Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5650 and GFF5651 are separated by 13 nucleotides GFF5651 and GFF5652 overlap by 35 nucleotides GFF5652 and GFF5653 are separated by 14 nucleotides
GFF5650 - Selenocysteine-specific translation elongation factor, at 24,988 to 26,895
GFF5650
GFF5651 - hypothetical protein, at 26,909 to 28,111
GFF5651
GFF5652 - FIG00014764: hypothetical protein, at 28,077 to 28,940
GFF5652
GFF5653 - Acyl-CoA dehydrogenase (EC 1.3.8.1), Mycobacterial subgroup FadE16, at 28,955 to 30,070
GFF5653
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 26.602 kb on + strand, within GFF5650 at 26.603 kb on - strand, within GFF5650 at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.765 kb on + strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.766 kb on - strand at 26.771 kb on + strand at 26.772 kb on - strand at 26.870 kb on - strand at 27.172 kb on - strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.585 kb on + strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 27.586 kb on - strand, within GFF5651 at 28.083 kb on + strand at 28.121 kb on - strand at 28.319 kb on - strand, within GFF5652 at 28.319 kb on - strand, within GFF5652 at 28.319 kb on - strand, within GFF5652 at 28.319 kb on - strand, within GFF5652 at 28.810 kb on + strand, within GFF5652 at 28.811 kb on - strand, within GFF5652 at 28.820 kb on - strand, within GFF5652 at 28.820 kb on - strand, within GFF5652 at 28.828 kb on + strand, within GFF5652 at 28.828 kb on + strand, within GFF5652 at 28.829 kb on - strand, within GFF5652 at 28.829 kb on - strand, within GFF5652 at 28.900 kb on + strand at 28.998 kb on + strand at 28.998 kb on + strand at 28.998 kb on + strand at 28.999 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 26,602 + GFF5650 0.85 -0.3 26,603 - GFF5650 0.85 -0.9 26,765 + +0.5 26,765 + -1.2 26,765 + +0.1 26,765 + -1.3 26,765 + +0.4 26,766 - -0.1 26,766 - -0.0 26,766 - -0.7 26,766 - -1.4 26,766 - -0.1 26,771 + -0.1 26,772 - +0.1 26,870 - -0.0 27,172 - GFF5651 0.22 -0.4 27,585 + GFF5651 0.56 -0.6 27,585 + GFF5651 0.56 -2.8 27,585 + GFF5651 0.56 -2.3 27,585 + GFF5651 0.56 -0.5 27,585 + GFF5651 0.56 -0.3 27,585 + GFF5651 0.56 -0.1 27,585 + GFF5651 0.56 +0.6 27,585 + GFF5651 0.56 -2.0 27,585 + GFF5651 0.56 -0.3 27,586 - GFF5651 0.56 -1.4 27,586 - GFF5651 0.56 -0.4 27,586 - GFF5651 0.56 -0.4 27,586 - GFF5651 0.56 -0.2 27,586 - GFF5651 0.56 -1.0 28,083 + -1.2 28,121 - +0.1 28,319 - GFF5652 0.28 -0.2 28,319 - GFF5652 0.28 +1.1 28,319 - GFF5652 0.28 +1.6 28,319 - GFF5652 0.28 +0.1 28,810 + GFF5652 0.85 -0.7 28,811 - GFF5652 0.85 +0.1 28,820 - GFF5652 0.86 -0.0 28,820 - GFF5652 0.86 -0.7 28,828 + GFF5652 0.87 -0.9 28,828 + GFF5652 0.87 -0.6 28,829 - GFF5652 0.87 -0.4 28,829 - GFF5652 0.87 +0.3 28,900 + -0.3 28,998 + -1.2 28,998 + -0.0 28,998 + -0.4 28,999 - -0.6
Or see this region's nucleotide sequence