Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6399 and GFF6400 are separated by 40 nucleotides GFF6400 and GFF6401 are separated by 11 nucleotides
GFF6399 - 5-methyltetrahydrofolate--homocysteine methyltransferase (EC 2.1.1.13), N-terminal domain, at 73,425 to 74,477
GFF6399
GFF6400 - N-formylglutamate deformylase (EC 3.5.1.68), at 74,518 to 75,342
GFF6400
GFF6401 - Formiminoglutamic iminohydrolase (EC 3.5.3.13), at 75,354 to 76,736
GFF6401
Position (kb)
74
75
76 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.666 kb on + strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.667 kb on - strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.702 kb on + strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.703 kb on - strand, within GFF6399 at 73.876 kb on + strand, within GFF6399 at 73.876 kb on + strand, within GFF6399 at 73.876 kb on + strand, within GFF6399 at 73.876 kb on + strand, within GFF6399 at 73.876 kb on + strand, within GFF6399 at 73.877 kb on - strand, within GFF6399 at 73.877 kb on - strand, within GFF6399 at 73.877 kb on - strand, within GFF6399 at 73.877 kb on - strand, within GFF6399 at 73.877 kb on - strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.981 kb on + strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.982 kb on - strand, within GFF6399 at 73.984 kb on + strand, within GFF6399 at 73.984 kb on + strand, within GFF6399 at 73.984 kb on + strand, within GFF6399 at 73.985 kb on - strand, within GFF6399 at 73.985 kb on - strand, within GFF6399 at 73.985 kb on - strand, within GFF6399 at 73.985 kb on - strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.185 kb on + strand, within GFF6399 at 74.186 kb on - strand, within GFF6399 at 74.186 kb on - strand, within GFF6399 at 74.186 kb on - strand, within GFF6399 at 74.186 kb on - strand, within GFF6399 at 74.186 kb on - strand, within GFF6399 at 74.186 kb on - strand, within GFF6399 at 74.254 kb on + strand, within GFF6399 at 74.255 kb on - strand, within GFF6399 at 74.255 kb on - strand, within GFF6399 at 74.255 kb on - strand, within GFF6399 at 74.255 kb on - strand, within GFF6399 at 74.255 kb on - strand, within GFF6399 at 74.356 kb on + strand, within GFF6399 at 74.459 kb on + strand at 74.460 kb on - strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.461 kb on + strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.462 kb on - strand at 74.477 kb on + strand at 74.477 kb on + strand at 74.478 kb on - strand at 74.478 kb on - strand at 74.490 kb on + strand at 74.490 kb on + strand at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.636 kb on + strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.637 kb on - strand, within GFF6400 at 74.694 kb on - strand, within GFF6400 at 74.694 kb on - strand, within GFF6400 at 74.708 kb on + strand, within GFF6400 at 74.708 kb on + strand, within GFF6400 at 74.708 kb on + strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.709 kb on - strand, within GFF6400 at 74.744 kb on + strand, within GFF6400 at 74.744 kb on + strand, within GFF6400 at 74.951 kb on + strand, within GFF6400 at 74.951 kb on + strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 74.952 kb on - strand, within GFF6400 at 75.020 kb on + strand, within GFF6400 at 75.020 kb on + strand, within GFF6400 at 75.020 kb on + strand, within GFF6400 at 75.021 kb on - strand, within GFF6400 at 75.021 kb on - strand, within GFF6400 at 75.081 kb on - strand, within GFF6400 at 75.081 kb on - strand, within GFF6400 at 75.081 kb on - strand, within GFF6400 at 75.120 kb on + strand, within GFF6400 at 75.122 kb on + strand, within GFF6400 at 75.122 kb on + strand, within GFF6400 at 75.122 kb on + strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.123 kb on - strand, within GFF6400 at 75.176 kb on + strand, within GFF6400 at 75.176 kb on + strand, within GFF6400 at 75.177 kb on - strand, within GFF6400 at 75.177 kb on - strand, within GFF6400 at 75.177 kb on - strand, within GFF6400 at 75.509 kb on + strand, within GFF6401 at 75.509 kb on + strand, within GFF6401 at 75.510 kb on - strand, within GFF6401 at 75.510 kb on - strand, within GFF6401 at 75.510 kb on - strand, within GFF6401 at 75.716 kb on - strand, within GFF6401 at 75.716 kb on - strand, within GFF6401 at 75.716 kb on - strand, within GFF6401 at 75.883 kb on + strand, within GFF6401 at 75.883 kb on + strand, within GFF6401 at 75.884 kb on - strand, within GFF6401 at 75.884 kb on - strand, within GFF6401 at 75.890 kb on - strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.255 kb on + strand, within GFF6401 at 76.339 kb on + strand, within GFF6401
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 73,666 + GFF6399 0.23 -0.2 73,666 + GFF6399 0.23 +1.4 73,666 + GFF6399 0.23 -0.2 73,666 + GFF6399 0.23 -0.4 73,666 + GFF6399 0.23 -0.4 73,666 + GFF6399 0.23 -0.6 73,666 + GFF6399 0.23 +0.4 73,666 + GFF6399 0.23 +0.4 73,666 + GFF6399 0.23 -0.1 73,666 + GFF6399 0.23 -2.2 73,666 + GFF6399 0.23 -0.5 73,666 + GFF6399 0.23 -1.5 73,666 + GFF6399 0.23 +0.2 73,666 + GFF6399 0.23 -0.7 73,666 + GFF6399 0.23 -2.3 73,666 + GFF6399 0.23 -1.6 73,666 + GFF6399 0.23 -0.1 73,666 + GFF6399 0.23 -0.8 73,666 + GFF6399 0.23 -0.5 73,667 - GFF6399 0.23 +0.2 73,667 - GFF6399 0.23 +0.2 73,667 - GFF6399 0.23 -1.0 73,667 - GFF6399 0.23 -0.2 73,667 - GFF6399 0.23 -0.2 73,667 - GFF6399 0.23 +1.6 73,667 - GFF6399 0.23 -0.5 73,667 - GFF6399 0.23 +0.6 73,667 - GFF6399 0.23 -0.8 73,667 - GFF6399 0.23 +0.4 73,667 - GFF6399 0.23 -0.1 73,667 - GFF6399 0.23 -0.1 73,667 - GFF6399 0.23 -0.8 73,667 - GFF6399 0.23 +2.1 73,667 - GFF6399 0.23 -0.7 73,667 - GFF6399 0.23 +0.8 73,667 - GFF6399 0.23 -0.0 73,667 - GFF6399 0.23 -1.4 73,667 - GFF6399 0.23 -0.5 73,667 - GFF6399 0.23 -0.5 73,667 - GFF6399 0.23 +0.4 73,667 - GFF6399 0.23 +0.4 73,667 - GFF6399 0.23 -0.3 73,667 - GFF6399 0.23 -0.5 73,667 - GFF6399 0.23 -0.4 73,667 - GFF6399 0.23 -0.2 73,667 - GFF6399 0.23 -0.2 73,667 - GFF6399 0.23 +0.9 73,667 - GFF6399 0.23 -0.4 73,667 - GFF6399 0.23 -0.8 73,702 + GFF6399 0.26 -0.5 73,702 + GFF6399 0.26 -0.2 73,702 + GFF6399 0.26 -0.8 73,702 + GFF6399 0.26 -1.4 73,702 + GFF6399 0.26 -0.2 73,702 + GFF6399 0.26 -0.8 73,702 + GFF6399 0.26 +0.2 73,702 + GFF6399 0.26 +0.8 73,703 - GFF6399 0.26 +1.2 73,703 - GFF6399 0.26 -0.6 73,703 - GFF6399 0.26 -0.7 73,703 - GFF6399 0.26 -0.4 73,703 - GFF6399 0.26 -1.1 73,703 - GFF6399 0.26 +2.3 73,703 - GFF6399 0.26 +1.4 73,703 - GFF6399 0.26 +1.2 73,876 + GFF6399 0.43 +0.1 73,876 + GFF6399 0.43 -0.2 73,876 + GFF6399 0.43 -0.2 73,876 + GFF6399 0.43 -0.3 73,876 + GFF6399 0.43 +1.0 73,877 - GFF6399 0.43 +0.8 73,877 - GFF6399 0.43 -0.1 73,877 - GFF6399 0.43 -0.1 73,877 - GFF6399 0.43 -1.4 73,877 - GFF6399 0.43 +0.8 73,981 + GFF6399 0.53 -0.6 73,981 + GFF6399 0.53 +0.8 73,981 + GFF6399 0.53 -1.6 73,981 + GFF6399 0.53 -0.6 73,981 + GFF6399 0.53 -0.4 73,981 + GFF6399 0.53 -0.2 73,981 + GFF6399 0.53 +0.0 73,981 + GFF6399 0.53 -0.1 73,981 + GFF6399 0.53 -0.5 73,981 + GFF6399 0.53 -0.2 73,982 - GFF6399 0.53 +0.8 73,982 - GFF6399 0.53 -2.2 73,982 - GFF6399 0.53 +0.3 73,982 - GFF6399 0.53 +0.0 73,982 - GFF6399 0.53 -0.8 73,982 - GFF6399 0.53 +0.3 73,982 - GFF6399 0.53 -1.3 73,982 - GFF6399 0.53 +0.8 73,982 - GFF6399 0.53 +0.2 73,984 + GFF6399 0.53 +0.0 73,984 + GFF6399 0.53 +0.0 73,984 + GFF6399 0.53 +0.3 73,985 - GFF6399 0.53 +0.1 73,985 - GFF6399 0.53 +0.1 73,985 - GFF6399 0.53 +0.0 73,985 - GFF6399 0.53 +0.3 74,185 + GFF6399 0.72 -0.3 74,185 + GFF6399 0.72 +1.2 74,185 + GFF6399 0.72 -0.5 74,185 + GFF6399 0.72 +0.1 74,185 + GFF6399 0.72 -1.8 74,185 + GFF6399 0.72 +0.4 74,185 + GFF6399 0.72 -0.6 74,185 + GFF6399 0.72 +0.3 74,185 + GFF6399 0.72 +1.3 74,186 - GFF6399 0.72 -0.3 74,186 - GFF6399 0.72 -0.1 74,186 - GFF6399 0.72 -0.3 74,186 - GFF6399 0.72 +1.1 74,186 - GFF6399 0.72 -0.4 74,186 - GFF6399 0.72 -0.4 74,254 + GFF6399 0.79 -0.6 74,255 - GFF6399 0.79 -0.2 74,255 - GFF6399 0.79 -1.1 74,255 - GFF6399 0.79 -0.3 74,255 - GFF6399 0.79 +0.3 74,255 - GFF6399 0.79 -0.2 74,356 + GFF6399 0.88 -0.2 74,459 + -0.6 74,460 - +0.9 74,461 + -0.4 74,461 + +0.9 74,461 + -0.2 74,461 + -1.2 74,461 + +2.0 74,461 + +0.1 74,461 + +0.4 74,461 + -0.8 74,461 + +1.2 74,461 + +0.9 74,461 + -1.3 74,461 + -0.3 74,461 + -1.4 74,461 + +1.0 74,461 + +0.2 74,461 + -0.0 74,462 - -0.4 74,462 - +0.1 74,462 - -0.5 74,462 - +0.8 74,462 - -0.4 74,462 - -2.2 74,462 - +0.4 74,462 - -0.1 74,462 - +0.8 74,462 - -0.6 74,462 - -1.2 74,462 - +0.3 74,462 - +0.6 74,462 - -0.3 74,462 - -0.3 74,462 - -0.4 74,462 - -1.3 74,462 - -0.8 74,462 - +0.4 74,477 + -0.1 74,477 + +0.3 74,478 - +0.6 74,478 - +0.1 74,490 + +0.2 74,490 + -1.6 74,636 + GFF6400 0.14 +1.8 74,636 + GFF6400 0.14 +0.1 74,636 + GFF6400 0.14 -1.7 74,636 + GFF6400 0.14 -1.6 74,636 + GFF6400 0.14 +0.8 74,636 + GFF6400 0.14 +0.1 74,636 + GFF6400 0.14 -0.6 74,636 + GFF6400 0.14 -0.6 74,636 + GFF6400 0.14 -0.1 74,636 + GFF6400 0.14 -0.2 74,636 + GFF6400 0.14 -0.7 74,637 - GFF6400 0.14 -0.4 74,637 - GFF6400 0.14 -0.9 74,637 - GFF6400 0.14 +1.8 74,637 - GFF6400 0.14 +0.8 74,637 - GFF6400 0.14 -0.4 74,637 - GFF6400 0.14 +0.8 74,637 - GFF6400 0.14 +0.4 74,637 - GFF6400 0.14 +0.3 74,637 - GFF6400 0.14 +0.1 74,637 - GFF6400 0.14 -0.7 74,637 - GFF6400 0.14 -0.9 74,637 - GFF6400 0.14 -0.6 74,694 - GFF6400 0.21 -0.6 74,694 - GFF6400 0.21 +0.1 74,708 + GFF6400 0.23 -0.7 74,708 + GFF6400 0.23 -0.6 74,708 + GFF6400 0.23 -0.4 74,709 - GFF6400 0.23 -0.5 74,709 - GFF6400 0.23 +0.6 74,709 - GFF6400 0.23 -0.5 74,709 - GFF6400 0.23 +0.5 74,744 + GFF6400 0.27 -2.5 74,744 + GFF6400 0.27 -0.1 74,951 + GFF6400 0.52 +0.7 74,951 + GFF6400 0.52 -0.1 74,952 - GFF6400 0.53 +0.5 74,952 - GFF6400 0.53 -0.6 74,952 - GFF6400 0.53 +0.2 74,952 - GFF6400 0.53 +0.0 75,020 + GFF6400 0.61 -0.6 75,020 + GFF6400 0.61 +0.0 75,020 + GFF6400 0.61 -0.6 75,021 - GFF6400 0.61 -0.1 75,021 - GFF6400 0.61 +0.4 75,081 - GFF6400 0.68 -0.2 75,081 - GFF6400 0.68 -0.7 75,081 - GFF6400 0.68 +0.5 75,120 + GFF6400 0.73 -1.2 75,122 + GFF6400 0.73 -0.2 75,122 + GFF6400 0.73 +0.2 75,122 + GFF6400 0.73 -1.8 75,123 - GFF6400 0.73 -2.6 75,123 - GFF6400 0.73 -2.2 75,123 - GFF6400 0.73 -0.6 75,123 - GFF6400 0.73 -1.2 75,176 + GFF6400 0.80 +0.2 75,176 + GFF6400 0.80 -0.4 75,177 - GFF6400 0.80 -0.2 75,177 - GFF6400 0.80 -0.9 75,177 - GFF6400 0.80 -2.4 75,509 + GFF6401 0.11 -0.8 75,509 + GFF6401 0.11 -0.1 75,510 - GFF6401 0.11 -0.4 75,510 - GFF6401 0.11 -0.8 75,510 - GFF6401 0.11 -0.5 75,716 - GFF6401 0.26 -0.1 75,716 - GFF6401 0.26 +1.8 75,716 - GFF6401 0.26 -0.4 75,883 + GFF6401 0.38 +0.3 75,883 + GFF6401 0.38 -0.5 75,884 - GFF6401 0.38 +1.8 75,884 - GFF6401 0.38 -1.5 75,890 - GFF6401 0.39 -0.4 76,255 + GFF6401 0.65 -0.5 76,255 + GFF6401 0.65 -0.7 76,255 + GFF6401 0.65 -0.1 76,339 + GFF6401 0.71 +0.9
Or see this region's nucleotide sequence