Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5701 and GFF5702 overlap by 4 nucleotides GFF5702 and GFF5703 overlap by 4 nucleotides GFF5703 and GFF5704 are separated by 23 nucleotides
GFF5701 - FKBP-type peptidyl-prolyl cis-trans isomerase SlpA (EC 5.2.1.8), at 75,499 to 75,942
GFF5701
GFF5702 - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (EC 1.17.7.4), at 75,939 to 76,907
GFF5702
GFF5703 - pyridoxal-phosphate dependent enzyme family protein, at 76,904 to 77,869
GFF5703
GFF5704 - Seryl-tRNA synthetase (EC 6.1.1.11), at 77,893 to 79,224
GFF5704
Position (kb)
76
77
78 Strain fitness (log2 ratio)
-2
-1
0
1 at 75.938 kb on + strand at 75.938 kb on + strand at 75.938 kb on + strand at 75.938 kb on + strand at 75.938 kb on + strand at 75.938 kb on + strand at 75.939 kb on - strand at 75.939 kb on - strand at 75.939 kb on - strand at 75.939 kb on - strand at 75.939 kb on - strand at 75.939 kb on - strand at 76.941 kb on + strand at 76.942 kb on - strand at 76.945 kb on + strand at 76.945 kb on + strand at 76.945 kb on + strand at 76.945 kb on + strand at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.306 kb on + strand, within GFF5703 at 77.307 kb on - strand, within GFF5703 at 77.372 kb on + strand, within GFF5703 at 77.372 kb on + strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.373 kb on - strand, within GFF5703 at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.795 kb on + strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.796 kb on - strand at 77.881 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 75,938 + +0.3 75,938 + -0.7 75,938 + -1.0 75,938 + +1.1 75,938 + -1.0 75,938 + -0.6 75,939 - -0.7 75,939 - +0.6 75,939 - +0.5 75,939 - +0.2 75,939 - +0.5 75,939 - +0.3 76,941 + -0.2 76,942 - -0.7 76,945 + -0.7 76,945 + -1.7 76,945 + -0.4 76,945 + -1.2 77,306 + GFF5703 0.42 +0.1 77,306 + GFF5703 0.42 -0.9 77,306 + GFF5703 0.42 -1.0 77,306 + GFF5703 0.42 +0.1 77,306 + GFF5703 0.42 -0.7 77,307 - GFF5703 0.42 -0.0 77,372 + GFF5703 0.48 -1.9 77,372 + GFF5703 0.48 -1.2 77,373 - GFF5703 0.49 -0.6 77,373 - GFF5703 0.49 -0.4 77,373 - GFF5703 0.49 -0.5 77,373 - GFF5703 0.49 -0.5 77,373 - GFF5703 0.49 -0.6 77,795 + +0.5 77,795 + -0.9 77,795 + +0.4 77,795 + -0.4 77,795 + -0.6 77,795 + -1.9 77,796 - -0.5 77,796 - +0.7 77,796 - -1.1 77,796 - -0.5 77,796 - -1.4 77,796 - -0.3 77,796 - +0.7 77,796 - +0.3 77,881 + -0.7
Or see this region's nucleotide sequence