Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5178 and GFF5179 overlap by 4 nucleotides GFF5179 and GFF5180 overlap by 4 nucleotides GFF5180 and GFF5181 are separated by 105 nucleotides GFF5181 and GFF5182 are separated by 732 nucleotides
GFF5178 - Transcriptional regulator, Xre-family with cupin domain, at 32,178 to 32,774
GFF5178
GFF5179 - Acetyltransferase, GNAT family, at 32,771 to 33,334
GFF5179
GFF5180 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 33,331 to 34,464
GFF5180
GFF5181 - hypothetical protein, at 34,570 to 34,683
GFF5181
GFF5182 - no description, at 35,416 to 36,468
GFF5182
Position (kb)
33
34
35 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 32.469 kb on + strand, within GFF5178 at 32.469 kb on + strand, within GFF5178 at 32.469 kb on + strand, within GFF5178 at 32.469 kb on + strand, within GFF5178 at 32.469 kb on + strand, within GFF5178 at 32.469 kb on + strand, within GFF5178 at 32.470 kb on - strand, within GFF5178 at 32.470 kb on - strand, within GFF5178 at 32.470 kb on - strand, within GFF5178 at 32.470 kb on - strand, within GFF5178 at 32.470 kb on - strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.471 kb on + strand, within GFF5178 at 32.472 kb on - strand, within GFF5178 at 32.472 kb on - strand, within GFF5178 at 32.472 kb on - strand, within GFF5178 at 32.556 kb on + strand, within GFF5178 at 32.556 kb on + strand, within GFF5178 at 32.556 kb on + strand, within GFF5178 at 32.556 kb on + strand, within GFF5178 at 32.557 kb on - strand, within GFF5178 at 32.557 kb on - strand, within GFF5178 at 32.557 kb on - strand, within GFF5178 at 32.557 kb on - strand, within GFF5178 at 32.697 kb on + strand, within GFF5178 at 32.697 kb on + strand, within GFF5178 at 32.698 kb on - strand, within GFF5178 at 32.698 kb on - strand, within GFF5178 at 32.698 kb on - strand, within GFF5178 at 32.727 kb on + strand at 32.727 kb on + strand at 32.728 kb on - strand at 33.224 kb on + strand, within GFF5179 at 33.224 kb on + strand, within GFF5179 at 33.225 kb on - strand, within GFF5179 at 33.225 kb on - strand, within GFF5179 at 33.225 kb on - strand, within GFF5179 at 33.225 kb on - strand, within GFF5179 at 33.311 kb on + strand at 33.311 kb on + strand at 33.311 kb on + strand at 33.311 kb on + strand at 33.311 kb on + strand at 33.312 kb on - strand at 33.333 kb on - strand at 33.333 kb on - strand at 33.333 kb on - strand at 33.395 kb on + strand at 33.461 kb on + strand, within GFF5180 at 33.461 kb on + strand, within GFF5180 at 33.461 kb on + strand, within GFF5180 at 33.461 kb on + strand, within GFF5180 at 33.462 kb on - strand, within GFF5180 at 33.462 kb on - strand, within GFF5180 at 33.606 kb on - strand, within GFF5180 at 33.849 kb on - strand, within GFF5180 at 33.849 kb on - strand, within GFF5180 at 33.900 kb on - strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.055 kb on + strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.056 kb on - strand, within GFF5180 at 34.227 kb on - strand, within GFF5180 at 34.367 kb on + strand at 34.367 kb on + strand at 34.368 kb on - strand at 34.368 kb on - strand at 34.402 kb on + strand at 34.402 kb on + strand at 34.403 kb on - strand at 34.403 kb on - strand at 34.609 kb on - strand, within GFF5181 at 34.609 kb on - strand, within GFF5181 at 34.772 kb on + strand at 34.773 kb on - strand at 34.905 kb on + strand at 34.905 kb on + strand at 34.929 kb on + strand at 34.949 kb on + strand at 34.950 kb on - strand at 35.005 kb on - strand at 35.005 kb on - strand at 35.005 kb on - strand at 35.007 kb on - strand at 35.027 kb on + strand at 35.133 kb on + strand at 35.133 kb on + strand at 35.134 kb on - strand at 35.134 kb on - strand at 35.134 kb on - strand at 35.134 kb on - strand at 35.417 kb on + strand at 35.417 kb on + strand at 35.418 kb on - strand at 35.418 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 32,469 + GFF5178 0.49 -0.9 32,469 + GFF5178 0.49 -0.1 32,469 + GFF5178 0.49 +0.9 32,469 + GFF5178 0.49 -0.6 32,469 + GFF5178 0.49 -0.7 32,469 + GFF5178 0.49 -0.4 32,470 - GFF5178 0.49 -0.1 32,470 - GFF5178 0.49 -0.2 32,470 - GFF5178 0.49 +0.2 32,470 - GFF5178 0.49 +0.0 32,470 - GFF5178 0.49 -0.1 32,471 + GFF5178 0.49 -1.2 32,471 + GFF5178 0.49 -0.5 32,471 + GFF5178 0.49 -0.3 32,471 + GFF5178 0.49 -0.4 32,471 + GFF5178 0.49 -1.1 32,471 + GFF5178 0.49 -0.5 32,471 + GFF5178 0.49 -0.8 32,471 + GFF5178 0.49 -0.3 32,471 + GFF5178 0.49 -0.8 32,471 + GFF5178 0.49 +0.1 32,471 + GFF5178 0.49 -0.5 32,471 + GFF5178 0.49 -1.4 32,471 + GFF5178 0.49 +0.6 32,471 + GFF5178 0.49 -0.7 32,471 + GFF5178 0.49 +1.0 32,471 + GFF5178 0.49 +0.1 32,472 - GFF5178 0.49 +0.1 32,472 - GFF5178 0.49 +2.9 32,472 - GFF5178 0.49 -0.2 32,556 + GFF5178 0.63 +1.2 32,556 + GFF5178 0.63 +0.2 32,556 + GFF5178 0.63 -0.3 32,556 + GFF5178 0.63 -0.1 32,557 - GFF5178 0.63 -0.4 32,557 - GFF5178 0.63 -0.0 32,557 - GFF5178 0.63 -0.1 32,557 - GFF5178 0.63 -1.7 32,697 + GFF5178 0.87 -0.7 32,697 + GFF5178 0.87 -1.2 32,698 - GFF5178 0.87 +0.8 32,698 - GFF5178 0.87 -0.6 32,698 - GFF5178 0.87 -0.3 32,727 + +1.0 32,727 + -0.4 32,728 - -0.1 33,224 + GFF5179 0.80 +0.1 33,224 + GFF5179 0.80 +0.4 33,225 - GFF5179 0.80 -0.4 33,225 - GFF5179 0.80 +0.2 33,225 - GFF5179 0.80 +0.1 33,225 - GFF5179 0.80 +0.4 33,311 + -0.2 33,311 + -0.2 33,311 + -1.7 33,311 + -2.1 33,311 + -0.7 33,312 - +0.1 33,333 - -0.5 33,333 - -0.5 33,333 - +0.3 33,395 + -0.5 33,461 + GFF5180 0.11 +0.9 33,461 + GFF5180 0.11 -0.1 33,461 + GFF5180 0.11 -0.3 33,461 + GFF5180 0.11 -0.5 33,462 - GFF5180 0.12 -1.0 33,462 - GFF5180 0.12 +0.1 33,606 - GFF5180 0.24 -0.4 33,849 - GFF5180 0.46 -0.1 33,849 - GFF5180 0.46 -1.8 33,900 - GFF5180 0.50 -0.8 34,055 + GFF5180 0.64 -0.3 34,055 + GFF5180 0.64 -0.0 34,055 + GFF5180 0.64 -0.9 34,055 + GFF5180 0.64 -0.5 34,055 + GFF5180 0.64 +0.2 34,055 + GFF5180 0.64 -0.8 34,055 + GFF5180 0.64 -1.8 34,055 + GFF5180 0.64 -0.6 34,055 + GFF5180 0.64 -1.3 34,056 - GFF5180 0.64 -2.1 34,056 - GFF5180 0.64 +0.0 34,056 - GFF5180 0.64 +0.0 34,056 - GFF5180 0.64 -1.0 34,056 - GFF5180 0.64 -0.4 34,056 - GFF5180 0.64 -0.4 34,056 - GFF5180 0.64 +1.4 34,056 - GFF5180 0.64 -0.4 34,227 - GFF5180 0.79 +0.0 34,367 + -0.5 34,367 + -1.4 34,368 - +0.2 34,368 - -1.0 34,402 + -0.5 34,402 + +0.0 34,403 - +0.3 34,403 - -0.6 34,609 - GFF5181 0.34 -0.4 34,609 - GFF5181 0.34 +0.6 34,772 + -0.1 34,773 - -0.5 34,905 + -0.2 34,905 + -0.2 34,929 + -0.1 34,949 + +0.0 34,950 - +0.5 35,005 - +0.2 35,005 - -1.3 35,005 - -0.2 35,007 - +0.1 35,027 + -0.4 35,133 + -0.8 35,133 + +0.2 35,134 - -0.4 35,134 - -0.3 35,134 - +0.0 35,134 - -0.8 35,417 + +0.8 35,417 + -0.2 35,418 - -0.1 35,418 - -0.9
Or see this region's nucleotide sequence