Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5346 and GFF5347 are separated by 10 nucleotides GFF5347 and GFF5348 overlap by 4 nucleotides GFF5348 and GFF5349 overlap by 4 nucleotides GFF5349 and GFF5350 are separated by 19 nucleotides
GFF5346 - Putative outer membrane lipoprotein, at 224,775 to 225,587
GFF5346
GFF5347 - hypothetical protein, at 225,598 to 225,948
GFF5347
GFF5348 - Histidinol-phosphate aminotransferase (EC 2.6.1.9), at 225,945 to 227,036
GFF5348
GFF5349 - Imidazoleglycerol-phosphate dehydratase (EC 4.2.1.19), at 227,033 to 227,650
GFF5349
GFF5350 - Imidazole glycerol phosphate synthase amidotransferase subunit HisH, at 227,670 to 228,326
GFF5350
Position (kb)
225
226
227
228 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 225.015 kb on + strand, within GFF5346 at 225.015 kb on + strand, within GFF5346 at 225.016 kb on - strand, within GFF5346 at 225.016 kb on - strand, within GFF5346 at 225.016 kb on - strand, within GFF5346 at 225.208 kb on - strand, within GFF5346 at 225.208 kb on - strand, within GFF5346 at 225.243 kb on + strand, within GFF5346 at 225.243 kb on + strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.244 kb on - strand, within GFF5346 at 225.287 kb on + strand, within GFF5346 at 225.287 kb on + strand, within GFF5346 at 225.287 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.315 kb on + strand, within GFF5346 at 225.316 kb on - strand, within GFF5346 at 225.316 kb on - strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.411 kb on + strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.412 kb on - strand, within GFF5346 at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.558 kb on + strand at 225.559 kb on - strand at 225.559 kb on - strand at 225.559 kb on - strand at 225.559 kb on - strand at 225.712 kb on + strand, within GFF5347 at 225.712 kb on + strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.713 kb on - strand, within GFF5347 at 225.721 kb on + strand, within GFF5347 at 225.721 kb on + strand, within GFF5347 at 225.721 kb on + strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.722 kb on - strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.724 kb on + strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.725 kb on - strand, within GFF5347 at 225.844 kb on + strand, within GFF5347 at 225.844 kb on + strand, within GFF5347 at 225.844 kb on + strand, within GFF5347 at 225.845 kb on - strand, within GFF5347 at 225.877 kb on + strand, within GFF5347 at 225.877 kb on + strand, within GFF5347 at 225.878 kb on - strand, within GFF5347 at 225.878 kb on - strand, within GFF5347 at 225.878 kb on - strand, within GFF5347 at 225.878 kb on - strand, within GFF5347 at 225.878 kb on - strand, within GFF5347 at 225.931 kb on + strand at 225.931 kb on + strand at 225.931 kb on + strand at 225.932 kb on - strand at 225.932 kb on - strand at 225.932 kb on - strand at 225.932 kb on - strand at 225.932 kb on - strand at 225.932 kb on - strand at 225.932 kb on - strand at 225.997 kb on + strand at 225.997 kb on + strand at 225.997 kb on + strand at 225.997 kb on + strand at 225.997 kb on + strand at 225.997 kb on + strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.998 kb on - strand at 225.999 kb on - strand at 226.014 kb on + strand at 226.014 kb on + strand at 226.014 kb on + strand at 226.015 kb on - strand at 226.015 kb on - strand at 226.015 kb on - strand at 226.015 kb on - strand at 226.015 kb on - strand at 226.015 kb on - strand at 226.021 kb on + strand at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.308 kb on + strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.309 kb on - strand, within GFF5348 at 226.428 kb on + strand, within GFF5348 at 226.428 kb on + strand, within GFF5348 at 226.429 kb on - strand, within GFF5348 at 226.438 kb on - strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.539 kb on + strand, within GFF5348 at 226.540 kb on - strand, within GFF5348 at 226.540 kb on - strand, within GFF5348 at 226.540 kb on - strand, within GFF5348 at 226.540 kb on - strand, within GFF5348 at 226.540 kb on - strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.563 kb on + strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.564 kb on - strand, within GFF5348 at 226.647 kb on + strand, within GFF5348 at 226.647 kb on + strand, within GFF5348 at 226.648 kb on - strand, within GFF5348 at 226.648 kb on - strand, within GFF5348 at 226.702 kb on - strand, within GFF5348 at 226.702 kb on - strand, within GFF5348 at 226.702 kb on - strand, within GFF5348 at 226.702 kb on - strand, within GFF5348 at 226.702 kb on - strand, within GFF5348 at 226.702 kb on - strand, within GFF5348 at 226.940 kb on + strand at 226.940 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.032 kb on + strand at 227.033 kb on - strand at 227.033 kb on - strand at 227.669 kb on + strand at 227.669 kb on + strand at 227.670 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 225,015 + GFF5346 0.30 -1.1 225,015 + GFF5346 0.30 -0.6 225,016 - GFF5346 0.30 -0.5 225,016 - GFF5346 0.30 -0.3 225,016 - GFF5346 0.30 -0.0 225,208 - GFF5346 0.53 +0.4 225,208 - GFF5346 0.53 +0.1 225,243 + GFF5346 0.58 -2.6 225,243 + GFF5346 0.58 -0.5 225,244 - GFF5346 0.58 +0.3 225,244 - GFF5346 0.58 -0.9 225,244 - GFF5346 0.58 -0.4 225,244 - GFF5346 0.58 -1.0 225,244 - GFF5346 0.58 -0.5 225,244 - GFF5346 0.58 -0.0 225,287 + GFF5346 0.63 +0.0 225,287 + GFF5346 0.63 +1.4 225,287 + GFF5346 0.63 +0.6 225,315 + GFF5346 0.66 -0.2 225,315 + GFF5346 0.66 -1.5 225,315 + GFF5346 0.66 +0.1 225,315 + GFF5346 0.66 -0.5 225,316 - GFF5346 0.67 +1.1 225,316 - GFF5346 0.67 +0.0 225,411 + GFF5346 0.78 +0.6 225,411 + GFF5346 0.78 +0.5 225,411 + GFF5346 0.78 -0.3 225,411 + GFF5346 0.78 +0.4 225,412 - GFF5346 0.78 +1.0 225,412 - GFF5346 0.78 -0.4 225,412 - GFF5346 0.78 -0.2 225,412 - GFF5346 0.78 +0.3 225,412 - GFF5346 0.78 +0.2 225,558 + +0.3 225,558 + +0.6 225,558 + +0.0 225,558 + -1.7 225,558 + +2.2 225,558 + -0.8 225,559 - -1.0 225,559 - +1.8 225,559 - -0.3 225,559 - +0.4 225,712 + GFF5347 0.32 +0.9 225,712 + GFF5347 0.32 -1.2 225,713 - GFF5347 0.33 -0.2 225,713 - GFF5347 0.33 +0.3 225,713 - GFF5347 0.33 -1.5 225,713 - GFF5347 0.33 +0.6 225,713 - GFF5347 0.33 -1.5 225,713 - GFF5347 0.33 -0.0 225,721 + GFF5347 0.35 +1.6 225,721 + GFF5347 0.35 -0.0 225,721 + GFF5347 0.35 -0.7 225,722 - GFF5347 0.35 -0.0 225,722 - GFF5347 0.35 -0.2 225,722 - GFF5347 0.35 +1.6 225,722 - GFF5347 0.35 -0.4 225,722 - GFF5347 0.35 -1.4 225,724 + GFF5347 0.36 +0.4 225,724 + GFF5347 0.36 -1.7 225,724 + GFF5347 0.36 +0.6 225,724 + GFF5347 0.36 -0.1 225,724 + GFF5347 0.36 +0.4 225,724 + GFF5347 0.36 -0.5 225,724 + GFF5347 0.36 -0.7 225,724 + GFF5347 0.36 -0.8 225,725 - GFF5347 0.36 +0.8 225,725 - GFF5347 0.36 +0.2 225,725 - GFF5347 0.36 +0.4 225,725 - GFF5347 0.36 -1.4 225,725 - GFF5347 0.36 -0.4 225,725 - GFF5347 0.36 +0.3 225,725 - GFF5347 0.36 -1.4 225,844 + GFF5347 0.70 -2.4 225,844 + GFF5347 0.70 +1.6 225,844 + GFF5347 0.70 +0.8 225,845 - GFF5347 0.70 -0.4 225,877 + GFF5347 0.79 -1.8 225,877 + GFF5347 0.79 -1.2 225,878 - GFF5347 0.80 -0.0 225,878 - GFF5347 0.80 +0.6 225,878 - GFF5347 0.80 +1.6 225,878 - GFF5347 0.80 -1.9 225,878 - GFF5347 0.80 -0.3 225,931 + -0.7 225,931 + -1.8 225,931 + -0.8 225,932 - -0.1 225,932 - +0.1 225,932 - -0.3 225,932 - +2.9 225,932 - -1.6 225,932 - -0.3 225,932 - -0.3 225,997 + -0.9 225,997 + -0.7 225,997 + +0.2 225,997 + -0.8 225,997 + +1.0 225,997 + -0.6 225,998 - -0.6 225,998 - +0.2 225,998 - +0.6 225,998 - -0.7 225,998 - +0.0 225,998 - -0.3 225,998 - -0.5 225,998 - +0.8 225,999 - +0.1 226,014 + +0.6 226,014 + -0.0 226,014 + -0.1 226,015 - +1.3 226,015 - -1.7 226,015 - -0.5 226,015 - -0.3 226,015 - -0.9 226,015 - +0.0 226,021 + -1.2 226,308 + GFF5348 0.33 +0.2 226,308 + GFF5348 0.33 -0.4 226,308 + GFF5348 0.33 +1.4 226,308 + GFF5348 0.33 -0.6 226,308 + GFF5348 0.33 +0.9 226,308 + GFF5348 0.33 -1.4 226,308 + GFF5348 0.33 +0.1 226,308 + GFF5348 0.33 -1.9 226,308 + GFF5348 0.33 +0.2 226,308 + GFF5348 0.33 -0.4 226,308 + GFF5348 0.33 +0.6 226,308 + GFF5348 0.33 +0.1 226,308 + GFF5348 0.33 +0.1 226,308 + GFF5348 0.33 -0.6 226,308 + GFF5348 0.33 -0.4 226,308 + GFF5348 0.33 -0.7 226,309 - GFF5348 0.33 -1.2 226,309 - GFF5348 0.33 -1.0 226,309 - GFF5348 0.33 -1.0 226,309 - GFF5348 0.33 +1.1 226,309 - GFF5348 0.33 +0.1 226,309 - GFF5348 0.33 +1.0 226,309 - GFF5348 0.33 -0.7 226,309 - GFF5348 0.33 +0.4 226,309 - GFF5348 0.33 -0.0 226,309 - GFF5348 0.33 -0.8 226,309 - GFF5348 0.33 +0.2 226,309 - GFF5348 0.33 +0.6 226,309 - GFF5348 0.33 +0.6 226,428 + GFF5348 0.44 +0.8 226,428 + GFF5348 0.44 +0.6 226,429 - GFF5348 0.44 +0.6 226,438 - GFF5348 0.45 -0.5 226,539 + GFF5348 0.54 -0.1 226,539 + GFF5348 0.54 -0.8 226,539 + GFF5348 0.54 +0.5 226,539 + GFF5348 0.54 +0.1 226,539 + GFF5348 0.54 +0.4 226,539 + GFF5348 0.54 +0.2 226,539 + GFF5348 0.54 -0.0 226,539 + GFF5348 0.54 -0.2 226,540 - GFF5348 0.54 +0.3 226,540 - GFF5348 0.54 -1.4 226,540 - GFF5348 0.54 -0.2 226,540 - GFF5348 0.54 +0.5 226,540 - GFF5348 0.54 -0.4 226,563 + GFF5348 0.57 -0.4 226,563 + GFF5348 0.57 -0.2 226,563 + GFF5348 0.57 +0.3 226,563 + GFF5348 0.57 -0.4 226,563 + GFF5348 0.57 +0.3 226,563 + GFF5348 0.57 -0.6 226,563 + GFF5348 0.57 +0.3 226,563 + GFF5348 0.57 +1.1 226,564 - GFF5348 0.57 +0.6 226,564 - GFF5348 0.57 +0.1 226,564 - GFF5348 0.57 -0.4 226,564 - GFF5348 0.57 -1.5 226,564 - GFF5348 0.57 +0.3 226,564 - GFF5348 0.57 -0.1 226,564 - GFF5348 0.57 +1.9 226,647 + GFF5348 0.64 -1.7 226,647 + GFF5348 0.64 -1.3 226,648 - GFF5348 0.64 -0.2 226,648 - GFF5348 0.64 +0.3 226,702 - GFF5348 0.69 -0.7 226,702 - GFF5348 0.69 -0.7 226,702 - GFF5348 0.69 +0.1 226,702 - GFF5348 0.69 -0.5 226,702 - GFF5348 0.69 -0.1 226,702 - GFF5348 0.69 -0.3 226,940 + +0.7 226,940 + +0.6 227,032 + -2.3 227,032 + -0.9 227,032 + +1.2 227,032 + -0.5 227,032 + -0.6 227,032 + +0.3 227,032 + +0.2 227,032 + -0.3 227,033 - +0.4 227,033 - -0.8 227,669 + -0.5 227,669 + -2.0 227,670 - +0.7
Or see this region's nucleotide sequence