Strain Fitness in Variovorax sp. SCN45 around GFF593

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF591 and GFF592 are separated by 7 nucleotidesGFF592 and GFF593 overlap by 1 nucleotidesGFF593 and GFF594 are separated by 143 nucleotides GFF591 - Renalase (EC 1.6.3.5), oxidases 1,2-dihydro- and 1,6-dihydro- beta-NAD(P)H isomers back to NAD(P), at 637,621 to 638,721 GFF591 GFF592 - Glutamyl-Q tRNA(Asp) synthetase, at 638,729 to 639,631 GFF592 GFF593 - tRNA (guanine(46)-N(7))-methyltransferase (EC 2.1.1.33), at 639,631 to 640,389 GFF593 GFF594 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 640,533 to 641,654 GFF594 Position (kb) 639 640 641Strain fitness (log2 ratio) -2 -1 0 1 2at 638.978 kb on + strand, within GFF592at 638.978 kb on + strand, within GFF592at 638.978 kb on + strand, within GFF592at 638.978 kb on + strand, within GFF592at 638.979 kb on - strand, within GFF592at 638.979 kb on - strand, within GFF592at 639.104 kb on + strand, within GFF592at 639.284 kb on + strand, within GFF592at 639.284 kb on + strand, within GFF592at 639.285 kb on - strand, within GFF592at 639.285 kb on - strand, within GFF592at 639.285 kb on - strand, within GFF592at 639.413 kb on + strand, within GFF592at 639.413 kb on + strand, within GFF592at 639.413 kb on + strand, within GFF592at 639.414 kb on - strand, within GFF592at 639.414 kb on - strand, within GFF592at 639.414 kb on - strand, within GFF592at 639.614 kb on + strandat 639.615 kb on - strandat 639.630 kb on - strandat 639.630 kb on - strandat 639.805 kb on + strand, within GFF593at 639.805 kb on + strand, within GFF593at 639.805 kb on + strand, within GFF593at 639.805 kb on + strand, within GFF593at 639.806 kb on - strand, within GFF593at 639.806 kb on - strand, within GFF593at 639.806 kb on - strand, within GFF593at 639.806 kb on - strand, within GFF593at 639.806 kb on - strand, within GFF593at 639.817 kb on + strand, within GFF593at 639.817 kb on + strand, within GFF593at 639.818 kb on - strand, within GFF593at 639.818 kb on - strand, within GFF593at 639.818 kb on - strand, within GFF593at 639.818 kb on - strand, within GFF593at 640.195 kb on + strand, within GFF593at 640.195 kb on + strand, within GFF593at 640.195 kb on + strand, within GFF593at 640.195 kb on + strand, within GFF593at 640.195 kb on + strand, within GFF593at 640.195 kb on + strand, within GFF593at 640.196 kb on - strand, within GFF593at 640.225 kb on + strand, within GFF593at 640.225 kb on + strand, within GFF593at 640.226 kb on - strand, within GFF593at 640.288 kb on + strand, within GFF593at 640.288 kb on + strand, within GFF593at 640.288 kb on + strand, within GFF593at 640.289 kb on - strand, within GFF593at 640.289 kb on - strand, within GFF593at 640.289 kb on - strand, within GFF593at 640.289 kb on - strand, within GFF593at 640.289 kb on - strand, within GFF593at 640.307 kb on - strand, within GFF593at 640.388 kb on - strandat 640.451 kb on - strandat 640.506 kb on + strandat 640.506 kb on + strandat 640.506 kb on + strandat 640.506 kb on + strandat 640.507 kb on - strandat 640.507 kb on - strandat 640.507 kb on - strandat 640.507 kb on - strandat 640.507 kb on - strandat 640.657 kb on + strand, within GFF594at 640.657 kb on + strand, within GFF594at 640.657 kb on + strand, within GFF594at 640.658 kb on - strand, within GFF594at 640.658 kb on - strand, within GFF594at 640.658 kb on - strand, within GFF594at 640.658 kb on - strand, within GFF594at 640.762 kb on - strand, within GFF594at 641.259 kb on + strand, within GFF594at 641.259 kb on + strand, within GFF594at 641.259 kb on + strand, within GFF594at 641.260 kb on - strand, within GFF594

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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638,978 + GFF592 0.28 -0.0
638,978 + GFF592 0.28 -0.3
638,978 + GFF592 0.28 -0.2
638,978 + GFF592 0.28 -0.4
638,979 - GFF592 0.28 +0.6
638,979 - GFF592 0.28 -0.4
639,104 + GFF592 0.42 +0.0
639,284 + GFF592 0.61 -0.4
639,284 + GFF592 0.61 +0.2
639,285 - GFF592 0.62 -0.1
639,285 - GFF592 0.62 -0.2
639,285 - GFF592 0.62 +0.4
639,413 + GFF592 0.76 -0.5
639,413 + GFF592 0.76 -2.1
639,413 + GFF592 0.76 -0.2
639,414 - GFF592 0.76 +0.0
639,414 - GFF592 0.76 +0.0
639,414 - GFF592 0.76 -0.6
639,614 + -0.6
639,615 - +0.5
639,630 - +1.3
639,630 - -1.6
639,805 + GFF593 0.23 +0.5
639,805 + GFF593 0.23 +0.4
639,805 + GFF593 0.23 +0.2
639,805 + GFF593 0.23 +0.5
639,806 - GFF593 0.23 +0.1
639,806 - GFF593 0.23 +0.1
639,806 - GFF593 0.23 +0.3
639,806 - GFF593 0.23 +0.8
639,806 - GFF593 0.23 +0.5
639,817 + GFF593 0.25 +0.2
639,817 + GFF593 0.25 +0.4
639,818 - GFF593 0.25 +1.6
639,818 - GFF593 0.25 +0.3
639,818 - GFF593 0.25 +0.2
639,818 - GFF593 0.25 -1.1
640,195 + GFF593 0.74 -1.3
640,195 + GFF593 0.74 -0.4
640,195 + GFF593 0.74 +1.4
640,195 + GFF593 0.74 +0.1
640,195 + GFF593 0.74 +0.8
640,195 + GFF593 0.74 -0.2
640,196 - GFF593 0.74 +0.1
640,225 + GFF593 0.78 -0.8
640,225 + GFF593 0.78 -1.6
640,226 - GFF593 0.78 -0.0
640,288 + GFF593 0.87 -0.2
640,288 + GFF593 0.87 +1.2
640,288 + GFF593 0.87 -0.2
640,289 - GFF593 0.87 -0.2
640,289 - GFF593 0.87 +0.7
640,289 - GFF593 0.87 +0.7
640,289 - GFF593 0.87 +0.3
640,289 - GFF593 0.87 +1.0
640,307 - GFF593 0.89 -0.2
640,388 - -1.4
640,451 - +1.2
640,506 + +0.1
640,506 + -0.3
640,506 + +2.0
640,506 + -0.2
640,507 - +1.2
640,507 - -0.3
640,507 - -0.5
640,507 - -0.1
640,507 - -0.6
640,657 + GFF594 0.11 -0.5
640,657 + GFF594 0.11 -0.2
640,657 + GFF594 0.11 -2.1
640,658 - GFF594 0.11 +0.7
640,658 - GFF594 0.11 +0.3
640,658 - GFF594 0.11 +0.6
640,658 - GFF594 0.11 -0.4
640,762 - GFF594 0.20 -0.3
641,259 + GFF594 0.65 -0.5
641,259 + GFF594 0.65 +0.2
641,259 + GFF594 0.65 +0.3
641,260 - GFF594 0.65 -0.9

Or see this region's nucleotide sequence