Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5157 and GFF5158 overlap by 4 nucleotides GFF5158 and GFF5159 are separated by 58 nucleotides
GFF5157 - Glucoamylase (EC 3.2.1.3), at 9,614 to 11,482
GFF5157
GFF5158 - Trehalose-6-phosphate phosphatase (EC 3.1.3.12), at 11,479 to 12,297
GFF5158
GFF5159 - CCA tRNA nucleotidyltransferase (EC 2.7.7.72), at 12,356 to 13,615
GFF5159
Position (kb)
11
12
13 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2 at 10.479 kb on + strand, within GFF5157 at 10.479 kb on + strand, within GFF5157 at 10.479 kb on + strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.480 kb on - strand, within GFF5157 at 10.539 kb on + strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.540 kb on - strand, within GFF5157 at 10.614 kb on + strand, within GFF5157 at 10.615 kb on - strand, within GFF5157 at 10.620 kb on + strand, within GFF5157 at 10.620 kb on + strand, within GFF5157 at 10.620 kb on + strand, within GFF5157 at 10.621 kb on - strand, within GFF5157 at 10.621 kb on - strand, within GFF5157 at 10.621 kb on - strand, within GFF5157 at 10.698 kb on + strand, within GFF5157 at 10.699 kb on - strand, within GFF5157 at 10.699 kb on - strand, within GFF5157 at 10.746 kb on + strand, within GFF5157 at 10.747 kb on - strand, within GFF5157 at 10.747 kb on - strand, within GFF5157 at 10.747 kb on - strand, within GFF5157 at 10.789 kb on - strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.872 kb on + strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.873 kb on - strand, within GFF5157 at 10.962 kb on + strand, within GFF5157 at 10.962 kb on + strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 10.963 kb on - strand, within GFF5157 at 11.115 kb on + strand, within GFF5157 at 11.115 kb on + strand, within GFF5157 at 11.115 kb on + strand, within GFF5157 at 11.116 kb on - strand, within GFF5157 at 11.116 kb on - strand, within GFF5157 at 11.116 kb on - strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.202 kb on + strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.203 kb on - strand, within GFF5157 at 11.355 kb on + strand at 11.355 kb on + strand at 11.355 kb on + strand at 11.355 kb on + strand at 11.355 kb on + strand at 11.356 kb on - strand at 11.356 kb on - strand at 11.567 kb on + strand, within GFF5158 at 11.567 kb on + strand, within GFF5158 at 11.567 kb on + strand, within GFF5158 at 11.568 kb on - strand, within GFF5158 at 11.568 kb on - strand, within GFF5158 at 12.201 kb on + strand, within GFF5158 at 12.201 kb on + strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.202 kb on - strand, within GFF5158 at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.292 kb on + strand at 12.293 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 10,479 + GFF5157 0.46 -0.5 10,479 + GFF5157 0.46 -0.2 10,479 + GFF5157 0.46 +2.0 10,480 - GFF5157 0.46 +1.4 10,480 - GFF5157 0.46 -0.5 10,480 - GFF5157 0.46 -0.1 10,480 - GFF5157 0.46 -1.4 10,480 - GFF5157 0.46 +0.3 10,539 + GFF5157 0.49 -1.4 10,540 - GFF5157 0.50 +0.6 10,540 - GFF5157 0.50 -0.7 10,540 - GFF5157 0.50 -0.2 10,540 - GFF5157 0.50 +0.0 10,540 - GFF5157 0.50 -1.0 10,540 - GFF5157 0.50 -0.5 10,540 - GFF5157 0.50 -0.5 10,614 + GFF5157 0.54 -0.2 10,615 - GFF5157 0.54 +0.0 10,620 + GFF5157 0.54 -0.3 10,620 + GFF5157 0.54 -1.2 10,620 + GFF5157 0.54 -0.6 10,621 - GFF5157 0.54 -0.3 10,621 - GFF5157 0.54 -0.8 10,621 - GFF5157 0.54 -0.4 10,698 + GFF5157 0.58 +0.1 10,699 - GFF5157 0.58 +0.6 10,699 - GFF5157 0.58 +0.0 10,746 + GFF5157 0.61 -0.2 10,747 - GFF5157 0.61 -1.4 10,747 - GFF5157 0.61 +0.6 10,747 - GFF5157 0.61 -0.4 10,789 - GFF5157 0.63 +0.6 10,872 + GFF5157 0.67 +1.0 10,872 + GFF5157 0.67 -0.4 10,872 + GFF5157 0.67 -1.7 10,872 + GFF5157 0.67 -0.1 10,872 + GFF5157 0.67 -0.8 10,873 - GFF5157 0.67 -0.4 10,873 - GFF5157 0.67 +0.1 10,873 - GFF5157 0.67 -0.1 10,873 - GFF5157 0.67 -1.0 10,873 - GFF5157 0.67 -0.3 10,873 - GFF5157 0.67 -0.4 10,873 - GFF5157 0.67 -0.6 10,962 + GFF5157 0.72 +0.1 10,962 + GFF5157 0.72 +0.2 10,963 - GFF5157 0.72 +0.0 10,963 - GFF5157 0.72 -0.3 10,963 - GFF5157 0.72 +0.2 10,963 - GFF5157 0.72 -0.5 11,115 + GFF5157 0.80 +0.0 11,115 + GFF5157 0.80 -0.5 11,115 + GFF5157 0.80 +0.2 11,116 - GFF5157 0.80 -0.7 11,116 - GFF5157 0.80 -0.8 11,116 - GFF5157 0.80 +2.1 11,202 + GFF5157 0.85 -0.9 11,202 + GFF5157 0.85 -0.7 11,202 + GFF5157 0.85 -0.5 11,202 + GFF5157 0.85 -0.4 11,202 + GFF5157 0.85 -1.7 11,202 + GFF5157 0.85 +0.7 11,202 + GFF5157 0.85 -0.7 11,202 + GFF5157 0.85 -3.0 11,203 - GFF5157 0.85 +0.1 11,203 - GFF5157 0.85 -0.2 11,203 - GFF5157 0.85 -1.1 11,203 - GFF5157 0.85 -0.5 11,203 - GFF5157 0.85 -2.1 11,355 + +0.4 11,355 + +0.1 11,355 + -0.4 11,355 + -0.2 11,355 + -0.7 11,356 - +0.6 11,356 - +1.4 11,567 + GFF5158 0.11 -1.4 11,567 + GFF5158 0.11 -2.9 11,567 + GFF5158 0.11 -1.7 11,568 - GFF5158 0.11 -5.0 11,568 - GFF5158 0.11 -3.8 12,201 + GFF5158 0.88 -2.2 12,201 + GFF5158 0.88 -3.4 12,202 - GFF5158 0.88 -2.3 12,202 - GFF5158 0.88 -3.7 12,202 - GFF5158 0.88 -5.7 12,202 - GFF5158 0.88 -1.4 12,292 + -0.7 12,292 + +0.1 12,292 + +0.1 12,292 + -0.4 12,292 + -1.2 12,292 + -0.0 12,293 - +0.4
Or see this region's nucleotide sequence