Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6162 and GFF6163 are separated by 11 nucleotides GFF6163 and GFF6164 are separated by 4 nucleotides GFF6164 and GFF6165 overlap by 4 nucleotides GFF6165 and GFF6166 are separated by 49 nucleotides
GFF6162 - Dihydrodipicolinate synthase family protein RB7999, at 67,753 to 68,820
GFF6162
GFF6163 - hypothetical protein, at 68,832 to 69,686
GFF6163
GFF6164 - FIG01094124: hypothetical protein, at 69,691 to 70,740
GFF6164
GFF6165 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 70,737 to 71,603
GFF6165
GFF6166 - Transcriptional regulator, AcrR family, at 71,653 to 72,312
GFF6166
Position (kb) 69
70
71 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 69.343 kb on + strand, within GFF6163 at 69.343 kb on + strand, within GFF6163 at 69.343 kb on + strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.344 kb on - strand, within GFF6163 at 69.695 kb on + strand at 69.695 kb on + strand at 69.695 kb on + strand at 69.696 kb on - strand at 69.696 kb on - strand at 69.734 kb on + strand at 69.735 kb on - strand at 69.735 kb on - strand at 69.890 kb on + strand, within GFF6164 at 69.890 kb on + strand, within GFF6164 at 69.890 kb on + strand, within GFF6164 at 69.891 kb on - strand, within GFF6164 at 69.891 kb on - strand, within GFF6164 at 70.115 kb on + strand, within GFF6164 at 70.115 kb on + strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.116 kb on - strand, within GFF6164 at 70.163 kb on + strand, within GFF6164 at 70.163 kb on + strand, within GFF6164 at 70.164 kb on - strand, within GFF6164 at 70.203 kb on - strand, within GFF6164 at 70.203 kb on - strand, within GFF6164 at 70.268 kb on + strand, within GFF6164 at 70.668 kb on - strand at 70.668 kb on - strand at 70.796 kb on - strand at 70.796 kb on - strand at 70.972 kb on + strand, within GFF6165 at 70.972 kb on + strand, within GFF6165 at 71.146 kb on + strand, within GFF6165 at 71.147 kb on - strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.344 kb on + strand, within GFF6165 at 71.345 kb on - strand, within GFF6165 at 71.345 kb on - strand, within GFF6165 at 71.567 kb on - strand at 71.582 kb on - strand at 71.603 kb on + strand at 71.603 kb on + strand at 71.603 kb on + strand at 71.604 kb on - strand at 71.604 kb on - strand at 71.604 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 69,343 + GFF6163 0.60 +0.1 69,343 + GFF6163 0.60 +0.6 69,343 + GFF6163 0.60 -0.3 69,344 - GFF6163 0.60 -0.6 69,344 - GFF6163 0.60 +0.6 69,344 - GFF6163 0.60 +0.1 69,695 + +1.0 69,695 + -0.6 69,695 + -0.3 69,696 - +0.4 69,696 - -1.5 69,734 + -0.0 69,735 - -2.0 69,735 - -0.3 69,890 + GFF6164 0.19 +0.5 69,890 + GFF6164 0.19 -1.9 69,890 + GFF6164 0.19 +0.4 69,891 - GFF6164 0.19 -0.3 69,891 - GFF6164 0.19 -0.2 70,115 + GFF6164 0.40 -0.8 70,115 + GFF6164 0.40 -1.5 70,116 - GFF6164 0.40 -0.3 70,116 - GFF6164 0.40 +1.7 70,116 - GFF6164 0.40 -1.7 70,116 - GFF6164 0.40 +0.5 70,116 - GFF6164 0.40 -0.6 70,163 + GFF6164 0.45 +0.1 70,163 + GFF6164 0.45 -2.7 70,164 - GFF6164 0.45 -0.2 70,203 - GFF6164 0.49 -2.3 70,203 - GFF6164 0.49 -0.4 70,268 + GFF6164 0.55 +0.2 70,668 - +0.3 70,668 - -0.2 70,796 - -0.2 70,796 - -1.0 70,972 + GFF6165 0.27 -0.5 70,972 + GFF6165 0.27 -0.2 71,146 + GFF6165 0.47 -0.8 71,147 - GFF6165 0.47 -0.8 71,344 + GFF6165 0.70 -0.5 71,344 + GFF6165 0.70 +0.7 71,344 + GFF6165 0.70 +0.4 71,344 + GFF6165 0.70 +0.2 71,344 + GFF6165 0.70 +0.4 71,344 + GFF6165 0.70 +0.3 71,344 + GFF6165 0.70 +0.3 71,345 - GFF6165 0.70 +0.4 71,345 - GFF6165 0.70 +0.7 71,567 - -1.0 71,582 - +0.4 71,603 + -3.3 71,603 + -0.1 71,603 + -1.6 71,604 - -1.3 71,604 - -0.9 71,604 - -0.5
Or see this region's nucleotide sequence