Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5244 and GFF5245 are separated by 136 nucleotides GFF5245 and GFF5246 are separated by 67 nucleotides GFF5246 and GFF5247 are separated by 74 nucleotides
GFF5244 - General secretion pathway protein G, at 108,079 to 108,570
GFF5244
GFF5245 - FIG00771430: hypothetical protein, at 108,707 to 109,186
GFF5245
GFF5246 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 109,254 to 110,834
GFF5246
GFF5247 - hypothetical protein, at 110,909 to 112,039
GFF5247
Position (kb)
109
110
111 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3 at 108.326 kb on + strand, within GFF5244 at 108.490 kb on - strand, within GFF5244 at 108.513 kb on + strand, within GFF5244 at 108.514 kb on - strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.519 kb on + strand, within GFF5244 at 108.520 kb on - strand, within GFF5244 at 108.520 kb on - strand, within GFF5244 at 108.520 kb on - strand, within GFF5244 at 108.520 kb on - strand, within GFF5244 at 108.520 kb on - strand, within GFF5244 at 108.520 kb on - strand, within GFF5244 at 108.581 kb on + strand at 108.581 kb on + strand at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.923 kb on + strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 108.924 kb on - strand, within GFF5245 at 109.277 kb on - strand at 109.303 kb on + strand at 109.304 kb on - strand at 109.304 kb on - strand at 109.309 kb on + strand at 109.693 kb on + strand, within GFF5246 at 109.693 kb on + strand, within GFF5246 at 109.694 kb on - strand, within GFF5246 at 109.749 kb on + strand, within GFF5246 at 109.907 kb on - strand, within GFF5246 at 109.907 kb on - strand, within GFF5246 at 109.940 kb on - strand, within GFF5246 at 109.940 kb on - strand, within GFF5246 at 110.050 kb on + strand, within GFF5246 at 110.051 kb on - strand, within GFF5246 at 110.051 kb on - strand, within GFF5246 at 110.051 kb on - strand, within GFF5246 at 110.205 kb on + strand, within GFF5246 at 110.205 kb on + strand, within GFF5246 at 110.206 kb on - strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.239 kb on + strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.240 kb on - strand, within GFF5246 at 110.247 kb on + strand, within GFF5246 at 110.422 kb on + strand, within GFF5246 at 110.422 kb on + strand, within GFF5246 at 110.423 kb on - strand, within GFF5246 at 110.423 kb on - strand, within GFF5246 at 110.423 kb on - strand, within GFF5246 at 110.423 kb on - strand, within GFF5246 at 110.423 kb on - strand, within GFF5246 at 110.423 kb on - strand, within GFF5246 at 110.434 kb on + strand, within GFF5246 at 110.434 kb on + strand, within GFF5246 at 110.434 kb on + strand, within GFF5246 at 110.434 kb on + strand, within GFF5246 at 110.435 kb on - strand, within GFF5246 at 110.435 kb on - strand, within GFF5246 at 110.435 kb on - strand, within GFF5246 at 110.636 kb on + strand, within GFF5246 at 110.636 kb on + strand, within GFF5246 at 110.636 kb on + strand, within GFF5246 at 110.637 kb on - strand, within GFF5246 at 110.637 kb on - strand, within GFF5246 at 110.637 kb on - strand, within GFF5246 at 110.638 kb on + strand, within GFF5246 at 110.638 kb on + strand, within GFF5246 at 110.638 kb on + strand, within GFF5246 at 110.639 kb on - strand, within GFF5246 at 110.639 kb on - strand, within GFF5246 at 110.639 kb on - strand, within GFF5246 at 110.639 kb on - strand, within GFF5246 at 110.639 kb on - strand, within GFF5246 at 110.761 kb on + strand at 110.761 kb on + strand at 110.762 kb on - strand at 110.762 kb on - strand at 110.779 kb on - strand at 110.791 kb on + strand at 110.792 kb on - strand at 110.817 kb on - strand at 110.863 kb on + strand at 110.863 kb on + strand at 110.863 kb on + strand at 110.863 kb on + strand at 110.864 kb on - strand at 110.876 kb on + strand at 110.877 kb on - strand at 110.877 kb on - strand at 110.877 kb on - strand at 110.877 kb on - strand at 110.903 kb on + strand at 110.904 kb on - strand at 110.904 kb on - strand at 110.904 kb on - strand at 110.904 kb on - strand at 110.982 kb on - strand at 111.013 kb on + strand at 111.013 kb on + strand at 111.014 kb on - strand at 111.014 kb on - strand at 111.034 kb on - strand, within GFF5247 at 111.241 kb on + strand, within GFF5247 at 111.242 kb on - strand, within GFF5247 at 111.242 kb on - strand, within GFF5247 at 111.242 kb on - strand, within GFF5247 at 111.263 kb on + strand, within GFF5247 at 111.263 kb on + strand, within GFF5247 at 111.263 kb on + strand, within GFF5247 at 111.386 kb on + strand, within GFF5247 at 111.387 kb on - strand, within GFF5247 at 111.519 kb on + strand, within GFF5247 at 111.519 kb on + strand, within GFF5247 at 111.520 kb on - strand, within GFF5247 at 111.533 kb on - strand, within GFF5247 at 111.608 kb on + strand, within GFF5247 at 111.608 kb on + strand, within GFF5247 at 111.608 kb on + strand, within GFF5247 at 111.608 kb on + strand, within GFF5247 at 111.609 kb on - strand, within GFF5247 at 111.609 kb on - strand, within GFF5247 at 111.609 kb on - strand, within GFF5247 at 111.609 kb on - strand, within GFF5247 at 111.683 kb on + strand, within GFF5247 at 111.684 kb on - strand, within GFF5247 at 111.684 kb on - strand, within GFF5247 at 111.684 kb on - strand, within GFF5247 at 111.684 kb on - strand, within GFF5247 at 111.684 kb on - strand, within GFF5247 at 111.719 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.737 kb on + strand, within GFF5247 at 111.738 kb on - strand, within GFF5247 at 111.738 kb on - strand, within GFF5247 at 111.738 kb on - strand, within GFF5247 at 111.738 kb on - strand, within GFF5247 at 111.738 kb on - strand, within GFF5247
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 108,326 + GFF5244 0.50 -0.4 108,490 - GFF5244 0.84 +0.9 108,513 + GFF5244 0.88 -0.1 108,514 - GFF5244 0.88 -1.4 108,519 + GFF5244 0.89 -0.7 108,519 + GFF5244 0.89 +0.4 108,519 + GFF5244 0.89 -1.1 108,519 + GFF5244 0.89 -2.2 108,519 + GFF5244 0.89 -0.3 108,519 + GFF5244 0.89 +0.3 108,519 + GFF5244 0.89 +0.9 108,520 - GFF5244 0.90 +0.7 108,520 - GFF5244 0.90 +0.0 108,520 - GFF5244 0.90 -0.6 108,520 - GFF5244 0.90 -0.3 108,520 - GFF5244 0.90 -1.1 108,520 - GFF5244 0.90 -0.3 108,581 + -0.6 108,581 + -0.9 108,923 + GFF5245 0.45 -0.3 108,923 + GFF5245 0.45 -1.5 108,923 + GFF5245 0.45 -0.4 108,923 + GFF5245 0.45 -0.7 108,923 + GFF5245 0.45 +0.2 108,923 + GFF5245 0.45 +0.0 108,923 + GFF5245 0.45 +0.0 108,923 + GFF5245 0.45 -0.6 108,923 + GFF5245 0.45 -1.0 108,923 + GFF5245 0.45 -0.7 108,923 + GFF5245 0.45 -0.0 108,923 + GFF5245 0.45 -0.0 108,923 + GFF5245 0.45 -0.7 108,923 + GFF5245 0.45 -1.3 108,923 + GFF5245 0.45 -1.0 108,923 + GFF5245 0.45 +0.0 108,923 + GFF5245 0.45 -0.4 108,923 + GFF5245 0.45 -0.3 108,923 + GFF5245 0.45 -0.5 108,923 + GFF5245 0.45 -0.2 108,923 + GFF5245 0.45 +0.0 108,924 - GFF5245 0.45 -0.5 108,924 - GFF5245 0.45 -0.3 108,924 - GFF5245 0.45 +1.6 108,924 - GFF5245 0.45 -0.1 108,924 - GFF5245 0.45 -1.5 108,924 - GFF5245 0.45 -0.7 108,924 - GFF5245 0.45 +0.7 108,924 - GFF5245 0.45 -0.3 108,924 - GFF5245 0.45 -0.2 108,924 - GFF5245 0.45 -0.3 108,924 - GFF5245 0.45 +0.3 108,924 - GFF5245 0.45 +1.2 108,924 - GFF5245 0.45 +0.1 108,924 - GFF5245 0.45 -0.4 108,924 - GFF5245 0.45 -0.4 108,924 - GFF5245 0.45 -0.1 108,924 - GFF5245 0.45 +0.4 108,924 - GFF5245 0.45 +0.0 109,277 - -1.9 109,303 + -1.4 109,304 - -1.2 109,304 - +1.2 109,309 + -1.4 109,693 + GFF5246 0.28 -1.1 109,693 + GFF5246 0.28 -0.4 109,694 - GFF5246 0.28 -2.0 109,749 + GFF5246 0.31 -1.3 109,907 - GFF5246 0.41 +1.0 109,907 - GFF5246 0.41 -0.2 109,940 - GFF5246 0.43 -1.8 109,940 - GFF5246 0.43 -1.5 110,050 + GFF5246 0.50 -3.6 110,051 - GFF5246 0.50 -1.2 110,051 - GFF5246 0.50 -2.3 110,051 - GFF5246 0.50 -2.3 110,205 + GFF5246 0.60 -0.4 110,205 + GFF5246 0.60 -1.4 110,206 - GFF5246 0.60 -1.2 110,239 + GFF5246 0.62 -5.0 110,239 + GFF5246 0.62 -2.0 110,239 + GFF5246 0.62 -1.4 110,239 + GFF5246 0.62 -1.8 110,239 + GFF5246 0.62 -2.1 110,239 + GFF5246 0.62 -3.0 110,239 + GFF5246 0.62 -0.8 110,240 - GFF5246 0.62 -3.3 110,240 - GFF5246 0.62 -2.1 110,240 - GFF5246 0.62 -0.7 110,240 - GFF5246 0.62 -2.8 110,240 - GFF5246 0.62 -2.0 110,240 - GFF5246 0.62 -1.0 110,240 - GFF5246 0.62 +1.6 110,247 + GFF5246 0.63 -1.2 110,422 + GFF5246 0.74 -1.2 110,422 + GFF5246 0.74 -1.2 110,423 - GFF5246 0.74 -1.2 110,423 - GFF5246 0.74 -3.3 110,423 - GFF5246 0.74 -0.6 110,423 - GFF5246 0.74 -1.7 110,423 - GFF5246 0.74 -2.3 110,423 - GFF5246 0.74 -0.4 110,434 + GFF5246 0.75 -1.5 110,434 + GFF5246 0.75 -1.1 110,434 + GFF5246 0.75 -0.7 110,434 + GFF5246 0.75 -2.9 110,435 - GFF5246 0.75 -0.8 110,435 - GFF5246 0.75 -2.3 110,435 - GFF5246 0.75 -1.4 110,636 + GFF5246 0.87 -3.8 110,636 + GFF5246 0.87 -1.5 110,636 + GFF5246 0.87 -2.2 110,637 - GFF5246 0.87 -1.5 110,637 - GFF5246 0.87 +1.2 110,637 - GFF5246 0.87 -3.4 110,638 + GFF5246 0.88 -1.5 110,638 + GFF5246 0.88 -1.1 110,638 + GFF5246 0.88 -1.7 110,639 - GFF5246 0.88 -0.5 110,639 - GFF5246 0.88 -3.5 110,639 - GFF5246 0.88 -0.3 110,639 - GFF5246 0.88 -0.4 110,639 - GFF5246 0.88 -0.8 110,761 + -1.0 110,761 + -1.2 110,762 - -0.4 110,762 - -3.6 110,779 - -0.4 110,791 + -2.0 110,792 - -1.7 110,817 - +1.1 110,863 + +2.9 110,863 + -0.2 110,863 + +0.8 110,863 + -2.5 110,864 - -0.0 110,876 + +0.6 110,877 - -1.6 110,877 - -0.5 110,877 - -0.2 110,877 - +0.2 110,903 + -0.3 110,904 - +1.4 110,904 - -1.2 110,904 - -0.5 110,904 - -0.2 110,982 - +0.0 111,013 + -0.2 111,013 + -1.5 111,014 - -0.7 111,014 - +0.2 111,034 - GFF5247 0.11 -1.0 111,241 + GFF5247 0.29 +0.5 111,242 - GFF5247 0.29 -0.8 111,242 - GFF5247 0.29 -0.7 111,242 - GFF5247 0.29 -0.6 111,263 + GFF5247 0.31 +2.2 111,263 + GFF5247 0.31 +0.0 111,263 + GFF5247 0.31 -0.8 111,386 + GFF5247 0.42 +0.2 111,387 - GFF5247 0.42 -1.3 111,519 + GFF5247 0.54 -0.6 111,519 + GFF5247 0.54 +1.0 111,520 - GFF5247 0.54 -0.4 111,533 - GFF5247 0.55 -0.2 111,608 + GFF5247 0.62 -0.1 111,608 + GFF5247 0.62 +1.0 111,608 + GFF5247 0.62 +0.3 111,608 + GFF5247 0.62 -0.7 111,609 - GFF5247 0.62 -0.0 111,609 - GFF5247 0.62 -0.3 111,609 - GFF5247 0.62 -0.7 111,609 - GFF5247 0.62 -0.5 111,683 + GFF5247 0.68 -0.4 111,684 - GFF5247 0.69 -0.3 111,684 - GFF5247 0.69 +0.4 111,684 - GFF5247 0.69 -0.2 111,684 - GFF5247 0.69 -0.8 111,684 - GFF5247 0.69 +0.2 111,719 + GFF5247 0.72 -0.6 111,737 + GFF5247 0.73 -0.0 111,737 + GFF5247 0.73 +1.0 111,737 + GFF5247 0.73 +0.5 111,737 + GFF5247 0.73 +0.6 111,737 + GFF5247 0.73 +0.1 111,737 + GFF5247 0.73 +0.3 111,737 + GFF5247 0.73 -1.4 111,737 + GFF5247 0.73 -0.6 111,737 + GFF5247 0.73 -1.7 111,737 + GFF5247 0.73 -0.5 111,737 + GFF5247 0.73 -0.2 111,737 + GFF5247 0.73 -0.5 111,737 + GFF5247 0.73 -1.3 111,738 - GFF5247 0.73 -0.6 111,738 - GFF5247 0.73 -0.2 111,738 - GFF5247 0.73 -0.6 111,738 - GFF5247 0.73 -0.0 111,738 - GFF5247 0.73 -1.0
Or see this region's nucleotide sequence