Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5260 and GFF5261 are separated by 18 nucleotides GFF5261 and GFF5262 are separated by 144 nucleotides
GFF5260 - Uncharacterized protein ygiD, at 125,433 to 126,341
GFF5260
GFF5261 - Glucose-6-phosphate 1-dehydrogenase (EC 1.1.1.49), at 126,360 to 127,832
GFF5261
GFF5262 - Ferric iron ABC transporter, iron-binding protein, at 127,977 to 128,990
GFF5262
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 125.417 kb on - strand at 125.417 kb on - strand at 125.794 kb on - strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.911 kb on - strand, within GFF5260 at 125.959 kb on - strand, within GFF5260 at 125.982 kb on + strand, within GFF5260 at 125.983 kb on - strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.229 kb on - strand, within GFF5260 at 126.229 kb on - strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.352 kb on - strand at 126.361 kb on + strand at 126.361 kb on + strand at 126.361 kb on + strand at 126.362 kb on - strand at 126.362 kb on - strand at 126.362 kb on - strand at 126.362 kb on - strand at 126.571 kb on + strand, within GFF5261 at 126.571 kb on + strand, within GFF5261 at 126.847 kb on + strand, within GFF5261 at 126.847 kb on + strand, within GFF5261 at 126.848 kb on - strand, within GFF5261 at 126.848 kb on - strand, within GFF5261 at 126.848 kb on - strand, within GFF5261 at 126.848 kb on - strand, within GFF5261 at 126.967 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.985 kb on + strand, within GFF5261 at 126.986 kb on - strand, within GFF5261 at 127.171 kb on + strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.172 kb on - strand, within GFF5261 at 127.315 kb on + strand, within GFF5261 at 127.315 kb on + strand, within GFF5261 at 127.316 kb on - strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on + strand, within GFF5261 at 127.501 kb on - strand, within GFF5261 at 127.502 kb on - strand, within GFF5261 at 127.502 kb on - strand, within GFF5261 at 127.556 kb on - strand, within GFF5261 at 127.588 kb on + strand, within GFF5261 at 127.588 kb on + strand, within GFF5261 at 127.589 kb on - strand, within GFF5261 at 127.889 kb on + strand at 127.889 kb on + strand at 127.890 kb on - strand at 127.890 kb on - strand at 127.890 kb on - strand at 127.890 kb on - strand at 127.896 kb on - strand at 127.914 kb on + strand at 127.914 kb on + strand at 127.914 kb on + strand at 127.915 kb on - strand at 127.915 kb on - strand at 127.915 kb on - strand at 127.915 kb on - strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.917 kb on + strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.918 kb on - strand at 127.924 kb on - strand at 127.924 kb on - strand at 128.097 kb on + strand, within GFF5262 at 128.097 kb on + strand, within GFF5262 at 128.098 kb on - strand, within GFF5262 at 128.098 kb on - strand, within GFF5262 at 128.105 kb on + strand, within GFF5262 at 128.106 kb on - strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.259 kb on + strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.260 kb on - strand, within GFF5262 at 128.322 kb on + strand, within GFF5262 at 128.322 kb on + strand, within GFF5262 at 128.322 kb on + strand, within GFF5262 at 128.323 kb on - strand, within GFF5262 at 128.323 kb on - strand, within GFF5262 at 128.340 kb on + strand, within GFF5262 at 128.340 kb on + strand, within GFF5262 at 128.341 kb on - strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.364 kb on + strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.365 kb on - strand, within GFF5262 at 128.436 kb on + strand, within GFF5262 at 128.437 kb on - strand, within GFF5262 at 128.437 kb on - strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.484 kb on + strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.485 kb on - strand, within GFF5262 at 128.538 kb on + strand, within GFF5262 at 128.538 kb on + strand, within GFF5262 at 128.571 kb on + strand, within GFF5262 at 128.571 kb on + strand, within GFF5262 at 128.571 kb on + strand, within GFF5262 at 128.572 kb on - strand, within GFF5262 at 128.572 kb on - strand, within GFF5262 at 128.775 kb on + strand, within GFF5262 at 128.775 kb on + strand, within GFF5262 at 128.776 kb on - strand, within GFF5262 at 128.776 kb on - strand, within GFF5262
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 125,417 - +1.0 125,417 - -0.0 125,794 - GFF5260 0.40 +0.5 125,910 + GFF5260 0.52 -0.4 125,910 + GFF5260 0.52 +0.1 125,910 + GFF5260 0.52 -0.4 125,910 + GFF5260 0.52 +0.2 125,910 + GFF5260 0.52 -0.4 125,910 + GFF5260 0.52 -1.4 125,910 + GFF5260 0.52 +0.4 125,910 + GFF5260 0.52 -0.2 125,911 - GFF5260 0.53 -0.4 125,959 - GFF5260 0.58 -0.4 125,982 + GFF5260 0.60 -0.2 125,983 - GFF5260 0.61 -0.6 126,228 + GFF5260 0.87 -0.0 126,228 + GFF5260 0.87 -0.1 126,228 + GFF5260 0.87 +0.6 126,228 + GFF5260 0.87 -0.2 126,228 + GFF5260 0.87 -0.6 126,229 - GFF5260 0.88 +0.2 126,229 - GFF5260 0.88 -0.0 126,231 + GFF5260 0.88 +1.2 126,231 + GFF5260 0.88 +0.9 126,231 + GFF5260 0.88 -0.2 126,231 + GFF5260 0.88 +0.4 126,231 + GFF5260 0.88 -0.5 126,231 + GFF5260 0.88 +0.4 126,232 - GFF5260 0.88 +0.3 126,232 - GFF5260 0.88 +0.1 126,232 - GFF5260 0.88 -1.5 126,288 + +0.1 126,288 + -0.7 126,288 + +0.1 126,288 + -0.6 126,288 + +0.6 126,288 + -0.5 126,289 - +0.7 126,289 - -0.3 126,289 - +0.7 126,289 - -0.4 126,289 - -0.9 126,289 - +0.6 126,352 - +1.2 126,361 + -0.5 126,361 + -1.4 126,361 + -1.0 126,362 - +0.0 126,362 - -0.3 126,362 - -0.3 126,362 - -0.1 126,571 + GFF5261 0.14 -1.7 126,571 + GFF5261 0.14 -2.3 126,847 + GFF5261 0.33 -2.9 126,847 + GFF5261 0.33 -2.8 126,848 - GFF5261 0.33 -2.0 126,848 - GFF5261 0.33 -0.7 126,848 - GFF5261 0.33 -3.7 126,848 - GFF5261 0.33 -1.2 126,967 + GFF5261 0.41 -3.0 126,985 + GFF5261 0.42 -3.0 126,985 + GFF5261 0.42 -2.3 126,985 + GFF5261 0.42 -2.7 126,985 + GFF5261 0.42 -3.5 126,985 + GFF5261 0.42 -2.4 126,986 - GFF5261 0.42 -1.5 127,171 + GFF5261 0.55 +0.0 127,172 - GFF5261 0.55 -0.4 127,172 - GFF5261 0.55 -4.2 127,172 - GFF5261 0.55 -3.2 127,172 - GFF5261 0.55 -2.1 127,315 + GFF5261 0.65 -1.5 127,315 + GFF5261 0.65 -2.0 127,316 - GFF5261 0.65 -2.3 127,501 + GFF5261 0.77 -3.2 127,501 + GFF5261 0.77 -1.8 127,501 + GFF5261 0.77 +0.6 127,501 + GFF5261 0.77 -4.3 127,501 + GFF5261 0.77 -1.9 127,501 + GFF5261 0.77 -2.3 127,501 + GFF5261 0.77 -2.3 127,501 + GFF5261 0.77 -1.8 127,501 + GFF5261 0.77 -1.0 127,501 + GFF5261 0.77 -2.0 127,501 + GFF5261 0.77 -1.2 127,501 + GFF5261 0.77 +0.6 127,501 - GFF5261 0.77 -2.8 127,502 - GFF5261 0.78 -2.1 127,502 - GFF5261 0.78 +0.0 127,556 - GFF5261 0.81 -4.8 127,588 + GFF5261 0.83 -4.2 127,588 + GFF5261 0.83 -2.7 127,589 - GFF5261 0.83 -1.0 127,889 + +0.8 127,889 + +0.2 127,890 - +0.2 127,890 - +0.4 127,890 - +0.2 127,890 - +0.6 127,896 - +0.5 127,914 + -0.3 127,914 + -1.0 127,914 + -0.1 127,915 - +0.2 127,915 - -0.5 127,915 - +0.8 127,915 - +0.6 127,917 + -1.2 127,917 + -0.4 127,917 + +0.8 127,917 + +0.6 127,917 + +1.2 127,917 + -0.2 127,917 + +0.4 127,918 - -0.0 127,918 - +1.6 127,918 - -0.4 127,918 - +0.3 127,918 - +0.4 127,924 - +0.1 127,924 - -1.4 128,097 + GFF5262 0.12 -0.7 128,097 + GFF5262 0.12 +0.3 128,098 - GFF5262 0.12 +0.1 128,098 - GFF5262 0.12 -1.0 128,105 + GFF5262 0.13 +0.5 128,106 - GFF5262 0.13 +0.3 128,259 + GFF5262 0.28 +0.6 128,259 + GFF5262 0.28 +0.0 128,259 + GFF5262 0.28 +0.4 128,259 + GFF5262 0.28 -0.5 128,259 + GFF5262 0.28 -0.4 128,259 + GFF5262 0.28 -0.4 128,259 + GFF5262 0.28 +1.4 128,259 + GFF5262 0.28 +0.2 128,259 + GFF5262 0.28 +1.1 128,260 - GFF5262 0.28 +0.9 128,260 - GFF5262 0.28 +0.4 128,260 - GFF5262 0.28 -0.0 128,260 - GFF5262 0.28 -2.3 128,322 + GFF5262 0.34 -0.7 128,322 + GFF5262 0.34 +0.0 128,322 + GFF5262 0.34 +1.2 128,323 - GFF5262 0.34 -0.4 128,323 - GFF5262 0.34 -0.5 128,340 + GFF5262 0.36 +0.6 128,340 + GFF5262 0.36 +0.7 128,341 - GFF5262 0.36 -0.7 128,364 + GFF5262 0.38 -1.3 128,364 + GFF5262 0.38 +0.8 128,364 + GFF5262 0.38 +2.4 128,364 + GFF5262 0.38 +0.9 128,364 + GFF5262 0.38 -0.6 128,364 + GFF5262 0.38 +1.5 128,365 - GFF5262 0.38 +0.3 128,365 - GFF5262 0.38 +0.3 128,365 - GFF5262 0.38 +0.8 128,365 - GFF5262 0.38 +1.3 128,365 - GFF5262 0.38 +0.8 128,436 + GFF5262 0.45 +2.6 128,437 - GFF5262 0.45 -0.3 128,437 - GFF5262 0.45 -0.6 128,484 + GFF5262 0.50 +0.6 128,484 + GFF5262 0.50 -0.6 128,484 + GFF5262 0.50 +0.5 128,484 + GFF5262 0.50 +1.4 128,485 - GFF5262 0.50 +0.0 128,485 - GFF5262 0.50 +0.3 128,485 - GFF5262 0.50 +0.6 128,485 - GFF5262 0.50 -0.2 128,538 + GFF5262 0.55 +0.7 128,538 + GFF5262 0.55 +0.6 128,571 + GFF5262 0.59 +0.8 128,571 + GFF5262 0.59 +0.4 128,571 + GFF5262 0.59 +0.0 128,572 - GFF5262 0.59 -0.3 128,572 - GFF5262 0.59 +0.5 128,775 + GFF5262 0.79 +0.5 128,775 + GFF5262 0.79 +0.2 128,776 - GFF5262 0.79 +0.2 128,776 - GFF5262 0.79 -0.6
Or see this region's nucleotide sequence