Strain Fitness in Variovorax sp. SCN45 around GFF6490

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
66,856	+		GFF6488	0.50	-0.5
66,856	+		GFF6488	0.50	-0.7
66,856	+		GFF6488	0.50	+0.8
66,857	-		GFF6488	0.51	-0.9
66,857	-		GFF6488	0.51	-0.1
66,870	-		GFF6488	0.62	+0.1
66,915	+				-1.2
66,915	+				-0.2
66,915	+				+0.8
66,915	+				-0.6
66,916	-				-1.4
66,916	-				-0.3
66,916	-				-1.1
66,916	-				-0.6
66,973	+				+0.1
67,113	+				-2.4
67,113	+				-1.6
67,113	+				-0.2
67,113	+				-0.4
67,113	+				-0.5
67,113	+				+1.0
67,113	+				+0.6
67,113	-				-0.8
67,114	-				-1.4
67,114	-				+0.7
67,114	-				-1.2
67,114	-				-0.4
67,114	-				-0.5
67,128	+				+0.0
67,155	+				-0.4
67,155	+				+1.0
67,155	+				+1.0
67,155	+				-1.7
67,155	+				-0.2
67,156	-				-1.3
67,156	-				+0.3
67,156	-				-1.4
67,156	-				-0.1
67,156	-				+1.4
67,156	-				+0.4
67,203	+				+0.3
67,203	+				-0.6
67,203	+				-2.1
67,204	-				-0.2
67,204	-				+0.7
67,447	+				-0.6
67,448	-				-1.0
67,448	-				+0.4
67,471	+		GFF6489	0.13	-0.2
67,471	+		GFF6489	0.13	-0.3
67,471	+		GFF6489	0.13	-0.2
67,471	+		GFF6489	0.13	-0.4
67,471	+		GFF6489	0.13	-0.9
67,472	-		GFF6489	0.13	-0.0
67,531	+		GFF6489	0.27	-1.5
67,531	+		GFF6489	0.27	+1.0
67,531	+		GFF6489	0.27	-2.0
67,531	+		GFF6489	0.27	-1.5
67,531	+		GFF6489	0.27	-0.8
67,531	+		GFF6489	0.27	-0.2
67,531	+		GFF6489	0.27	-0.7
67,531	+		GFF6489	0.27	-1.0
67,532	-		GFF6489	0.27	+0.9
67,532	-		GFF6489	0.27	-0.5
67,532	-		GFF6489	0.27	-0.6
67,532	-		GFF6489	0.27	-0.4
67,532	-		GFF6489	0.27	-0.1
67,532	-		GFF6489	0.27	-1.0
67,532	-		GFF6489	0.27	-0.2
67,532	-		GFF6489	0.27	+1.1
67,532	-		GFF6489	0.27	+0.6
67,532	-		GFF6489	0.27	-0.9
67,741	+		GFF6489	0.74	+0.2
67,742	-		GFF6489	0.74	-0.5
67,742	-		GFF6489	0.74	-0.0
67,764	-		GFF6489	0.79	+0.1
67,959	+		GFF6490	0.15	-1.8
67,959	+		GFF6490	0.15	-0.3
67,960	-		GFF6490	0.15	+0.1
67,960	-		GFF6490	0.15	-0.3
67,960	-		GFF6490	0.15	-0.1
67,983	+		GFF6490	0.19	+0.5
68,091	+		GFF6490	0.34	-0.9
68,091	+		GFF6490	0.34	+0.1
68,091	+		GFF6490	0.34	-0.5
68,091	+		GFF6490	0.34	-0.9
68,091	+		GFF6490	0.34	-1.0
68,091	+		GFF6490	0.34	-0.3
68,091	+		GFF6490	0.34	-0.2
68,092	-		GFF6490	0.34	-1.2
68,092	-		GFF6490	0.34	-0.6
68,143	-		GFF6490	0.42	-0.4
68,143	-		GFF6490	0.42	-1.8
68,143	-		GFF6490	0.42	+0.3
68,143	-		GFF6490	0.42	-0.1
68,226	+		GFF6490	0.54	+1.4
68,226	+		GFF6490	0.54	-0.2
68,226	+		GFF6490	0.54	+0.2
68,226	+		GFF6490	0.54	+0.4
68,227	-		GFF6490	0.54	-1.3
68,227	-		GFF6490	0.54	+0.9
68,289	+		GFF6490	0.63	+2.2
68,289	+		GFF6490	0.63	-0.5
68,289	+		GFF6490	0.63	-0.3
68,289	+		GFF6490	0.63	-0.9
68,290	-		GFF6490	0.63	-0.7
68,290	-		GFF6490	0.63	-0.2
68,290	-		GFF6490	0.63	-0.7
68,290	-		GFF6490	0.63	-0.5
68,290	-		GFF6490	0.63	+1.4
68,474	+		GFF6490	0.90	-0.6
68,666	+				+0.1
68,667	-				-0.7
68,813	+		GFF6491	0.27	+1.0
69,005	+		GFF6491	0.55	-0.8
69,005	+		GFF6491	0.55	-0.5
69,005	+		GFF6491	0.55	-0.0
69,005	+		GFF6491	0.55	-0.8
69,006	-		GFF6491	0.55	-0.2
69,006	-		GFF6491	0.55	-0.2
69,006	-		GFF6491	0.55	+1.2
69,006	-		GFF6491	0.55	+0.1
69,006	-		GFF6491	0.55	-0.2
69,006	-		GFF6491	0.55	-0.6
69,006	-		GFF6491	0.55	-1.2