFF6503 0.33 -2.6 191 - GFF6503 0.33 -1.4 191 - GFF6503 0.33 -0.2 264 + GFF6503 0.60 -0.4 264 + GFF6503 0.60 -0.7 264 + GFF6503 0.60 -0.9 265 - GFF6503 0.61 -0.3 265 - GFF6503 0.61 -0.6 265 - GFF6503 0.61 -0.0 333 + GFF6503 0.86 -0.2 333 + GFF6503 0.86 -0.8 333 + GFF6503 0.86 -0.1 334 - GFF6503 0.87 +1.1 334 - GFF6503 0.87 +2.0 334 - GFF6503 0.87 -0.4 366 + -0.2 366 + -0.6 367 - +0.7 367 - -0.5 555 + GFF6504 0.25 +0.4 556 - GFF6504 0.26 -1.7 556 - GFF6504 0.26 +0.1 556 - GFF6504 0.26 -1.2 657 + GFF6504 0.39 -0.1 657 + GFF6504 0.39 +1.2 657 + GFF6504 0.39 +1.2 658 - GFF6504 0.39 +0.9 658 - GFF6504 0.39 -0.0 779 - GFF6504 0.55 -0.7 795 + GFF6504 0.58 -0.1 795 + GFF6504 0.58 +1.8 796 - GFF6504 0.58 -0.8 796 - GFF6504 0.58 +0.0 796 - GFF6504 0.58 -0.4 796 - GFF6504 0.58 -0.8 796 - GFF6504 0.58 -1.0 932 + GFF6504 0.76 +1.4 933 - GFF6504 0.76 -0.6 987 - GFF6504 0.83 -0.0 987 - GFF6504 0.83 -0.3 988 + GFF6504 0.83 +0.3 988 + GFF6504 0.83 +0.3 988 + GFF6504 0.83 -0.0 988 + GFF6504 0.83 +0.5 988 + GFF6504 0.83 -0.3 988 + GFF6504 0.83 +0.0 988 + GFF6504 0.83 +0.1 989 - GFF6504 0.84 -0.5 989 - GFF6504 0.84 +0.4 989 - GFF6504 0.84 +2.7 989 - GFF6504 0.84 +0.9 989 - GFF6504 0.84 -0.3 989 - GFF6504 0.84 -0.3 994 - GFF6504 0.84 +0.3 1,045 + -0.4 1,046 - -0.1 1,099 + +0.2 1,339 - GFF6505 0.54 +0.3 1,339 - GFF6505 0.54 +0.6 1,582 - +0.2 1,596 - +2.4 1,613 + +0.0 1,643 + -0.0 1,644 - -0.8 1,644 - -0.9 1,644 - +0.6 1,906 + GFF6506 0.85 -0.4 1,906 + GFF6506 0.85 -0.7 1,906 + GFF6506 0.85 +0.3 1,906 + GFF6506 0.85 +0.2 1,907 - GFF6506 0.85 -0.8 1,907 - GFF6506 0.85 -0.3 1,972 + +1.0 1,973 - -1.9 1,990 + +0.2 2,003 + -0.4 2,003 + -0.0 2,004 - +0.2 2,004 - -0.5 2,004 - -0.2 2,105 + -0.3 2,105 + -0.2 2,106 - -0.0
Or see this region's nucleotide sequence