Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5984 and GFF5985 are separated by 223 nucleotides GFF5985 and GFF5986 are separated by 77 nucleotides GFF5986 and GFF5987 are separated by 67 nucleotides
GFF5984 - L-lactate dehydrogenase, at 37,474 to 38,631
GFF5984
GFF5985 - dicarboxylic acid transporter PcaT, at 38,855 to 40,090
GFF5985
GFF5986 - hypothetical protein, at 40,168 to 40,761
GFF5986
GFF5987 - Response regulator BaeR, at 40,829 to 41,536
GFF5987
Position (kb)
38
39
40
41 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 37.873 kb on + strand, within GFF5984 at 37.873 kb on + strand, within GFF5984 at 37.873 kb on + strand, within GFF5984 at 37.873 kb on + strand, within GFF5984 at 37.873 kb on + strand, within GFF5984 at 37.873 kb on + strand, within GFF5984 at 37.874 kb on - strand, within GFF5984 at 37.874 kb on - strand, within GFF5984 at 37.874 kb on - strand, within GFF5984 at 38.489 kb on - strand, within GFF5984 at 38.504 kb on - strand, within GFF5984 at 38.816 kb on + strand at 38.816 kb on + strand at 38.816 kb on + strand at 38.897 kb on + strand at 38.898 kb on - strand at 38.898 kb on - strand at 38.903 kb on + strand at 38.903 kb on + strand at 38.903 kb on + strand at 38.903 kb on + strand at 38.903 kb on + strand at 38.904 kb on - strand at 38.904 kb on - strand at 38.904 kb on - strand at 38.904 kb on - strand at 38.922 kb on - strand at 39.128 kb on + strand, within GFF5985 at 39.128 kb on + strand, within GFF5985 at 39.128 kb on + strand, within GFF5985 at 39.129 kb on - strand, within GFF5985 at 39.129 kb on - strand, within GFF5985 at 39.206 kb on + strand, within GFF5985 at 39.224 kb on + strand, within GFF5985 at 39.224 kb on + strand, within GFF5985 at 39.224 kb on + strand, within GFF5985 at 39.224 kb on + strand, within GFF5985 at 39.225 kb on - strand, within GFF5985 at 39.225 kb on - strand, within GFF5985 at 39.578 kb on + strand, within GFF5985 at 39.578 kb on + strand, within GFF5985 at 39.590 kb on + strand, within GFF5985 at 39.632 kb on + strand, within GFF5985 at 39.633 kb on - strand, within GFF5985 at 39.633 kb on - strand, within GFF5985 at 39.633 kb on - strand, within GFF5985 at 39.633 kb on - strand, within GFF5985 at 39.659 kb on + strand, within GFF5985 at 39.660 kb on - strand, within GFF5985 at 39.660 kb on - strand, within GFF5985 at 39.660 kb on - strand, within GFF5985 at 39.660 kb on - strand, within GFF5985 at 39.660 kb on - strand, within GFF5985 at 39.843 kb on - strand, within GFF5985 at 39.843 kb on - strand, within GFF5985 at 39.956 kb on + strand, within GFF5985 at 40.007 kb on + strand at 40.071 kb on - strand at 40.094 kb on + strand at 40.094 kb on + strand at 40.094 kb on + strand at 40.141 kb on + strand at 40.141 kb on + strand at 40.141 kb on + strand at 40.142 kb on - strand at 40.142 kb on - strand at 40.142 kb on - strand at 40.142 kb on - strand at 40.142 kb on - strand at 40.193 kb on + strand at 40.193 kb on + strand at 40.193 kb on + strand at 40.220 kb on - strand at 40.435 kb on + strand, within GFF5986 at 40.633 kb on + strand, within GFF5986 at 40.633 kb on + strand, within GFF5986 at 40.633 kb on + strand, within GFF5986 at 40.633 kb on + strand, within GFF5986 at 40.699 kb on - strand, within GFF5986 at 40.699 kb on - strand, within GFF5986 at 40.699 kb on - strand, within GFF5986 at 40.699 kb on - strand, within GFF5986 at 40.774 kb on + strand at 40.775 kb on - strand at 40.846 kb on + strand at 40.846 kb on + strand at 40.847 kb on - strand at 40.847 kb on - strand at 41.086 kb on + strand, within GFF5987 at 41.086 kb on + strand, within GFF5987 at 41.087 kb on - strand, within GFF5987 at 41.088 kb on + strand, within GFF5987 at 41.088 kb on + strand, within GFF5987 at 41.088 kb on + strand, within GFF5987
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 37,873 + GFF5984 0.34 +1.2 37,873 + GFF5984 0.34 +0.6 37,873 + GFF5984 0.34 +1.4 37,873 + GFF5984 0.34 -1.3 37,873 + GFF5984 0.34 -0.6 37,873 + GFF5984 0.34 +0.0 37,874 - GFF5984 0.35 -1.3 37,874 - GFF5984 0.35 -0.8 37,874 - GFF5984 0.35 -0.4 38,489 - GFF5984 0.88 +0.1 38,504 - GFF5984 0.89 +0.1 38,816 + -0.7 38,816 + -0.4 38,816 + +0.1 38,897 + +0.3 38,898 - -1.1 38,898 - -0.1 38,903 + -0.2 38,903 + +0.6 38,903 + -0.4 38,903 + -0.6 38,903 + +0.8 38,904 - +0.7 38,904 - -0.5 38,904 - -0.3 38,904 - -0.6 38,922 - -0.6 39,128 + GFF5985 0.22 -1.6 39,128 + GFF5985 0.22 +0.5 39,128 + GFF5985 0.22 -0.2 39,129 - GFF5985 0.22 -0.3 39,129 - GFF5985 0.22 +0.1 39,206 + GFF5985 0.28 -1.1 39,224 + GFF5985 0.30 -0.1 39,224 + GFF5985 0.30 +0.1 39,224 + GFF5985 0.30 -0.0 39,224 + GFF5985 0.30 +1.0 39,225 - GFF5985 0.30 -0.2 39,225 - GFF5985 0.30 -0.4 39,578 + GFF5985 0.58 -0.1 39,578 + GFF5985 0.58 -0.7 39,590 + GFF5985 0.59 +0.7 39,632 + GFF5985 0.63 +1.9 39,633 - GFF5985 0.63 +0.2 39,633 - GFF5985 0.63 +1.8 39,633 - GFF5985 0.63 -0.9 39,633 - GFF5985 0.63 -0.3 39,659 + GFF5985 0.65 +0.1 39,660 - GFF5985 0.65 -0.3 39,660 - GFF5985 0.65 -0.4 39,660 - GFF5985 0.65 +0.4 39,660 - GFF5985 0.65 -0.4 39,660 - GFF5985 0.65 +0.1 39,843 - GFF5985 0.80 -0.2 39,843 - GFF5985 0.80 +0.4 39,956 + GFF5985 0.89 -0.5 40,007 + +0.3 40,071 - -0.5 40,094 + +0.1 40,094 + +0.4 40,094 + -0.7 40,141 + +1.8 40,141 + +0.2 40,141 + -0.4 40,142 - +1.0 40,142 - +0.1 40,142 - -0.4 40,142 - -0.2 40,142 - +0.3 40,193 + -0.4 40,193 + -0.8 40,193 + -0.2 40,220 - +0.3 40,435 + GFF5986 0.45 -0.5 40,633 + GFF5986 0.78 +0.2 40,633 + GFF5986 0.78 -0.5 40,633 + GFF5986 0.78 -0.5 40,633 + GFF5986 0.78 -0.3 40,699 - GFF5986 0.89 -0.2 40,699 - GFF5986 0.89 -0.6 40,699 - GFF5986 0.89 +0.4 40,699 - GFF5986 0.89 -0.4 40,774 + -0.5 40,775 - +0.5 40,846 + -0.7 40,846 + -1.0 40,847 - -0.4 40,847 - -2.4 41,086 + GFF5987 0.36 -0.6 41,086 + GFF5987 0.36 +0.4 41,087 - GFF5987 0.36 +0.2 41,088 + GFF5987 0.37 -0.5 41,088 + GFF5987 0.37 -0.8 41,088 + GFF5987 0.37 -0.1
Or see this region's nucleotide sequence