Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF515 and GFF516 are separated by 144 nucleotides GFF516 and GFF517 overlap by 4 nucleotides GFF517 and GFF518 are separated by 56 nucleotides GFF518 and GFF519 are separated by 145 nucleotides GFF519 and GFF520 overlap by 4 nucleotides
GFF515 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 554,973 to 556,160
GFF515
GFF516 - hypothetical protein, at 556,305 to 556,580
GFF516
GFF517 - no description, at 556,577 to 556,915
GFF517
GFF518 - Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains, at 556,972 to 558,504
GFF518
GFF519 - Transcriptional regulator, AcrR family, at 558,650 to 559,345
GFF519
GFF520 - Efflux transport system, outer membrane factor (OMF) lipoprotein, at 559,342 to 560,868
GFF520
Position (kb)
556
557
558
559 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 555.991 kb on + strand, within GFF515 at 555.991 kb on + strand, within GFF515 at 555.992 kb on - strand, within GFF515 at 556.034 kb on - strand, within GFF515 at 556.034 kb on - strand, within GFF515 at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.060 kb on + strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.061 kb on - strand at 556.071 kb on + strand at 556.072 kb on - strand at 556.213 kb on + strand at 556.214 kb on - strand at 556.224 kb on + strand at 556.225 kb on - strand at 556.306 kb on + strand at 556.306 kb on + strand at 556.307 kb on - strand at 556.310 kb on + strand at 556.311 kb on - strand at 556.311 kb on - strand at 556.311 kb on - strand at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.638 kb on + strand, within GFF517 at 556.639 kb on - strand, within GFF517 at 556.639 kb on - strand, within GFF517 at 556.639 kb on - strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.641 kb on + strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.642 kb on - strand, within GFF517 at 556.659 kb on + strand, within GFF517 at 556.659 kb on + strand, within GFF517 at 556.659 kb on + strand, within GFF517 at 556.659 kb on + strand, within GFF517 at 556.659 kb on + strand, within GFF517 at 556.660 kb on - strand, within GFF517 at 556.660 kb on - strand, within GFF517 at 556.660 kb on - strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.671 kb on + strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.672 kb on - strand, within GFF517 at 556.675 kb on + strand, within GFF517 at 556.675 kb on + strand, within GFF517 at 556.675 kb on + strand, within GFF517 at 556.675 kb on + strand, within GFF517 at 556.675 kb on + strand, within GFF517 at 556.675 kb on + strand, within GFF517 at 556.676 kb on - strand, within GFF517 at 556.676 kb on - strand, within GFF517 at 556.953 kb on - strand at 556.953 kb on - strand at 556.953 kb on - strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.000 kb on + strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.001 kb on - strand at 557.462 kb on + strand, within GFF518 at 557.462 kb on + strand, within GFF518 at 557.462 kb on + strand, within GFF518 at 557.463 kb on - strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.465 kb on + strand, within GFF518 at 557.466 kb on - strand, within GFF518 at 557.466 kb on - strand, within GFF518 at 557.957 kb on + strand, within GFF518 at 557.957 kb on + strand, within GFF518 at 557.957 kb on + strand, within GFF518 at 557.957 kb on + strand, within GFF518 at 557.957 kb on + strand, within GFF518 at 557.957 kb on + strand, within GFF518 at 557.958 kb on - strand, within GFF518 at 557.958 kb on - strand, within GFF518 at 557.958 kb on - strand, within GFF518 at 557.958 kb on - strand, within GFF518 at 557.993 kb on + strand, within GFF518 at 557.994 kb on - strand, within GFF518 at 558.364 kb on + strand at 558.364 kb on + strand at 558.364 kb on + strand at 558.364 kb on + strand at 558.365 kb on - strand at 558.365 kb on - strand at 558.515 kb on + strand at 558.516 kb on - strand at 558.516 kb on - strand at 558.582 kb on - strand at 558.606 kb on - strand at 558.606 kb on - strand at 558.606 kb on - strand at 558.639 kb on + strand at 558.639 kb on + strand at 558.827 kb on + strand, within GFF519 at 558.828 kb on - strand, within GFF519 at 558.828 kb on - strand, within GFF519 at 558.828 kb on - strand, within GFF519 at 558.830 kb on + strand, within GFF519 at 558.830 kb on + strand, within GFF519 at 558.830 kb on + strand, within GFF519 at 558.830 kb on + strand, within GFF519 at 558.830 kb on + strand, within GFF519 at 558.831 kb on - strand, within GFF519 at 558.831 kb on - strand, within GFF519 at 558.831 kb on - strand, within GFF519 at 558.831 kb on - strand, within GFF519 at 558.855 kb on - strand, within GFF519 at 558.949 kb on + strand, within GFF519 at 558.949 kb on + strand, within GFF519 at 558.950 kb on - strand, within GFF519 at 558.950 kb on - strand, within GFF519 at 558.950 kb on - strand, within GFF519 at 558.950 kb on - strand, within GFF519 at 558.950 kb on - strand, within GFF519 at 559.190 kb on + strand, within GFF519 at 559.191 kb on - strand, within GFF519 at 559.191 kb on - strand, within GFF519 at 559.191 kb on - strand, within GFF519 at 559.191 kb on - strand, within GFF519 at 559.191 kb on - strand, within GFF519 at 559.191 kb on - strand, within GFF519 at 559.191 kb on - strand, within GFF519
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 555,991 + GFF515 0.86 -1.4 555,991 + GFF515 0.86 +0.2 555,992 - GFF515 0.86 -2.7 556,034 - GFF515 0.89 -1.1 556,034 - GFF515 0.89 -0.5 556,060 + -0.7 556,060 + +0.9 556,060 + +1.8 556,060 + -1.1 556,060 + +0.2 556,060 + +2.8 556,060 + +0.6 556,060 + -0.4 556,060 + -0.0 556,060 + -0.1 556,061 - -0.2 556,061 - -1.3 556,061 - +0.2 556,061 - +0.3 556,061 - +1.2 556,061 - -0.1 556,061 - -0.1 556,061 - -1.1 556,061 - +0.5 556,061 - -0.4 556,061 - -0.7 556,071 + +0.4 556,072 - -0.2 556,213 + +0.5 556,214 - +0.1 556,224 + +0.2 556,225 - -0.3 556,306 + -0.0 556,306 + -0.7 556,307 - +0.3 556,310 + -0.8 556,311 - -0.4 556,311 - -0.9 556,311 - -1.2 556,638 + GFF517 0.18 +0.4 556,638 + GFF517 0.18 -0.5 556,638 + GFF517 0.18 -0.5 556,638 + GFF517 0.18 +0.1 556,638 + GFF517 0.18 -0.4 556,638 + GFF517 0.18 +0.4 556,638 + GFF517 0.18 +0.0 556,638 + GFF517 0.18 -1.5 556,638 + GFF517 0.18 -0.6 556,638 + GFF517 0.18 -0.2 556,639 - GFF517 0.18 -0.1 556,639 - GFF517 0.18 +0.5 556,639 - GFF517 0.18 +0.1 556,641 + GFF517 0.19 +0.4 556,641 + GFF517 0.19 +2.5 556,641 + GFF517 0.19 +0.9 556,641 + GFF517 0.19 -2.1 556,641 + GFF517 0.19 +0.7 556,641 + GFF517 0.19 -0.5 556,641 + GFF517 0.19 -0.1 556,641 + GFF517 0.19 +0.1 556,641 + GFF517 0.19 +1.0 556,641 + GFF517 0.19 +0.3 556,641 + GFF517 0.19 +0.4 556,641 + GFF517 0.19 -0.3 556,642 - GFF517 0.19 -0.8 556,642 - GFF517 0.19 -0.3 556,642 - GFF517 0.19 -1.2 556,642 - GFF517 0.19 -2.1 556,642 - GFF517 0.19 -0.7 556,642 - GFF517 0.19 -0.6 556,642 - GFF517 0.19 -0.7 556,642 - GFF517 0.19 -0.6 556,642 - GFF517 0.19 -0.2 556,642 - GFF517 0.19 -0.8 556,642 - GFF517 0.19 +0.5 556,642 - GFF517 0.19 -1.3 556,642 - GFF517 0.19 +0.5 556,642 - GFF517 0.19 -0.0 556,642 - GFF517 0.19 +0.5 556,642 - GFF517 0.19 -1.2 556,642 - GFF517 0.19 +0.2 556,659 + GFF517 0.24 +0.3 556,659 + GFF517 0.24 +0.0 556,659 + GFF517 0.24 -0.3 556,659 + GFF517 0.24 -0.8 556,659 + GFF517 0.24 -0.5 556,660 - GFF517 0.24 +0.3 556,660 - GFF517 0.24 -0.6 556,660 - GFF517 0.24 +1.6 556,671 + GFF517 0.28 -0.4 556,671 + GFF517 0.28 -0.3 556,671 + GFF517 0.28 -0.3 556,671 + GFF517 0.28 +0.2 556,671 + GFF517 0.28 -0.4 556,671 + GFF517 0.28 -0.4 556,671 + GFF517 0.28 -0.2 556,672 - GFF517 0.28 -0.7 556,672 - GFF517 0.28 +0.4 556,672 - GFF517 0.28 -0.8 556,672 - GFF517 0.28 +0.1 556,672 - GFF517 0.28 -2.3 556,672 - GFF517 0.28 -1.7 556,672 - GFF517 0.28 +0.6 556,675 + GFF517 0.29 +0.4 556,675 + GFF517 0.29 -0.3 556,675 + GFF517 0.29 -0.1 556,675 + GFF517 0.29 -0.8 556,675 + GFF517 0.29 +0.9 556,675 + GFF517 0.29 -0.8 556,676 - GFF517 0.29 -0.4 556,676 - GFF517 0.29 +1.0 556,953 - +0.0 556,953 - +0.5 556,953 - +0.1 557,000 + -2.0 557,000 + +0.0 557,000 + +0.2 557,000 + -1.9 557,000 + -1.2 557,000 + -0.5 557,000 + +0.2 557,000 + -0.0 557,000 + -0.7 557,001 - -0.5 557,001 - +0.1 557,001 - -0.3 557,001 - +0.4 557,001 - -1.8 557,001 - -0.3 557,001 - +0.5 557,001 - -0.7 557,001 - -0.7 557,001 - +0.6 557,001 - -0.4 557,001 - +1.2 557,462 + GFF518 0.32 -0.9 557,462 + GFF518 0.32 -0.0 557,462 + GFF518 0.32 +0.0 557,463 - GFF518 0.32 -0.2 557,465 + GFF518 0.32 -0.8 557,465 + GFF518 0.32 +2.2 557,465 + GFF518 0.32 -0.0 557,465 + GFF518 0.32 -0.0 557,465 + GFF518 0.32 -0.1 557,465 + GFF518 0.32 +0.4 557,465 + GFF518 0.32 -0.8 557,465 + GFF518 0.32 +0.2 557,465 + GFF518 0.32 -0.2 557,465 + GFF518 0.32 -1.6 557,466 - GFF518 0.32 -0.4 557,466 - GFF518 0.32 +0.2 557,957 + GFF518 0.64 +0.3 557,957 + GFF518 0.64 +0.9 557,957 + GFF518 0.64 +0.3 557,957 + GFF518 0.64 -0.6 557,957 + GFF518 0.64 +0.4 557,957 + GFF518 0.64 +0.1 557,958 - GFF518 0.64 +1.3 557,958 - GFF518 0.64 +0.8 557,958 - GFF518 0.64 -0.7 557,958 - GFF518 0.64 +2.0 557,993 + GFF518 0.67 -0.5 557,994 - GFF518 0.67 -2.0 558,364 + +0.5 558,364 + +0.3 558,364 + -1.6 558,364 + -0.5 558,365 - -0.1 558,365 - +0.2 558,515 + +1.0 558,516 - -0.3 558,516 - -0.2 558,582 - +0.6 558,606 - -0.5 558,606 - -0.6 558,606 - -0.0 558,639 + +0.6 558,639 + +0.2 558,827 + GFF519 0.25 +0.4 558,828 - GFF519 0.26 +0.9 558,828 - GFF519 0.26 -0.2 558,828 - GFF519 0.26 +0.4 558,830 + GFF519 0.26 -0.3 558,830 + GFF519 0.26 +0.2 558,830 + GFF519 0.26 -0.4 558,830 + GFF519 0.26 -0.2 558,830 + GFF519 0.26 +0.1 558,831 - GFF519 0.26 +0.6 558,831 - GFF519 0.26 +1.5 558,831 - GFF519 0.26 +0.6 558,831 - GFF519 0.26 -0.0 558,855 - GFF519 0.29 -1.7 558,949 + GFF519 0.43 -0.8 558,949 + GFF519 0.43 -0.1 558,950 - GFF519 0.43 +0.1 558,950 - GFF519 0.43 +0.9 558,950 - GFF519 0.43 -0.2 558,950 - GFF519 0.43 -2.0 558,950 - GFF519 0.43 -2.0 559,190 + GFF519 0.78 -1.3 559,191 - GFF519 0.78 -0.4 559,191 - GFF519 0.78 -2.2 559,191 - GFF519 0.78 -0.6 559,191 - GFF519 0.78 -0.3 559,191 - GFF519 0.78 -0.6 559,191 - GFF519 0.78 +1.2 559,191 - GFF519 0.78 -0.5
Or see this region's nucleotide sequence