Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6248 and GFF6249 overlap by 4 nucleotides GFF6249 and GFF6250 overlap by 4 nucleotides
GFF6248 - Site-specific recombinase, at 40,972 to 42,945
GFF6248
GFF6249 - Transporter, monovalent cation:proton antiporter-2 (CPA2) family, at 42,942 to 44,213
GFF6249
GFF6250 - Glutamate--cysteine ligase-like protein YbdK, at 44,210 to 45,430
GFF6250
Position (kb)
42
43
44
45 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 41.959 kb on + strand, within GFF6248 at 41.959 kb on + strand, within GFF6248 at 41.959 kb on + strand, within GFF6248 at 41.959 kb on + strand, within GFF6248 at 41.959 kb on + strand, within GFF6248 at 41.959 kb on + strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.960 kb on - strand, within GFF6248 at 41.983 kb on + strand, within GFF6248 at 41.983 kb on + strand, within GFF6248 at 41.984 kb on - strand, within GFF6248 at 41.984 kb on - strand, within GFF6248 at 41.984 kb on - strand, within GFF6248 at 41.984 kb on - strand, within GFF6248 at 41.984 kb on - strand, within GFF6248 at 42.058 kb on + strand, within GFF6248 at 42.058 kb on + strand, within GFF6248 at 42.058 kb on + strand, within GFF6248 at 42.058 kb on + strand, within GFF6248 at 42.058 kb on + strand, within GFF6248 at 42.059 kb on - strand, within GFF6248 at 42.059 kb on - strand, within GFF6248 at 42.059 kb on - strand, within GFF6248 at 42.109 kb on + strand, within GFF6248 at 42.109 kb on + strand, within GFF6248 at 42.109 kb on + strand, within GFF6248 at 42.109 kb on + strand, within GFF6248 at 42.110 kb on - strand, within GFF6248 at 42.110 kb on - strand, within GFF6248 at 42.110 kb on - strand, within GFF6248 at 42.424 kb on + strand, within GFF6248 at 42.425 kb on - strand, within GFF6248 at 42.425 kb on - strand, within GFF6248 at 42.523 kb on + strand, within GFF6248 at 42.523 kb on + strand, within GFF6248 at 42.523 kb on + strand, within GFF6248 at 42.523 kb on + strand, within GFF6248 at 42.935 kb on + strand at 42.935 kb on + strand at 42.935 kb on + strand at 42.935 kb on + strand at 42.936 kb on - strand at 42.936 kb on - strand at 42.936 kb on - strand at 43.078 kb on - strand, within GFF6249 at 43.078 kb on - strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.299 kb on + strand, within GFF6249 at 43.300 kb on - strand, within GFF6249 at 43.300 kb on - strand, within GFF6249 at 43.311 kb on + strand, within GFF6249 at 43.312 kb on - strand, within GFF6249 at 43.312 kb on - strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.476 kb on + strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.477 kb on - strand, within GFF6249 at 43.495 kb on - strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 43.666 kb on + strand, within GFF6249 at 44.390 kb on + strand, within GFF6250 at 44.391 kb on - strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.639 kb on + strand, within GFF6250 at 44.640 kb on - strand, within GFF6250 at 44.675 kb on + strand, within GFF6250 at 44.675 kb on + strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.676 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.778 kb on - strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.781 kb on + strand, within GFF6250 at 44.782 kb on - strand, within GFF6250 at 44.928 kb on - strand, within GFF6250 at 44.928 kb on - strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.074 kb on + strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.075 kb on - strand, within GFF6250 at 45.137 kb on + strand, within GFF6250 at 45.147 kb on - strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.152 kb on + strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.153 kb on - strand, within GFF6250 at 45.194 kb on + strand, within GFF6250 at 45.194 kb on + strand, within GFF6250 at 45.195 kb on - strand, within GFF6250
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 41,959 + GFF6248 0.50 +0.4 41,959 + GFF6248 0.50 -0.2 41,959 + GFF6248 0.50 -0.8 41,959 + GFF6248 0.50 -0.2 41,959 + GFF6248 0.50 -0.2 41,959 + GFF6248 0.50 +0.1 41,960 - GFF6248 0.50 +0.3 41,960 - GFF6248 0.50 -0.9 41,960 - GFF6248 0.50 -0.4 41,960 - GFF6248 0.50 -1.9 41,960 - GFF6248 0.50 -0.1 41,960 - GFF6248 0.50 +0.6 41,960 - GFF6248 0.50 +0.7 41,960 - GFF6248 0.50 -0.4 41,960 - GFF6248 0.50 +0.2 41,960 - GFF6248 0.50 +0.5 41,960 - GFF6248 0.50 -0.4 41,983 + GFF6248 0.51 -0.9 41,983 + GFF6248 0.51 -0.2 41,984 - GFF6248 0.51 +0.9 41,984 - GFF6248 0.51 -0.5 41,984 - GFF6248 0.51 -1.4 41,984 - GFF6248 0.51 -0.4 41,984 - GFF6248 0.51 -0.8 42,058 + GFF6248 0.55 +0.6 42,058 + GFF6248 0.55 +0.3 42,058 + GFF6248 0.55 +0.0 42,058 + GFF6248 0.55 +0.6 42,058 + GFF6248 0.55 +1.1 42,059 - GFF6248 0.55 +0.5 42,059 - GFF6248 0.55 -1.0 42,059 - GFF6248 0.55 -0.1 42,109 + GFF6248 0.58 -0.6 42,109 + GFF6248 0.58 -0.3 42,109 + GFF6248 0.58 -0.8 42,109 + GFF6248 0.58 -0.6 42,110 - GFF6248 0.58 -0.9 42,110 - GFF6248 0.58 +0.2 42,110 - GFF6248 0.58 -0.9 42,424 + GFF6248 0.74 -1.7 42,425 - GFF6248 0.74 -0.0 42,425 - GFF6248 0.74 +0.1 42,523 + GFF6248 0.79 +1.2 42,523 + GFF6248 0.79 -0.5 42,523 + GFF6248 0.79 +0.3 42,523 + GFF6248 0.79 -0.2 42,935 + +0.3 42,935 + +0.1 42,935 + -0.4 42,935 + +1.9 42,936 - +0.1 42,936 - +0.0 42,936 - -0.6 43,078 - GFF6249 0.11 -2.6 43,078 - GFF6249 0.11 -2.1 43,299 + GFF6249 0.28 -1.4 43,299 + GFF6249 0.28 -1.1 43,299 + GFF6249 0.28 -1.7 43,299 + GFF6249 0.28 +0.2 43,300 - GFF6249 0.28 +0.3 43,300 - GFF6249 0.28 -0.9 43,311 + GFF6249 0.29 -1.2 43,312 - GFF6249 0.29 -1.0 43,312 - GFF6249 0.29 -1.1 43,476 + GFF6249 0.42 -2.0 43,476 + GFF6249 0.42 -3.9 43,476 + GFF6249 0.42 -1.9 43,476 + GFF6249 0.42 -1.4 43,476 + GFF6249 0.42 -1.8 43,476 + GFF6249 0.42 -1.9 43,477 - GFF6249 0.42 -0.5 43,477 - GFF6249 0.42 -1.4 43,477 - GFF6249 0.42 -0.3 43,477 - GFF6249 0.42 +1.0 43,495 - GFF6249 0.43 -2.7 43,666 + GFF6249 0.57 -1.7 43,666 + GFF6249 0.57 -1.5 43,666 + GFF6249 0.57 -2.4 43,666 + GFF6249 0.57 -0.8 44,390 + GFF6250 0.15 -1.5 44,391 - GFF6250 0.15 -1.6 44,639 + GFF6250 0.35 -1.6 44,639 + GFF6250 0.35 -2.3 44,639 + GFF6250 0.35 -1.7 44,639 + GFF6250 0.35 -1.3 44,640 - GFF6250 0.35 -0.7 44,675 + GFF6250 0.38 -0.4 44,675 + GFF6250 0.38 -1.9 44,676 - GFF6250 0.38 -1.3 44,676 - GFF6250 0.38 -1.0 44,676 - GFF6250 0.38 -0.8 44,676 - GFF6250 0.38 -1.7 44,778 - GFF6250 0.47 -0.8 44,778 - GFF6250 0.47 -0.9 44,778 - GFF6250 0.47 -0.9 44,781 + GFF6250 0.47 -1.7 44,781 + GFF6250 0.47 -1.8 44,781 + GFF6250 0.47 -2.3 44,781 + GFF6250 0.47 -1.1 44,782 - GFF6250 0.47 -1.9 44,928 - GFF6250 0.59 +0.0 44,928 - GFF6250 0.59 -1.0 45,074 + GFF6250 0.71 -2.0 45,074 + GFF6250 0.71 -1.7 45,074 + GFF6250 0.71 -1.7 45,074 + GFF6250 0.71 -1.7 45,075 - GFF6250 0.71 -2.9 45,075 - GFF6250 0.71 -0.8 45,075 - GFF6250 0.71 -2.0 45,075 - GFF6250 0.71 -2.9 45,137 + GFF6250 0.76 -1.0 45,147 - GFF6250 0.77 -0.8 45,152 + GFF6250 0.77 -2.1 45,152 + GFF6250 0.77 -0.9 45,152 + GFF6250 0.77 -2.2 45,152 + GFF6250 0.77 -1.3 45,152 + GFF6250 0.77 +0.4 45,153 - GFF6250 0.77 +1.7 45,153 - GFF6250 0.77 -0.8 45,153 - GFF6250 0.77 -0.7 45,153 - GFF6250 0.77 -1.8 45,153 - GFF6250 0.77 -2.3 45,194 + GFF6250 0.81 -1.6 45,194 + GFF6250 0.81 -1.4 45,195 - GFF6250 0.81 -0.3
Or see this region's nucleotide sequence