Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5651 and GFF5652 overlap by 35 nucleotides GFF5652 and GFF5653 are separated by 14 nucleotides GFF5653 and GFF5654 are separated by 34 nucleotides
GFF5651 - hypothetical protein, at 26,909 to 28,111
GFF5651
GFF5652 - FIG00014764: hypothetical protein, at 28,077 to 28,940
GFF5652
GFF5653 - Acyl-CoA dehydrogenase (EC 1.3.8.1), Mycobacterial subgroup FadE16, at 28,955 to 30,070
GFF5653
GFF5654 - L-seryl-tRNA(Sec) selenium transferase (EC 2.9.1.1), at 30,105 to 31,553
GFF5654
Position (kb) 28
29
30
31 Strain fitness (log2 ratio) -1
0
1 at 28.083 kb on + strand at 28.121 kb on - strand at 28.319 kb on - strand, within GFF5652 at 28.319 kb on - strand, within GFF5652 at 28.319 kb on - strand, within GFF5652 at 28.319 kb on - strand, within GFF5652 at 28.810 kb on + strand, within GFF5652 at 28.811 kb on - strand, within GFF5652 at 28.820 kb on - strand, within GFF5652 at 28.820 kb on - strand, within GFF5652 at 28.828 kb on + strand, within GFF5652 at 28.828 kb on + strand, within GFF5652 at 28.829 kb on - strand, within GFF5652 at 28.829 kb on - strand, within GFF5652 at 28.900 kb on + strand at 28.998 kb on + strand at 28.998 kb on + strand at 28.998 kb on + strand at 28.999 kb on - strand at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.616 kb on + strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.617 kb on - strand, within GFF5653 at 29.826 kb on + strand, within GFF5653 at 29.826 kb on + strand, within GFF5653 at 29.827 kb on - strand, within GFF5653 at 29.827 kb on - strand, within GFF5653 at 30.107 kb on - strand at 30.107 kb on - strand at 30.506 kb on - strand, within GFF5654 at 30.506 kb on - strand, within GFF5654 at 30.506 kb on - strand, within GFF5654 at 30.506 kb on - strand, within GFF5654 at 30.718 kb on + strand, within GFF5654 at 30.718 kb on + strand, within GFF5654 at 30.718 kb on + strand, within GFF5654 at 30.719 kb on - strand, within GFF5654 at 30.719 kb on - strand, within GFF5654 at 30.719 kb on - strand, within GFF5654 at 30.838 kb on + strand, within GFF5654 at 30.838 kb on + strand, within GFF5654 at 30.838 kb on + strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.839 kb on - strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.895 kb on + strand, within GFF5654 at 30.896 kb on - strand, within GFF5654 at 30.896 kb on - strand, within GFF5654
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 28,083 + -1.2 28,121 - +0.1 28,319 - GFF5652 0.28 -0.2 28,319 - GFF5652 0.28 +1.1 28,319 - GFF5652 0.28 +1.6 28,319 - GFF5652 0.28 +0.1 28,810 + GFF5652 0.85 -0.7 28,811 - GFF5652 0.85 +0.1 28,820 - GFF5652 0.86 -0.0 28,820 - GFF5652 0.86 -0.7 28,828 + GFF5652 0.87 -0.9 28,828 + GFF5652 0.87 -0.6 28,829 - GFF5652 0.87 -0.4 28,829 - GFF5652 0.87 +0.3 28,900 + -0.3 28,998 + -1.2 28,998 + -0.0 28,998 + -0.4 28,999 - -0.6 29,616 + GFF5653 0.59 -1.2 29,616 + GFF5653 0.59 -0.5 29,616 + GFF5653 0.59 -0.6 29,616 + GFF5653 0.59 -0.6 29,616 + GFF5653 0.59 -0.3 29,616 + GFF5653 0.59 +0.1 29,616 + GFF5653 0.59 -0.8 29,616 + GFF5653 0.59 -0.8 29,616 + GFF5653 0.59 +0.6 29,617 - GFF5653 0.59 +0.3 29,617 - GFF5653 0.59 +0.3 29,617 - GFF5653 0.59 -0.0 29,617 - GFF5653 0.59 -1.0 29,617 - GFF5653 0.59 -0.8 29,617 - GFF5653 0.59 -0.2 29,617 - GFF5653 0.59 -0.7 29,617 - GFF5653 0.59 -0.4 29,617 - GFF5653 0.59 -0.5 29,617 - GFF5653 0.59 -0.7 29,617 - GFF5653 0.59 +0.4 29,617 - GFF5653 0.59 -1.0 29,617 - GFF5653 0.59 -0.4 29,617 - GFF5653 0.59 +0.2 29,617 - GFF5653 0.59 +1.3 29,617 - GFF5653 0.59 -0.5 29,617 - GFF5653 0.59 -0.9 29,826 + GFF5653 0.78 -0.6 29,826 + GFF5653 0.78 -0.7 29,827 - GFF5653 0.78 -0.3 29,827 - GFF5653 0.78 -0.7 30,107 - -1.0 30,107 - +0.2 30,506 - GFF5654 0.28 +0.0 30,506 - GFF5654 0.28 -0.3 30,506 - GFF5654 0.28 -0.8 30,506 - GFF5654 0.28 +0.6 30,718 + GFF5654 0.42 -0.6 30,718 + GFF5654 0.42 +0.4 30,718 + GFF5654 0.42 -1.2 30,719 - GFF5654 0.42 +0.3 30,719 - GFF5654 0.42 -1.4 30,719 - GFF5654 0.42 +0.3 30,838 + GFF5654 0.51 -0.3 30,838 + GFF5654 0.51 -1.0 30,838 + GFF5654 0.51 -0.2 30,839 - GFF5654 0.51 +0.1 30,839 - GFF5654 0.51 -0.1 30,839 - GFF5654 0.51 +0.1 30,839 - GFF5654 0.51 -1.5 30,895 + GFF5654 0.55 +1.2 30,895 + GFF5654 0.55 +0.3 30,895 + GFF5654 0.55 -0.7 30,895 + GFF5654 0.55 -0.9 30,895 + GFF5654 0.55 -0.2 30,896 - GFF5654 0.55 -0.5 30,896 - GFF5654 0.55 +0.1
Or see this region's nucleotide sequence