Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6092 and GFF6093 are separated by 44 nucleotides GFF6093 and GFF6094 overlap by 4 nucleotides GFF6094 and GFF6095 are separated by 14 nucleotides
GFF6092 - Penicillin G acylase precursor (EC 3.5.1.11), at 152,738 to 155,182
GFF6092
GFF6093 - 1,2-phenylacetyl-CoA epoxidase, subunit A (EC 1.14.13.149), at 155,227 to 156,237
GFF6093
GFF6094 - 1,2-phenylacetyl-CoA epoxidase, subunit B (EC 1.14.13.149), at 156,234 to 156,530
GFF6094
GFF6095 - 1,2-phenylacetyl-CoA epoxidase, subunit C (EC 1.14.13.149), at 156,545 to 157,324
GFF6095
Position (kb) 155
156
157 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 154.248 kb on - strand, within GFF6092 at 154.248 kb on - strand, within GFF6092 at 154.304 kb on + strand, within GFF6092 at 154.304 kb on + strand, within GFF6092 at 154.305 kb on - strand, within GFF6092 at 154.305 kb on - strand, within GFF6092 at 154.404 kb on - strand, within GFF6092 at 154.404 kb on - strand, within GFF6092 at 154.404 kb on - strand, within GFF6092 at 154.424 kb on + strand, within GFF6092 at 154.424 kb on + strand, within GFF6092 at 154.425 kb on - strand, within GFF6092 at 154.425 kb on - strand, within GFF6092 at 154.425 kb on - strand, within GFF6092 at 154.547 kb on + strand, within GFF6092 at 154.547 kb on + strand, within GFF6092 at 154.548 kb on - strand, within GFF6092 at 154.548 kb on - strand, within GFF6092 at 154.595 kb on + strand, within GFF6092 at 154.644 kb on - strand, within GFF6092 at 154.659 kb on - strand, within GFF6092 at 154.659 kb on - strand, within GFF6092 at 154.715 kb on + strand, within GFF6092 at 154.716 kb on - strand, within GFF6092 at 154.716 kb on - strand, within GFF6092 at 154.716 kb on - strand, within GFF6092 at 155.075 kb on + strand at 155.075 kb on + strand at 155.076 kb on - strand at 155.076 kb on - strand at 155.076 kb on - strand at 155.166 kb on + strand at 155.166 kb on + strand at 155.166 kb on + strand at 155.166 kb on + strand at 155.167 kb on - strand at 155.167 kb on - strand at 155.167 kb on - strand at 155.177 kb on + strand at 155.178 kb on - strand at 155.231 kb on - strand at 155.536 kb on + strand, within GFF6093 at 155.536 kb on + strand, within GFF6093 at 155.536 kb on + strand, within GFF6093 at 155.536 kb on + strand, within GFF6093 at 155.537 kb on - strand, within GFF6093 at 155.542 kb on + strand, within GFF6093 at 155.542 kb on + strand, within GFF6093 at 155.614 kb on + strand, within GFF6093 at 155.615 kb on - strand, within GFF6093 at 155.615 kb on - strand, within GFF6093 at 155.725 kb on + strand, within GFF6093 at 156.369 kb on + strand, within GFF6094 at 156.369 kb on + strand, within GFF6094 at 156.370 kb on - strand, within GFF6094 at 156.481 kb on - strand, within GFF6094 at 156.499 kb on - strand, within GFF6094 at 156.499 kb on - strand, within GFF6094 at 156.587 kb on + strand at 156.588 kb on - strand at 156.806 kb on + strand, within GFF6095 at 156.807 kb on - strand, within GFF6095 at 157.070 kb on + strand, within GFF6095 at 157.070 kb on + strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.071 kb on - strand, within GFF6095 at 157.071 kb on - strand, within GFF6095
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 154,248 - GFF6092 0.62 +2.2 154,248 - GFF6092 0.62 +0.2 154,304 + GFF6092 0.64 +0.3 154,304 + GFF6092 0.64 +0.5 154,305 - GFF6092 0.64 -0.6 154,305 - GFF6092 0.64 +0.1 154,404 - GFF6092 0.68 +0.2 154,404 - GFF6092 0.68 -1.0 154,404 - GFF6092 0.68 -0.0 154,424 + GFF6092 0.69 -0.0 154,424 + GFF6092 0.69 +0.1 154,425 - GFF6092 0.69 -0.9 154,425 - GFF6092 0.69 +0.8 154,425 - GFF6092 0.69 -0.4 154,547 + GFF6092 0.74 +0.2 154,547 + GFF6092 0.74 -0.0 154,548 - GFF6092 0.74 -0.4 154,548 - GFF6092 0.74 +0.4 154,595 + GFF6092 0.76 +0.5 154,644 - GFF6092 0.78 +0.3 154,659 - GFF6092 0.79 -1.4 154,659 - GFF6092 0.79 +0.1 154,715 + GFF6092 0.81 +1.1 154,716 - GFF6092 0.81 -1.4 154,716 - GFF6092 0.81 -0.5 154,716 - GFF6092 0.81 -0.0 155,075 + -0.4 155,075 + +0.7 155,076 - +0.4 155,076 - -2.3 155,076 - -0.6 155,166 + -0.8 155,166 + -0.3 155,166 + -0.4 155,166 + -0.0 155,167 - -0.4 155,167 - -0.5 155,167 - -0.4 155,177 + -0.8 155,178 - -0.4 155,231 - -0.0 155,536 + GFF6093 0.31 +0.9 155,536 + GFF6093 0.31 +1.6 155,536 + GFF6093 0.31 -2.8 155,536 + GFF6093 0.31 -0.2 155,537 - GFF6093 0.31 +1.2 155,542 + GFF6093 0.31 +0.3 155,542 + GFF6093 0.31 +0.3 155,614 + GFF6093 0.38 -1.0 155,615 - GFF6093 0.38 -0.8 155,615 - GFF6093 0.38 +0.9 155,725 + GFF6093 0.49 +0.4 156,369 + GFF6094 0.45 -1.1 156,369 + GFF6094 0.45 +0.2 156,370 - GFF6094 0.46 +0.3 156,481 - GFF6094 0.83 -1.4 156,499 - GFF6094 0.89 -1.3 156,499 - GFF6094 0.89 -0.1 156,587 + +1.0 156,588 - +0.1 156,806 + GFF6095 0.33 +0.2 156,807 - GFF6095 0.34 -0.2 157,070 + GFF6095 0.67 -0.8 157,070 + GFF6095 0.67 +0.6 157,071 - GFF6095 0.67 -1.1 157,071 - GFF6095 0.67 +1.2 157,071 - GFF6095 0.67 -0.5 157,071 - GFF6095 0.67 +0.3
Or see this region's nucleotide sequence