Strain Fitness in Variovorax sp. SCN45 around GFF5947

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5947 and GFF5948 are separated by 22 nucleotidesGFF5948 and GFF5949 are separated by 3 nucleotides GFF5947 - Large extracellular alpha-helical protein, at 94 to 6,120 GFF5947 GFF5948 - hypothetical protein, at 6,143 to 6,790 GFF5948 GFF5949 - Glyoxalase family protein, at 6,794 to 7,207 GFF5949 Position (kb) 0 1 2 3 4 5 6 7Strain fitness (log2 ratio) -2 -1 0 1 2at 0.060 kb on + strandat 0.075 kb on + strandat 0.075 kb on + strandat 0.086 kb on - strandat 0.478 kb on + strandat 0.478 kb on + strandat 0.479 kb on - strandat 0.479 kb on - strandat 0.479 kb on - strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.493 kb on + strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.494 kb on - strandat 0.751 kb on + strand, within GFF5947at 1.030 kb on + strand, within GFF5947at 1.030 kb on + strand, within GFF5947at 1.030 kb on + strand, within GFF5947at 1.030 kb on + strand, within GFF5947at 1.031 kb on - strand, within GFF5947at 1.031 kb on - strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.369 kb on + strand, within GFF5947at 1.370 kb on - strand, within GFF5947at 1.370 kb on - strand, within GFF5947at 1.370 kb on - strand, within GFF5947at 1.370 kb on - strand, within GFF5947at 1.630 kb on + strand, within GFF5947at 1.631 kb on - strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.651 kb on + strand, within GFF5947at 1.652 kb on - strand, within GFF5947at 1.652 kb on - strand, within GFF5947at 1.652 kb on - strand, within GFF5947at 1.652 kb on - strand, within GFF5947at 1.885 kb on + strand, within GFF5947at 1.885 kb on + strand, within GFF5947at 2.278 kb on + strand, within GFF5947at 2.279 kb on - strand, within GFF5947at 2.356 kb on + strand, within GFF5947at 2.357 kb on - strand, within GFF5947at 2.357 kb on - strand, within GFF5947at 2.491 kb on + strand, within GFF5947at 2.491 kb on + strand, within GFF5947at 2.491 kb on + strand, within GFF5947at 2.492 kb on - strand, within GFF5947at 2.492 kb on - strand, within GFF5947at 2.492 kb on - strand, within GFF5947at 2.492 kb on - strand, within GFF5947at 2.573 kb on - strand, within GFF5947at 2.804 kb on - strand, within GFF5947at 3.035 kb on - strand, within GFF5947at 3.035 kb on - strand, within GFF5947at 3.035 kb on - strand, within GFF5947at 3.208 kb on + strand, within GFF5947at 3.286 kb on + strand, within GFF5947at 3.286 kb on + strand, within GFF5947at 3.286 kb on + strand, within GFF5947at 3.286 kb on + strand, within GFF5947at 3.286 kb on + strand, within GFF5947at 3.287 kb on - strand, within GFF5947at 3.287 kb on - strand, within GFF5947at 3.454 kb on + strand, within GFF5947at 3.454 kb on + strand, within GFF5947at 3.502 kb on + strand, within GFF5947at 3.503 kb on - strand, within GFF5947at 3.560 kb on - strand, within GFF5947at 3.575 kb on - strand, within GFF5947at 3.575 kb on - strand, within GFF5947at 3.575 kb on - strand, within GFF5947at 3.575 kb on - strand, within GFF5947at 4.711 kb on + strand, within GFF5947at 4.711 kb on + strand, within GFF5947at 4.738 kb on + strand, within GFF5947at 4.738 kb on + strand, within GFF5947at 4.738 kb on + strand, within GFF5947at 4.739 kb on - strand, within GFF5947at 4.739 kb on - strand, within GFF5947at 4.792 kb on + strand, within GFF5947at 5.189 kb on - strand, within GFF5947at 5.189 kb on - strand, within GFF5947at 5.695 kb on + strandat 5.747 kb on - strandat 5.747 kb on - strandat 5.747 kb on - strandat 5.956 kb on + strandat 5.957 kb on - strandat 5.957 kb on - strandat 5.959 kb on + strandat 5.959 kb on + strandat 5.960 kb on - strandat 5.960 kb on - strandat 5.965 kb on + strandat 5.966 kb on - strandat 5.966 kb on - strandat 5.995 kb on + strandat 5.995 kb on + strandat 5.995 kb on + strandat 5.995 kb on + strandat 5.995 kb on + strandat 5.996 kb on - strandat 5.996 kb on - strandat 6.058 kb on + strandat 6.059 kb on - strandat 6.059 kb on - strandat 6.118 kb on + strandat 6.119 kb on - strandat 6.329 kb on + strand, within GFF5948at 6.344 kb on + strand, within GFF5948at 6.345 kb on - strand, within GFF5948at 6.359 kb on + strand, within GFF5948at 6.452 kb on + strand, within GFF5948at 6.452 kb on + strand, within GFF5948at 6.452 kb on + strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.453 kb on - strand, within GFF5948at 6.455 kb on - strand, within GFF5948at 6.455 kb on - strand, within GFF5948at 6.500 kb on + strand, within GFF5948at 6.501 kb on - strand, within GFF5948at 6.501 kb on - strand, within GFF5948at 6.531 kb on - strand, within GFF5948at 6.716 kb on + strand, within GFF5948at 6.717 kb on - strand, within GFF5948at 6.717 kb on - strand, within GFF5948

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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60 + -0.5
75 + -2.0
75 + -0.4
86 - +1.6
478 + -1.2
478 + +0.6
479 - +0.3
479 - -0.9
479 - +0.1
493 + -0.9
493 + -0.0
493 + +0.2
494 - +0.4
494 - -0.4
494 - -0.4
751 + GFF5947 0.11 -1.0
1,030 + GFF5947 0.16 +1.5
1,030 + GFF5947 0.16 +0.3
1,030 + GFF5947 0.16 +0.1
1,030 + GFF5947 0.16 +0.5
1,031 - GFF5947 0.16 -0.5
1,031 - GFF5947 0.16 -1.4
1,369 + GFF5947 0.21 -0.0
1,369 + GFF5947 0.21 -0.2
1,369 + GFF5947 0.21 -0.0
1,369 + GFF5947 0.21 +0.6
1,369 + GFF5947 0.21 -1.0
1,369 + GFF5947 0.21 -0.4
1,369 + GFF5947 0.21 +2.6
1,369 + GFF5947 0.21 -0.8
1,369 + GFF5947 0.21 -0.0
1,370 - GFF5947 0.21 -0.3
1,370 - GFF5947 0.21 -0.5
1,370 - GFF5947 0.21 +1.4
1,370 - GFF5947 0.21 -0.2
1,630 + GFF5947 0.25 +0.8
1,631 - GFF5947 0.26 -1.8
1,651 + GFF5947 0.26 -0.8
1,651 + GFF5947 0.26 +0.1
1,651 + GFF5947 0.26 +0.6
1,651 + GFF5947 0.26 -0.4
1,651 + GFF5947 0.26 +2.1
1,651 + GFF5947 0.26 +0.6
1,651 + GFF5947 0.26 -0.2
1,651 + GFF5947 0.26 +0.6
1,651 + GFF5947 0.26 +1.3
1,652 - GFF5947 0.26 -0.2
1,652 - GFF5947 0.26 -0.2
1,652 - GFF5947 0.26 -0.8
1,652 - GFF5947 0.26 -1.0
1,885 + GFF5947 0.30 -0.6
1,885 + GFF5947 0.30 -0.0
2,278 + GFF5947 0.36 -0.5
2,279 - GFF5947 0.36 +0.1
2,356 + GFF5947 0.38 -0.5
2,357 - GFF5947 0.38 +0.1
2,357 - GFF5947 0.38 -0.4
2,491 + GFF5947 0.40 +1.0
2,491 + GFF5947 0.40 -0.4
2,491 + GFF5947 0.40 +0.8
2,492 - GFF5947 0.40 -0.4
2,492 - GFF5947 0.40 -1.5
2,492 - GFF5947 0.40 -0.2
2,492 - GFF5947 0.40 +0.4
2,573 - GFF5947 0.41 +0.4
2,804 - GFF5947 0.45 +1.0
3,035 - GFF5947 0.49 -0.4
3,035 - GFF5947 0.49 +0.2
3,035 - GFF5947 0.49 +0.3
3,208 + GFF5947 0.52 +0.9
3,286 + GFF5947 0.53 +1.2
3,286 + GFF5947 0.53 -0.0
3,286 + GFF5947 0.53 -0.1
3,286 + GFF5947 0.53 -0.5
3,286 + GFF5947 0.53 +0.9
3,287 - GFF5947 0.53 +0.6
3,287 - GFF5947 0.53 -0.1
3,454 + GFF5947 0.56 +1.1
3,454 + GFF5947 0.56 -0.0
3,502 + GFF5947 0.57 -0.2
3,503 - GFF5947 0.57 -0.7
3,560 - GFF5947 0.58 -0.1
3,575 - GFF5947 0.58 -0.0
3,575 - GFF5947 0.58 -0.4
3,575 - GFF5947 0.58 +0.1
3,575 - GFF5947 0.58 -0.7
4,711 + GFF5947 0.77 +0.0
4,711 + GFF5947 0.77 +0.3
4,738 + GFF5947 0.77 -0.2
4,738 + GFF5947 0.77 -1.6
4,738 + GFF5947 0.77 -0.1
4,739 - GFF5947 0.77 -0.4
4,739 - GFF5947 0.77 -0.3
4,792 + GFF5947 0.78 -0.2
5,189 - GFF5947 0.85 -0.2
5,189 - GFF5947 0.85 -0.6
5,695 + -1.1
5,747 - -2.0
5,747 - +0.3
5,747 - -0.4
5,956 + -0.0
5,957 - +0.2
5,957 - -1.1
5,959 + +0.4
5,959 + +0.2
5,960 - -0.1
5,960 - -0.1
5,965 + -0.6
5,966 - -0.5
5,966 - -0.3
5,995 + -0.2
5,995 + +0.1
5,995 + -0.6
5,995 + +1.4
5,995 + -0.0
5,996 - -0.9
5,996 - -0.1
6,058 + +0.1
6,059 - -1.6
6,059 - +0.8
6,118 + +0.6
6,119 - +0.4
6,329 + GFF5948 0.29 +0.6
6,344 + GFF5948 0.31 +0.2
6,345 - GFF5948 0.31 -1.3
6,359 + GFF5948 0.33 -1.1
6,452 + GFF5948 0.48 -0.2
6,452 + GFF5948 0.48 +0.6
6,452 + GFF5948 0.48 +0.6
6,453 - GFF5948 0.48 -0.0
6,453 - GFF5948 0.48 -0.3
6,453 - GFF5948 0.48 +0.1
6,453 - GFF5948 0.48 +0.6
6,453 - GFF5948 0.48 -0.2
6,455 - GFF5948 0.48 +1.2
6,455 - GFF5948 0.48 +1.6
6,500 + GFF5948 0.55 +1.0
6,501 - GFF5948 0.55 -0.0
6,501 - GFF5948 0.55 -0.1
6,531 - GFF5948 0.60 -0.8
6,716 + GFF5948 0.88 +0.3
6,717 - GFF5948 0.89 +0.0
6,717 - GFF5948 0.89 -0.5

Or see this region's nucleotide sequence