Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF590 and GFF591 are separated by 119 nucleotides GFF591 and GFF592 are separated by 7 nucleotides GFF592 and GFF593 overlap by 1 nucleotides
GFF590 - Transcriptional regulator, LysR family, at 636,557 to 637,501
GFF590
GFF591 - Renalase (EC 1.6.3.5), oxidases 1,2-dihydro- and 1,6-dihydro- beta-NAD(P)H isomers back to NAD(P), at 637,621 to 638,721
GFF591
GFF592 - Glutamyl-Q tRNA(Asp) synthetase, at 638,729 to 639,631
GFF592
GFF593 - tRNA (guanine(46)-N(7))-methyltransferase (EC 2.1.1.33), at 639,631 to 640,389
GFF593
Position (kb) 637
638
639 Strain fitness (log2 ratio) -2
-1
0
1
2 at 636.678 kb on - strand, within GFF590 at 637.094 kb on + strand, within GFF590 at 637.095 kb on - strand, within GFF590 at 637.095 kb on - strand, within GFF590 at 637.328 kb on + strand, within GFF590 at 637.328 kb on + strand, within GFF590 at 637.328 kb on + strand, within GFF590 at 637.329 kb on - strand, within GFF590 at 637.329 kb on - strand, within GFF590 at 637.329 kb on - strand, within GFF590 at 637.530 kb on + strand at 637.567 kb on - strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.570 kb on + strand at 637.571 kb on - strand at 637.571 kb on - strand at 637.571 kb on - strand at 637.571 kb on - strand at 637.571 kb on - strand at 637.571 kb on - strand at 637.571 kb on - strand at 637.702 kb on + strand at 637.702 kb on + strand at 637.702 kb on + strand at 637.702 kb on + strand at 637.702 kb on + strand at 637.702 kb on + strand at 637.703 kb on - strand at 637.703 kb on - strand at 637.703 kb on - strand at 637.703 kb on - strand at 637.858 kb on + strand, within GFF591 at 637.858 kb on + strand, within GFF591 at 637.859 kb on - strand, within GFF591 at 638.164 kb on + strand, within GFF591 at 638.164 kb on + strand, within GFF591 at 638.165 kb on - strand, within GFF591 at 638.165 kb on - strand, within GFF591 at 638.165 kb on - strand, within GFF591 at 638.305 kb on + strand, within GFF591 at 638.528 kb on + strand, within GFF591 at 638.529 kb on - strand, within GFF591 at 638.978 kb on + strand, within GFF592 at 638.978 kb on + strand, within GFF592 at 638.978 kb on + strand, within GFF592 at 638.978 kb on + strand, within GFF592 at 638.979 kb on - strand, within GFF592 at 638.979 kb on - strand, within GFF592 at 639.104 kb on + strand, within GFF592 at 639.284 kb on + strand, within GFF592 at 639.284 kb on + strand, within GFF592 at 639.285 kb on - strand, within GFF592 at 639.285 kb on - strand, within GFF592 at 639.285 kb on - strand, within GFF592 at 639.413 kb on + strand, within GFF592 at 639.413 kb on + strand, within GFF592 at 639.413 kb on + strand, within GFF592 at 639.414 kb on - strand, within GFF592 at 639.414 kb on - strand, within GFF592 at 639.414 kb on - strand, within GFF592 at 639.614 kb on + strand at 639.615 kb on - strand at 639.630 kb on - strand at 639.630 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 636,678 - GFF590 0.13 -0.4 637,094 + GFF590 0.57 -0.6 637,095 - GFF590 0.57 +0.1 637,095 - GFF590 0.57 -1.3 637,328 + GFF590 0.82 -1.7 637,328 + GFF590 0.82 +1.2 637,328 + GFF590 0.82 -0.6 637,329 - GFF590 0.82 -1.8 637,329 - GFF590 0.82 -0.6 637,329 - GFF590 0.82 -0.1 637,530 + +0.9 637,567 - +1.2 637,570 + +2.2 637,570 + +0.2 637,570 + -0.7 637,570 + +1.2 637,570 + -1.4 637,570 + -1.1 637,570 + -1.2 637,570 + -0.8 637,570 + -1.2 637,570 + -0.2 637,571 - +0.4 637,571 - -1.4 637,571 - +1.0 637,571 - +0.1 637,571 - +1.2 637,571 - +0.3 637,571 - -0.8 637,702 + -2.2 637,702 + -0.1 637,702 + -1.6 637,702 + +0.5 637,702 + +0.6 637,702 + +1.8 637,703 - +0.3 637,703 - -0.6 637,703 - +0.6 637,703 - -0.6 637,858 + GFF591 0.22 -0.4 637,858 + GFF591 0.22 -0.0 637,859 - GFF591 0.22 +0.2 638,164 + GFF591 0.49 -1.5 638,164 + GFF591 0.49 +0.6 638,165 - GFF591 0.49 -1.7 638,165 - GFF591 0.49 +1.2 638,165 - GFF591 0.49 -1.4 638,305 + GFF591 0.62 -0.3 638,528 + GFF591 0.82 +0.2 638,529 - GFF591 0.82 -0.1 638,978 + GFF592 0.28 -0.0 638,978 + GFF592 0.28 -0.3 638,978 + GFF592 0.28 -0.2 638,978 + GFF592 0.28 -0.4 638,979 - GFF592 0.28 +0.6 638,979 - GFF592 0.28 -0.4 639,104 + GFF592 0.42 +0.0 639,284 + GFF592 0.61 -0.4 639,284 + GFF592 0.61 +0.2 639,285 - GFF592 0.62 -0.1 639,285 - GFF592 0.62 -0.2 639,285 - GFF592 0.62 +0.4 639,413 + GFF592 0.76 -0.5 639,413 + GFF592 0.76 -2.1 639,413 + GFF592 0.76 -0.2 639,414 - GFF592 0.76 +0.0 639,414 - GFF592 0.76 +0.0 639,414 - GFF592 0.76 -0.6 639,614 + -0.6 639,615 - +0.5 639,630 - +1.3 639,630 - -1.6
Or see this region's nucleotide sequence