Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5261 and GFF5262 are separated by 144 nucleotides GFF5262 and GFF5263 are separated by 0 nucleotides
GFF5261 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 126,360 to 127,832
GFF5261
GFF5262 - Ferric iron ABC transporter, iron-binding protein, at 127,977 to 128,990
GFF5262
GFF5263 - Ferric iron ABC transporter, permease protein, at 128,991 to 131,264
GFF5263
Position (kb)
127
128
129 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.986 kb on - strand, within GFF5261 at 127.171 kb on + strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.315 kb on + strand, within GFF5261 at 127.315 kb on + strand, within GFF5261 at 127.316 kb on - strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on - strand, within GFF5261 at 127.502 kb on - strand, within GFF5261 at 127.502 kb on - strand, within GFF5261 at 127.556 kb on - strand, within GFF5261 at 127.588 kb on + strand, within GFF5261 at 127.588 kb on + strand, within GFF5261 at 127.589 kb on - strand, within GFF5261 at 127.889 kb on + strand at 127.889 kb on + strand at 127.890 kb on - strand at 127.890 kb on - strand at 127.890 kb on - strand at 127.890 kb on - strand at 127.896 kb on - strand at 127.914 kb on + strand at 127.914 kb on + strand at 127.914 kb on + strand at 127.915 kb on - strand at 127.915 kb on - strand at 127.915 kb on - strand at 127.915 kb on - strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.924 kb on - strand at 127.924 kb on - strand at 128.097 kb on + strand, within GFF5262 at 128.097 kb on + strand, within GFF5262 at 128.098 kb on - strand, within GFF5262 at 128.098 kb on - strand, within GFF5262 at 128.105 kb on + strand, within GFF5262 at 128.106 kb on - strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.322 kb on + strand, within GFF5262 at 128.322 kb on + strand, within GFF5262 at 128.322 kb on + strand, within GFF5262 at 128.323 kb on - strand, within GFF5262 at 128.323 kb on - strand, within GFF5262 at 128.340 kb on + strand, within GFF5262 at 128.340 kb on + strand, within GFF5262 at 128.341 kb on - strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.436 kb on + strand, within GFF5262 at 128.437 kb on - strand, within GFF5262 at 128.437 kb on - strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.538 kb on + strand, within GFF5262 at 128.538 kb on + strand, within GFF5262 at 128.571 kb on + strand, within GFF5262 at 128.571 kb on + strand, within GFF5262 at 128.571 kb on + strand, within GFF5262 at 128.572 kb on - strand, within GFF5262 at 128.572 kb on - strand, within GFF5262 at 128.775 kb on + strand, within GFF5262 at 128.775 kb on + strand, within GFF5262 at 128.776 kb on - strand, within GFF5262 at 128.776 kb on - strand, within GFF5262 at 128.904 kb on + strand at 128.904 kb on + strand at 128.904 kb on + strand at 128.905 kb on - strand at 128.905 kb on - strand at 128.905 kb on - strand at 128.910 kb on + strand at 128.911 kb on - strand at 128.911 kb on - strand at 128.919 kb on + strand at 128.919 kb on + strand at 128.919 kb on + strand at 128.920 kb on - strand at 128.988 kb on + strand at 128.988 kb on + strand at 128.989 kb on - strand at 128.989 kb on - strand at 129.099 kb on + strand at 129.100 kb on - strand at 129.100 kb on - strand at 129.117 kb on + strand at 129.118 kb on - strand at 129.118 kb on - strand at 129.142 kb on - strand at 129.225 kb on + strand, within GFF5263 at 129.954 kb on + strand, within GFF5263 at 129.954 kb on + strand, within GFF5263 at 129.954 kb on + strand, within GFF5263 at 129.954 kb on + strand, within GFF5263 at 129.955 kb on - strand, within GFF5263 at 129.955 kb on - strand, within GFF5263 at 129.955 kb on - strand, within GFF5263
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 126,985 + GFF5261 0.42 -3.0 126,985 + GFF5261 0.42 -2.3 126,985 + GFF5261 0.42 -2.7 126,985 + GFF5261 0.42 -3.5 126,985 + GFF5261 0.42 -2.4 126,986 - GFF5261 0.42 -1.5 127,171 + GFF5261 0.55 +0.0 127,172 - GFF5261 0.55 -0.4 127,172 - GFF5261 0.55 -4.2 127,172 - GFF5261 0.55 -3.2 127,172 - GFF5261 0.55 -2.1 127,315 + GFF5261 0.65 -1.5 127,315 + GFF5261 0.65 -2.0 127,316 - GFF5261 0.65 -2.3 127,501 + GFF5261 0.77 -3.2 127,501 + GFF5261 0.77 -1.8 127,501 + GFF5261 0.77 +0.6 127,501 + GFF5261 0.77 -4.3 127,501 + GFF5261 0.77 -1.9 127,501 + GFF5261 0.77 -2.3 127,501 + GFF5261 0.77 -2.3 127,501 + GFF5261 0.77 -1.8 127,501 + GFF5261 0.77 -1.0 127,501 + GFF5261 0.77 -2.0 127,501 + GFF5261 0.77 -1.2 127,501 + GFF5261 0.77 +0.6 127,501 - GFF5261 0.77 -2.8 127,502 - GFF5261 0.78 -2.1 127,502 - GFF5261 0.78 +0.0 127,556 - GFF5261 0.81 -4.8 127,588 + GFF5261 0.83 -4.2 127,588 + GFF5261 0.83 -2.7 127,589 - GFF5261 0.83 -1.0 127,889 + +0.8 127,889 + +0.2 127,890 - +0.2 127,890 - +0.4 127,890 - +0.2 127,890 - +0.6 127,896 - +0.5 127,914 + -0.3 127,914 + -1.0 127,914 + -0.1 127,915 - +0.2 127,915 - -0.5 127,915 - +0.8 127,915 - +0.6 127,917 + -1.2 127,917 + -0.4 127,917 + +0.8 127,917 + +0.6 127,917 + +1.2 127,917 + -0.2 127,917 + +0.4 127,918 - -0.0 127,918 - +1.6 127,918 - -0.4 127,918 - +0.3 127,918 - +0.4 127,924 - +0.1 127,924 - -1.4 128,097 + GFF5262 0.12 -0.7 128,097 + GFF5262 0.12 +0.3 128,098 - GFF5262 0.12 +0.1 128,098 - GFF5262 0.12 -1.0 128,105 + GFF5262 0.13 +0.5 128,106 - GFF5262 0.13 +0.3 128,259 + GFF5262 0.28 +0.6 128,259 + GFF5262 0.28 +0.0 128,259 + GFF5262 0.28 +0.4 128,259 + GFF5262 0.28 -0.5 128,259 + GFF5262 0.28 -0.4 128,259 + GFF5262 0.28 -0.4 128,259 + GFF5262 0.28 +1.4 128,259 + GFF5262 0.28 +0.2 128,259 + GFF5262 0.28 +1.1 128,260 - GFF5262 0.28 +0.9 128,260 - GFF5262 0.28 +0.4 128,260 - GFF5262 0.28 -0.0 128,260 - GFF5262 0.28 -2.3 128,322 + GFF5262 0.34 -0.7 128,322 + GFF5262 0.34 +0.0 128,322 + GFF5262 0.34 +1.2 128,323 - GFF5262 0.34 -0.4 128,323 - GFF5262 0.34 -0.5 128,340 + GFF5262 0.36 +0.6 128,340 + GFF5262 0.36 +0.7 128,341 - GFF5262 0.36 -0.7 128,364 + GFF5262 0.38 -1.3 128,364 + GFF5262 0.38 +0.8 128,364 + GFF5262 0.38 +2.4 128,364 + GFF5262 0.38 +0.9 128,364 + GFF5262 0.38 -0.6 128,364 + GFF5262 0.38 +1.5 128,365 - GFF5262 0.38 +0.3 128,365 - GFF5262 0.38 +0.3 128,365 - GFF5262 0.38 +0.8 128,365 - GFF5262 0.38 +1.3 128,365 - GFF5262 0.38 +0.8 128,436 + GFF5262 0.45 +2.6 128,437 - GFF5262 0.45 -0.3 128,437 - GFF5262 0.45 -0.6 128,484 + GFF5262 0.50 +0.6 128,484 + GFF5262 0.50 -0.6 128,484 + GFF5262 0.50 +0.5 128,484 + GFF5262 0.50 +1.4 128,485 - GFF5262 0.50 +0.0 128,485 - GFF5262 0.50 +0.3 128,485 - GFF5262 0.50 +0.6 128,485 - GFF5262 0.50 -0.2 128,538 + GFF5262 0.55 +0.7 128,538 + GFF5262 0.55 +0.6 128,571 + GFF5262 0.59 +0.8 128,571 + GFF5262 0.59 +0.4 128,571 + GFF5262 0.59 +0.0 128,572 - GFF5262 0.59 -0.3 128,572 - GFF5262 0.59 +0.5 128,775 + GFF5262 0.79 +0.5 128,775 + GFF5262 0.79 +0.2 128,776 - GFF5262 0.79 +0.2 128,776 - GFF5262 0.79 -0.6 128,904 + +1.2 128,904 + -0.1 128,904 + -0.2 128,905 - +0.2 128,905 - -0.3 128,905 - -0.2 128,910 + -0.6 128,911 - -0.4 128,911 - +0.1 128,919 + +0.1 128,919 + +0.3 128,919 + +0.3 128,920 - +1.0 128,988 + +0.0 128,988 + -0.3 128,989 - +1.2 128,989 - +0.9 129,099 + +0.2 129,100 - +0.8 129,100 - +0.1 129,117 + +0.2 129,118 - +0.6 129,118 - +0.2 129,142 - +0.4 129,225 + GFF5263 0.10 -0.2 129,954 + GFF5263 0.42 +0.0 129,954 + GFF5263 0.42 +2.4 129,954 + GFF5263 0.42 +0.6 129,954 + GFF5263 0.42 +0.6 129,955 - GFF5263 0.42 +0.5 129,955 - GFF5263 0.42 +0.2 129,955 - GFF5263 0.42 -2.3
Or see this region's nucleotide sequence