Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5257 and GFF5258 are separated by 94 nucleotides GFF5258 and GFF5259 overlap by 1 nucleotides GFF5259 and GFF5260 are separated by 73 nucleotides
GFF5257 - ABC transporter, ATP-binding protein (cluster 5, nickel/peptides/opines), at 122,543 to 123,580
GFF5257
GFF5258 - hypothetical protein, at 123,675 to 123,854
GFF5258
GFF5259 - Na+/solute symporter, at 123,854 to 125,359
GFF5259
GFF5260 - Uncharacterized protein ygiD, at 125,433 to 126,341
GFF5260
Position (kb) 123
124
125
126 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 122.909 kb on + strand, within GFF5257 at 122.910 kb on - strand, within GFF5257 at 123.059 kb on + strand, within GFF5257 at 123.059 kb on + strand, within GFF5257 at 123.059 kb on + strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.060 kb on - strand, within GFF5257 at 123.230 kb on + strand, within GFF5257 at 123.230 kb on + strand, within GFF5257 at 123.230 kb on + strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.231 kb on - strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.293 kb on + strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.294 kb on - strand, within GFF5257 at 123.356 kb on + strand, within GFF5257 at 123.357 kb on - strand, within GFF5257 at 123.357 kb on - strand, within GFF5257 at 123.578 kb on + strand at 123.592 kb on - strand at 123.627 kb on + strand at 123.627 kb on + strand at 123.627 kb on + strand at 123.627 kb on + strand at 123.628 kb on - strand at 123.628 kb on - strand at 123.629 kb on + strand at 123.629 kb on + strand at 123.629 kb on + strand at 123.629 kb on + strand at 123.630 kb on - strand at 123.630 kb on - strand at 123.630 kb on - strand at 123.726 kb on + strand, within GFF5258 at 123.727 kb on - strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.768 kb on + strand, within GFF5258 at 123.769 kb on - strand, within GFF5258 at 123.769 kb on - strand, within GFF5258 at 123.769 kb on - strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.774 kb on + strand, within GFF5258 at 123.775 kb on - strand, within GFF5258 at 123.775 kb on - strand, within GFF5258 at 123.775 kb on - strand, within GFF5258 at 123.823 kb on - strand, within GFF5258 at 123.834 kb on + strand, within GFF5258 at 123.835 kb on - strand, within GFF5258 at 124.049 kb on + strand, within GFF5259 at 124.050 kb on - strand, within GFF5259 at 124.050 kb on - strand, within GFF5259 at 124.050 kb on - strand, within GFF5259 at 124.058 kb on + strand, within GFF5259 at 124.058 kb on + strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.059 kb on - strand, within GFF5259 at 124.088 kb on + strand, within GFF5259 at 124.089 kb on - strand, within GFF5259 at 124.103 kb on + strand, within GFF5259 at 124.103 kb on + strand, within GFF5259 at 124.103 kb on + strand, within GFF5259 at 124.124 kb on + strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.125 kb on - strand, within GFF5259 at 124.220 kb on + strand, within GFF5259 at 124.221 kb on - strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.289 kb on + strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.290 kb on - strand, within GFF5259 at 124.388 kb on + strand, within GFF5259 at 124.388 kb on + strand, within GFF5259 at 124.388 kb on + strand, within GFF5259 at 124.389 kb on - strand, within GFF5259 at 124.389 kb on - strand, within GFF5259 at 124.394 kb on + strand, within GFF5259 at 124.394 kb on + strand, within GFF5259 at 124.394 kb on + strand, within GFF5259 at 124.395 kb on - strand, within GFF5259 at 124.395 kb on - strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.451 kb on + strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.452 kb on - strand, within GFF5259 at 124.583 kb on + strand, within GFF5259 at 124.583 kb on + strand, within GFF5259 at 124.583 kb on + strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.584 kb on - strand, within GFF5259 at 124.592 kb on + strand, within GFF5259 at 124.592 kb on + strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.593 kb on - strand, within GFF5259 at 124.634 kb on + strand, within GFF5259 at 124.634 kb on + strand, within GFF5259 at 124.634 kb on + strand, within GFF5259 at 124.635 kb on - strand, within GFF5259 at 124.635 kb on - strand, within GFF5259 at 124.635 kb on - strand, within GFF5259 at 124.712 kb on + strand, within GFF5259 at 124.712 kb on + strand, within GFF5259 at 124.712 kb on + strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.713 kb on - strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.748 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.937 kb on + strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 124.938 kb on - strand, within GFF5259 at 125.179 kb on + strand, within GFF5259 at 125.179 kb on + strand, within GFF5259 at 125.252 kb on + strand at 125.358 kb on - strand at 125.417 kb on - strand at 125.417 kb on - strand at 125.794 kb on - strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.910 kb on + strand, within GFF5260 at 125.911 kb on - strand, within GFF5260 at 125.959 kb on - strand, within GFF5260 at 125.982 kb on + strand, within GFF5260 at 125.983 kb on - strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.228 kb on + strand, within GFF5260 at 126.229 kb on - strand, within GFF5260 at 126.229 kb on - strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.231 kb on + strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.232 kb on - strand, within GFF5260 at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.288 kb on + strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.289 kb on - strand at 126.352 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 122,909 + GFF5257 0.35 -0.6 122,910 - GFF5257 0.35 -0.3 123,059 + GFF5257 0.50 -0.3 123,059 + GFF5257 0.50 +0.0 123,059 + GFF5257 0.50 -1.8 123,060 - GFF5257 0.50 -0.4 123,060 - GFF5257 0.50 +0.5 123,060 - GFF5257 0.50 -1.2 123,060 - GFF5257 0.50 -0.4 123,230 + GFF5257 0.66 -0.1 123,230 + GFF5257 0.66 +0.4 123,230 + GFF5257 0.66 +0.0 123,231 - GFF5257 0.66 -0.4 123,231 - GFF5257 0.66 -0.6 123,231 - GFF5257 0.66 +0.2 123,231 - GFF5257 0.66 -0.6 123,231 - GFF5257 0.66 +0.9 123,231 - GFF5257 0.66 +0.2 123,293 + GFF5257 0.72 -0.4 123,293 + GFF5257 0.72 -0.6 123,293 + GFF5257 0.72 +0.0 123,293 + GFF5257 0.72 -1.1 123,293 + GFF5257 0.72 -0.3 123,293 + GFF5257 0.72 +0.6 123,294 - GFF5257 0.72 +1.6 123,294 - GFF5257 0.72 +0.4 123,294 - GFF5257 0.72 -0.3 123,294 - GFF5257 0.72 -2.2 123,294 - GFF5257 0.72 -1.7 123,294 - GFF5257 0.72 -0.1 123,294 - GFF5257 0.72 +2.6 123,356 + GFF5257 0.78 +1.1 123,357 - GFF5257 0.78 +1.4 123,357 - GFF5257 0.78 -0.7 123,578 + -0.2 123,592 - -0.6 123,627 + -0.9 123,627 + +0.9 123,627 + -0.1 123,627 + +0.0 123,628 - +0.1 123,628 - +1.6 123,629 + -0.8 123,629 + -1.0 123,629 + -0.4 123,629 + +0.4 123,630 - +0.1 123,630 - -0.3 123,630 - +0.0 123,726 + GFF5258 0.28 -0.0 123,727 - GFF5258 0.29 +0.1 123,768 + GFF5258 0.52 -0.3 123,768 + GFF5258 0.52 -0.0 123,768 + GFF5258 0.52 +1.0 123,768 + GFF5258 0.52 +0.1 123,768 + GFF5258 0.52 -0.0 123,769 - GFF5258 0.52 -0.7 123,769 - GFF5258 0.52 +0.0 123,769 - GFF5258 0.52 -2.3 123,774 + GFF5258 0.55 +0.0 123,774 + GFF5258 0.55 +0.2 123,774 + GFF5258 0.55 +0.6 123,774 + GFF5258 0.55 -1.7 123,775 - GFF5258 0.56 +0.1 123,775 - GFF5258 0.56 -0.1 123,775 - GFF5258 0.56 -1.7 123,823 - GFF5258 0.82 -0.0 123,834 + GFF5258 0.88 -0.8 123,835 - GFF5258 0.89 -1.4 124,049 + GFF5259 0.13 +0.2 124,050 - GFF5259 0.13 -0.2 124,050 - GFF5259 0.13 +0.4 124,050 - GFF5259 0.13 +0.5 124,058 + GFF5259 0.14 -0.4 124,058 + GFF5259 0.14 -0.3 124,059 - GFF5259 0.14 -0.2 124,059 - GFF5259 0.14 -0.4 124,059 - GFF5259 0.14 +0.4 124,059 - GFF5259 0.14 -1.0 124,088 + GFF5259 0.16 -0.2 124,089 - GFF5259 0.16 -0.1 124,103 + GFF5259 0.17 -0.4 124,103 + GFF5259 0.17 -2.0 124,103 + GFF5259 0.17 -0.2 124,124 + GFF5259 0.18 -0.5 124,125 - GFF5259 0.18 +0.6 124,125 - GFF5259 0.18 -1.0 124,125 - GFF5259 0.18 -1.3 124,125 - GFF5259 0.18 +0.4 124,125 - GFF5259 0.18 +0.5 124,125 - GFF5259 0.18 -0.1 124,220 + GFF5259 0.24 +0.9 124,221 - GFF5259 0.24 +0.7 124,289 + GFF5259 0.29 +0.5 124,289 + GFF5259 0.29 -0.1 124,289 + GFF5259 0.29 +0.0 124,289 + GFF5259 0.29 +0.7 124,289 + GFF5259 0.29 -0.2 124,289 + GFF5259 0.29 +0.4 124,290 - GFF5259 0.29 +0.5 124,290 - GFF5259 0.29 -0.1 124,290 - GFF5259 0.29 -0.5 124,290 - GFF5259 0.29 +0.8 124,290 - GFF5259 0.29 -0.5 124,290 - GFF5259 0.29 +1.8 124,290 - GFF5259 0.29 -0.3 124,290 - GFF5259 0.29 -0.0 124,388 + GFF5259 0.35 +0.0 124,388 + GFF5259 0.35 -0.3 124,388 + GFF5259 0.35 +0.4 124,389 - GFF5259 0.36 -0.4 124,389 - GFF5259 0.36 +0.1 124,394 + GFF5259 0.36 -0.1 124,394 + GFF5259 0.36 -0.3 124,394 + GFF5259 0.36 +0.9 124,395 - GFF5259 0.36 -0.3 124,395 - GFF5259 0.36 +0.6 124,451 + GFF5259 0.40 +0.2 124,451 + GFF5259 0.40 -0.7 124,451 + GFF5259 0.40 +0.6 124,451 + GFF5259 0.40 -0.8 124,451 + GFF5259 0.40 -0.2 124,452 - GFF5259 0.40 +1.0 124,452 - GFF5259 0.40 -0.4 124,452 - GFF5259 0.40 +0.0 124,452 - GFF5259 0.40 +0.1 124,452 - GFF5259 0.40 -0.3 124,452 - GFF5259 0.40 +0.0 124,452 - GFF5259 0.40 +0.0 124,452 - GFF5259 0.40 +0.0 124,452 - GFF5259 0.40 +0.1 124,452 - GFF5259 0.40 -0.3 124,452 - GFF5259 0.40 -3.0 124,452 - GFF5259 0.40 +0.1 124,452 - GFF5259 0.40 -0.9 124,583 + GFF5259 0.48 +0.1 124,583 + GFF5259 0.48 +1.3 124,583 + GFF5259 0.48 +1.0 124,584 - GFF5259 0.48 +1.8 124,584 - GFF5259 0.48 -0.4 124,584 - GFF5259 0.48 +0.0 124,584 - GFF5259 0.48 -0.9 124,592 + GFF5259 0.49 -0.3 124,592 + GFF5259 0.49 -1.0 124,593 - GFF5259 0.49 -0.5 124,593 - GFF5259 0.49 -2.3 124,593 - GFF5259 0.49 -1.1 124,593 - GFF5259 0.49 +1.6 124,634 + GFF5259 0.52 +0.2 124,634 + GFF5259 0.52 -0.7 124,634 + GFF5259 0.52 +1.0 124,635 - GFF5259 0.52 +0.6 124,635 - GFF5259 0.52 +0.0 124,635 - GFF5259 0.52 +0.6 124,712 + GFF5259 0.57 +0.7 124,712 + GFF5259 0.57 +0.8 124,712 + GFF5259 0.57 -0.4 124,713 - GFF5259 0.57 +0.3 124,713 - GFF5259 0.57 +0.2 124,713 - GFF5259 0.57 -0.1 124,713 - GFF5259 0.57 +0.7 124,713 - GFF5259 0.57 +0.2 124,748 + GFF5259 0.59 -0.5 124,748 + GFF5259 0.59 +0.0 124,748 + GFF5259 0.59 -0.2 124,748 + GFF5259 0.59 -1.5 124,748 + GFF5259 0.59 +0.3 124,748 + GFF5259 0.59 +0.7 124,748 + GFF5259 0.59 +0.9 124,748 + GFF5259 0.59 +0.0 124,937 + GFF5259 0.72 -0.6 124,937 + GFF5259 0.72 +1.9 124,937 + GFF5259 0.72 +0.2 124,937 + GFF5259 0.72 -1.1 124,937 + GFF5259 0.72 +0.6 124,937 + GFF5259 0.72 +0.5 124,937 + GFF5259 0.72 -1.0 124,937 + GFF5259 0.72 +1.2 124,938 - GFF5259 0.72 -2.1 124,938 - GFF5259 0.72 +0.4 124,938 - GFF5259 0.72 +0.3 124,938 - GFF5259 0.72 +0.4 124,938 - GFF5259 0.72 +1.0 124,938 - GFF5259 0.72 +0.4 124,938 - GFF5259 0.72 +1.0 124,938 - GFF5259 0.72 +0.5 124,938 - GFF5259 0.72 -0.3 124,938 - GFF5259 0.72 -0.4 124,938 - GFF5259 0.72 +0.5 124,938 - GFF5259 0.72 -0.4 124,938 - GFF5259 0.72 -1.0 124,938 - GFF5259 0.72 +0.6 124,938 - GFF5259 0.72 +0.5 125,179 + GFF5259 0.88 +0.6 125,179 + GFF5259 0.88 +0.1 125,252 + +0.5 125,358 - +0.0 125,417 - +1.0 125,417 - -0.0 125,794 - GFF5260 0.40 +0.5 125,910 + GFF5260 0.52 -0.4 125,910 + GFF5260 0.52 +0.1 125,910 + GFF5260 0.52 -0.4 125,910 + GFF5260 0.52 +0.2 125,910 + GFF5260 0.52 -0.4 125,910 + GFF5260 0.52 -1.4 125,910 + GFF5260 0.52 +0.4 125,910 + GFF5260 0.52 -0.2 125,911 - GFF5260 0.53 -0.4 125,959 - GFF5260 0.58 -0.4 125,982 + GFF5260 0.60 -0.2 125,983 - GFF5260 0.61 -0.6 126,228 + GFF5260 0.87 -0.0 126,228 + GFF5260 0.87 -0.1 126,228 + GFF5260 0.87 +0.6 126,228 + GFF5260 0.87 -0.2 126,228 + GFF5260 0.87 -0.6 126,229 - GFF5260 0.88 +0.2 126,229 - GFF5260 0.88 -0.0 126,231 + GFF5260 0.88 +1.2 126,231 + GFF5260 0.88 +0.9 126,231 + GFF5260 0.88 -0.2 126,231 + GFF5260 0.88 +0.4 126,231 + GFF5260 0.88 -0.5 126,231 + GFF5260 0.88 +0.4 126,232 - GFF5260 0.88 +0.3 126,232 - GFF5260 0.88 +0.1 126,232 - GFF5260 0.88 -1.5 126,288 + +0.1 126,288 + -0.7 126,288 + +0.1 126,288 + -0.6 126,288 + +0.6 126,288 + -0.5 126,289 - +0.7 126,289 - -0.3 126,289 - +0.7 126,289 - -0.4 126,289 - -0.9 126,289 - +0.6 126,352 - +1.2
Or see this region's nucleotide sequence