Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5825 and GFF5826 are separated by 10 nucleotides GFF5826 and GFF5827 overlap by 4 nucleotides GFF5827 and GFF5828 overlap by 4 nucleotides GFF5828 and GFF5829 are separated by 12 nucleotides
GFF5825 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 1,559 to 2,266
GFF5825
GFF5826 - Branched-chain amino acid transport ATP-binding protein LivG (TC 3.A.1.4.1), at 2,277 to 2,984
GFF5826
GFF5827 - FIG00452956: hypothetical protein, at 2,981 to 4,054
GFF5827
GFF5828 - Branched-chain amino acid ABC transporter, permease protein LivH (TC 3.A.1.4.1), at 4,051 to 4,920
GFF5828
GFF5829 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 4,933 to 6,201
GFF5829
Position (kb) 2
3
4
5 Strain fitness (log2 ratio) -2
-1
0
1 at 1.994 kb on + strand, within GFF5825 at 1.994 kb on + strand, within GFF5825 at 2.294 kb on - strand at 2.294 kb on - strand at 3.120 kb on + strand, within GFF5827 at 3.121 kb on - strand, within GFF5827 at 3.121 kb on - strand, within GFF5827 at 3.201 kb on + strand, within GFF5827 at 3.201 kb on + strand, within GFF5827 at 3.201 kb on + strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.202 kb on - strand, within GFF5827 at 3.489 kb on + strand, within GFF5827 at 3.490 kb on - strand, within GFF5827 at 3.490 kb on - strand, within GFF5827 at 3.490 kb on - strand, within GFF5827 at 3.645 kb on + strand, within GFF5827 at 3.645 kb on + strand, within GFF5827 at 3.645 kb on + strand, within GFF5827 at 3.646 kb on - strand, within GFF5827 at 3.646 kb on - strand, within GFF5827 at 3.795 kb on + strand, within GFF5827 at 3.795 kb on + strand, within GFF5827 at 4.306 kb on + strand, within GFF5828 at 4.306 kb on + strand, within GFF5828 at 4.307 kb on - strand, within GFF5828 at 4.586 kb on + strand, within GFF5828 at 4.778 kb on + strand, within GFF5828 at 4.778 kb on + strand, within GFF5828 at 4.778 kb on + strand, within GFF5828 at 4.778 kb on + strand, within GFF5828 at 4.835 kb on + strand at 4.835 kb on + strand at 4.869 kb on - strand at 4.910 kb on + strand at 4.934 kb on + strand at 4.935 kb on - strand at 4.935 kb on - strand at 4.935 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 1,994 + GFF5825 0.61 -0.0 1,994 + GFF5825 0.61 +0.1 2,294 - -0.2 2,294 - +0.5 3,120 + GFF5827 0.13 -0.1 3,121 - GFF5827 0.13 +0.6 3,121 - GFF5827 0.13 +0.0 3,201 + GFF5827 0.20 +0.7 3,201 + GFF5827 0.20 +0.9 3,201 + GFF5827 0.20 -0.0 3,202 - GFF5827 0.21 +0.7 3,202 - GFF5827 0.21 +0.5 3,202 - GFF5827 0.21 -0.8 3,202 - GFF5827 0.21 -0.6 3,202 - GFF5827 0.21 -0.4 3,202 - GFF5827 0.21 -0.1 3,489 + GFF5827 0.47 -0.1 3,490 - GFF5827 0.47 +0.2 3,490 - GFF5827 0.47 -0.1 3,490 - GFF5827 0.47 +0.4 3,645 + GFF5827 0.62 -0.0 3,645 + GFF5827 0.62 -0.3 3,645 + GFF5827 0.62 -0.3 3,646 - GFF5827 0.62 +1.0 3,646 - GFF5827 0.62 -0.5 3,795 + GFF5827 0.76 -0.3 3,795 + GFF5827 0.76 +0.5 4,306 + GFF5828 0.29 +0.8 4,306 + GFF5828 0.29 +1.1 4,307 - GFF5828 0.29 +0.4 4,586 + GFF5828 0.61 -0.2 4,778 + GFF5828 0.84 +0.3 4,778 + GFF5828 0.84 +0.1 4,778 + GFF5828 0.84 -0.3 4,778 + GFF5828 0.84 +1.4 4,835 + -0.1 4,835 + +0.2 4,869 - -0.1 4,910 + -1.0 4,934 + +0.3 4,935 - -1.8 4,935 - -0.1 4,935 - -0.2
Or see this region's nucleotide sequence