Strain Fitness in Variovorax sp. SCN45 around GFF5627

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
4,506	+		GFF5626	0.60	-0.6
4,506	+		GFF5626	0.60	-0.5
4,506	+		GFF5626	0.60	+0.5
4,506	+		GFF5626	0.60	-0.8
4,506	+		GFF5626	0.60	+0.2
4,506	+		GFF5626	0.60	+1.0
4,507	-		GFF5626	0.60	+0.2
4,507	-		GFF5626	0.60	-0.4
4,725	+		GFF5626	0.78	-0.4
4,725	+		GFF5626	0.78	+0.4
4,725	+		GFF5626	0.78	+0.1
4,725	+		GFF5626	0.78	-1.4
4,725	+		GFF5626	0.78	-0.3
4,725	+		GFF5626	0.78	-0.3
4,725	+		GFF5626	0.78	+0.4
4,725	+		GFF5626	0.78	-0.3
4,725	+		GFF5626	0.78	-1.0
4,726	-		GFF5626	0.78	+0.5
4,726	-		GFF5626	0.78	-0.8
4,929	+				+0.7
4,929	+				+0.8
4,929	+				-0.2
4,929	+				+0.0
4,930	-				-0.6
4,930	-				-1.0
4,999	+				-0.2
5,037	+				+0.9
5,037	+				+1.2
5,037	+				-0.1
5,038	-				-0.2
5,038	-				+0.3
5,038	-				-1.4
5,038	-				+0.3
5,038	-				-0.2
5,038	-				+0.7
5,038	-				+0.7
5,038	-				+0.1
5,043	-				+0.2
5,120	+				-0.6
5,478	+		GFF5627	0.38	-1.2
5,478	+		GFF5627	0.38	-2.1
5,478	+		GFF5627	0.38	-1.6
5,478	+		GFF5627	0.38	-1.5
5,479	-		GFF5627	0.38	-3.7
5,479	-		GFF5627	0.38	-0.2
5,479	-		GFF5627	0.38	-1.0
5,479	-		GFF5627	0.38	-0.9
5,479	-		GFF5627	0.38	+1.0
5,479	-		GFF5627	0.38	-0.8
5,479	-		GFF5627	0.38	-1.8
5,479	-		GFF5627	0.38	-1.0
5,479	-		GFF5627	0.38	-1.9
5,479	-		GFF5627	0.38	-2.2
5,688	+		GFF5627	0.57	-1.9
5,688	+		GFF5627	0.57	-2.6
5,688	+		GFF5627	0.57	-0.2
5,689	-		GFF5627	0.57	-0.4
5,961	+		GFF5627	0.82	+2.2
5,961	+		GFF5627	0.82	+0.0
5,961	+		GFF5627	0.82	-1.0
5,961	+		GFF5627	0.82	-2.2
5,962	-		GFF5627	0.82	-2.3
6,060	+				-1.3
6,060	+				-0.6
6,060	+				-0.5
6,060	+				-1.2
6,060	+				-2.3
6,060	+				-2.0
6,060	+				-0.9
6,060	+				+1.6
6,060	+				-0.4
6,060	+				-0.2
6,060	+				+0.3
6,060	+				-1.2
6,060	+				-1.7
6,060	+				-1.7
6,061	-				-1.2
6,061	-				-2.7
6,061	-				-1.9
6,061	-				-0.7
6,061	-				-0.1
6,156	+				-1.1
6,156	+				-0.1
6,156	+				+0.2
6,156	+				+0.6
6,156	+				-0.4
6,156	+				-0.3
6,156	+				+0.3
6,156	+				+1.1
6,156	+				-0.2
6,156	+				+1.0
6,157	-				-0.6
6,157	-				-1.5
6,157	-				+0.2
6,157	-				-0.2
6,157	-				-0.8
6,157	-				-0.5
6,157	-				+0.0
6,157	-				-1.0
6,157	-				-0.2
6,157	-				-0.7
6,157	-				-0.2
6,157	-				-1.2
6,185	+				-0.9
6,185	+				-0.7
6,185	+				-0.4
6,186	-				+0.9
6,186	-				-0.6
6,186	-				-1.7
6,186	-				-0.1
6,186	-				-0.1
6,186	-				-1.2
6,186	-				-1.3
6,323	+		GFF5628	0.17	-0.5
6,323	+		GFF5628	0.17	-0.2
6,323	+		GFF5628	0.17	+0.5
6,323	+		GFF5628	0.17	+0.0
6,323	+		GFF5628	0.17	-0.9
6,324	-		GFF5628	0.17	-0.5
6,324	-		GFF5628	0.17	+0.0
6,324	-		GFF5628	0.17	-0.5
6,324	-		GFF5628	0.17	-0.7
6,324	-		GFF5628	0.17	+0.3
6,324	-		GFF5628	0.17	-0.8
6,599	+		GFF5628	0.46	-0.4
6,600	-		GFF5628	0.46	-0.5
6,662	+		GFF5628	0.53	-0.4
6,662	+		GFF5628	0.53	-0.2
6,662	+		GFF5628	0.53	+0.0
6,662	+		GFF5628	0.53	-1.0
6,662	+		GFF5628	0.53	-0.5
6,662	+		GFF5628	0.53	-0.1
6,662	+		GFF5628	0.53	-1.5
6,663	-		GFF5628	0.53	-0.5
6,663	-		GFF5628	0.53	+0.5
6,663	-		GFF5628	0.53	+0.2
6,663	-		GFF5628	0.53	+0.3
6,663	-		GFF5628	0.53	+0.1
7,106	+				-0.5
7,106	+				+1.6