Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5004 and GFF5005 are separated by 85 nucleotides GFF5005 and GFF5006 are separated by 134 nucleotides GFF5006 and GFF5007 are separated by 31 nucleotides
GFF5004 - N-acyl-L-amino acid amidohydrolase (EC 3.5.1.14), at 103,833 to 105,035
GFF5004
GFF5005 - cytidine/deoxycytidylate deaminase family protein, at 105,121 to 105,651
GFF5005
GFF5006 - Uncharacterized deacetylase, at 105,786 to 106,952
GFF5006
GFF5007 - probable membrane protein STY4873, at 106,984 to 108,207
GFF5007
Position (kb)
105
106
107 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 105.043 kb on - strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.062 kb on + strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.063 kb on - strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.064 kb on + strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.065 kb on - strand at 105.091 kb on + strand at 105.091 kb on + strand at 105.091 kb on + strand at 105.091 kb on + strand at 105.092 kb on - strand at 105.092 kb on - strand at 105.092 kb on - strand at 105.095 kb on + strand at 105.095 kb on + strand at 105.095 kb on + strand at 105.096 kb on - strand at 105.096 kb on - strand at 105.096 kb on - strand at 105.096 kb on - strand at 105.299 kb on + strand, within GFF5005 at 105.299 kb on + strand, within GFF5005 at 105.300 kb on - strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.326 kb on + strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.327 kb on - strand, within GFF5005 at 105.563 kb on - strand, within GFF5005 at 105.563 kb on - strand, within GFF5005 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.930 kb on + strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 105.931 kb on - strand, within GFF5006 at 106.010 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.194 kb on + strand, within GFF5006 at 106.195 kb on - strand, within GFF5006 at 106.195 kb on - strand, within GFF5006 at 106.326 kb on + strand, within GFF5006 at 106.327 kb on - strand, within GFF5006 at 106.327 kb on - strand, within GFF5006 at 106.327 kb on - strand, within GFF5006 at 106.327 kb on - strand, within GFF5006 at 106.333 kb on - strand, within GFF5006 at 106.333 kb on - strand, within GFF5006 at 106.333 kb on - strand, within GFF5006 at 106.467 kb on + strand, within GFF5006 at 106.564 kb on - strand, within GFF5006 at 106.623 kb on + strand, within GFF5006 at 106.623 kb on + strand, within GFF5006 at 106.623 kb on + strand, within GFF5006 at 106.623 kb on + strand, within GFF5006 at 106.624 kb on - strand, within GFF5006 at 106.624 kb on - strand, within GFF5006 at 106.833 kb on - strand, within GFF5006 at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.029 kb on + strand at 107.030 kb on - strand at 107.030 kb on - strand at 107.030 kb on - strand at 107.030 kb on - strand at 107.030 kb on - strand at 107.030 kb on - strand at 107.074 kb on + strand at 107.074 kb on + strand at 107.074 kb on + strand at 107.074 kb on + strand at 107.075 kb on - strand at 107.075 kb on - strand at 107.461 kb on - strand, within GFF5007 at 107.611 kb on + strand, within GFF5007 at 107.611 kb on + strand, within GFF5007 at 107.611 kb on + strand, within GFF5007 at 107.612 kb on - strand, within GFF5007 at 107.612 kb on - strand, within GFF5007 at 107.612 kb on - strand, within GFF5007 at 107.612 kb on - strand, within GFF5007 at 107.836 kb on + strand, within GFF5007 at 107.836 kb on + strand, within GFF5007 at 107.836 kb on + strand, within GFF5007 at 107.837 kb on - strand, within GFF5007 at 107.837 kb on - strand, within GFF5007 at 107.837 kb on - strand, within GFF5007
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 105,043 - -0.4 105,062 + -0.8 105,062 + +1.1 105,062 + -0.6 105,062 + -0.8 105,062 + +0.5 105,062 + -1.1 105,062 + +0.1 105,062 + +0.6 105,062 + +0.1 105,062 + -1.7 105,062 + -0.8 105,063 - -0.4 105,063 - -0.5 105,063 - +1.6 105,063 - -0.8 105,063 - +0.2 105,063 - -0.2 105,063 - +1.7 105,063 - -0.9 105,063 - +3.7 105,063 - +0.3 105,064 + -0.8 105,064 + -1.5 105,064 + -0.6 105,064 + -0.1 105,064 + +0.7 105,064 + -0.2 105,064 + -0.2 105,064 + -2.1 105,064 + -1.1 105,064 + +0.0 105,064 + +1.7 105,064 + +0.5 105,065 - +1.0 105,065 - +0.6 105,065 - +0.7 105,065 - -0.7 105,065 - +0.9 105,065 - +0.2 105,065 - +0.3 105,065 - -0.5 105,065 - +0.1 105,065 - -1.1 105,065 - -0.8 105,065 - -0.3 105,065 - -0.1 105,065 - -0.7 105,065 - +0.9 105,065 - -0.4 105,065 - +0.3 105,091 + -0.2 105,091 + -0.2 105,091 + +0.6 105,091 + -0.6 105,092 - +0.0 105,092 - +0.4 105,092 - -0.5 105,095 + -1.6 105,095 + -0.4 105,095 + -0.0 105,096 - +0.1 105,096 - -0.1 105,096 - +0.2 105,096 - +0.0 105,299 + GFF5005 0.34 -0.4 105,299 + GFF5005 0.34 -0.5 105,300 - GFF5005 0.34 +0.4 105,326 + GFF5005 0.39 -0.9 105,326 + GFF5005 0.39 -1.3 105,326 + GFF5005 0.39 -0.5 105,326 + GFF5005 0.39 -0.3 105,326 + GFF5005 0.39 +1.0 105,326 + GFF5005 0.39 -0.7 105,326 + GFF5005 0.39 -0.5 105,326 + GFF5005 0.39 -0.5 105,326 + GFF5005 0.39 -1.7 105,327 - GFF5005 0.39 -2.5 105,327 - GFF5005 0.39 +0.1 105,327 - GFF5005 0.39 -1.7 105,327 - GFF5005 0.39 -0.8 105,327 - GFF5005 0.39 +0.7 105,327 - GFF5005 0.39 -1.1 105,327 - GFF5005 0.39 -2.0 105,327 - GFF5005 0.39 +2.3 105,327 - GFF5005 0.39 +0.3 105,327 - GFF5005 0.39 -0.3 105,327 - GFF5005 0.39 -0.2 105,327 - GFF5005 0.39 +0.2 105,563 - GFF5005 0.83 +0.0 105,563 - GFF5005 0.83 -0.0 105,930 + GFF5006 0.12 +0.2 105,930 + GFF5006 0.12 -0.0 105,930 + GFF5006 0.12 +1.1 105,930 + GFF5006 0.12 +0.4 105,930 + GFF5006 0.12 +0.2 105,930 + GFF5006 0.12 -0.3 105,930 + GFF5006 0.12 +0.1 105,930 + GFF5006 0.12 +0.2 105,930 + GFF5006 0.12 +0.7 105,930 + GFF5006 0.12 +0.0 105,931 - GFF5006 0.12 +0.2 105,931 - GFF5006 0.12 -0.1 105,931 - GFF5006 0.12 +1.5 105,931 - GFF5006 0.12 -0.7 105,931 - GFF5006 0.12 +0.0 105,931 - GFF5006 0.12 +0.2 105,931 - GFF5006 0.12 +0.2 105,931 - GFF5006 0.12 -0.3 105,931 - GFF5006 0.12 -0.1 105,931 - GFF5006 0.12 -0.1 105,931 - GFF5006 0.12 +0.7 105,931 - GFF5006 0.12 +0.3 106,010 + GFF5006 0.19 -0.2 106,194 + GFF5006 0.35 +0.6 106,194 + GFF5006 0.35 +0.3 106,194 + GFF5006 0.35 +0.6 106,194 + GFF5006 0.35 -1.0 106,194 + GFF5006 0.35 -0.2 106,194 + GFF5006 0.35 -0.1 106,194 + GFF5006 0.35 -0.4 106,194 + GFF5006 0.35 +0.9 106,194 + GFF5006 0.35 +1.7 106,195 - GFF5006 0.35 -1.3 106,195 - GFF5006 0.35 +0.4 106,326 + GFF5006 0.46 +0.2 106,327 - GFF5006 0.46 +0.0 106,327 - GFF5006 0.46 -0.1 106,327 - GFF5006 0.46 +0.3 106,327 - GFF5006 0.46 +0.4 106,333 - GFF5006 0.47 -0.4 106,333 - GFF5006 0.47 -0.3 106,333 - GFF5006 0.47 +0.5 106,467 + GFF5006 0.58 -1.2 106,564 - GFF5006 0.67 +0.7 106,623 + GFF5006 0.72 -0.6 106,623 + GFF5006 0.72 +0.2 106,623 + GFF5006 0.72 -0.1 106,623 + GFF5006 0.72 -1.3 106,624 - GFF5006 0.72 +0.6 106,624 - GFF5006 0.72 +1.2 106,833 - GFF5006 0.90 -0.5 107,029 + +0.0 107,029 + -0.5 107,029 + -0.1 107,029 + -1.6 107,029 + +0.4 107,029 + -0.8 107,029 + +1.7 107,029 + -0.5 107,029 + -1.3 107,029 + -1.0 107,030 - +0.1 107,030 - +0.6 107,030 - -1.4 107,030 - -0.1 107,030 - -0.4 107,030 - -0.1 107,074 + -0.4 107,074 + +1.7 107,074 + -0.3 107,074 + +0.1 107,075 - -0.5 107,075 - -0.1 107,461 - GFF5007 0.39 +0.1 107,611 + GFF5007 0.51 +0.6 107,611 + GFF5007 0.51 -0.9 107,611 + GFF5007 0.51 +0.4 107,612 - GFF5007 0.51 -0.6 107,612 - GFF5007 0.51 -0.0 107,612 - GFF5007 0.51 -1.5 107,612 - GFF5007 0.51 -1.1 107,836 + GFF5007 0.70 +0.2 107,836 + GFF5007 0.70 -2.6 107,836 + GFF5007 0.70 +0.3 107,837 - GFF5007 0.70 -1.3 107,837 - GFF5007 0.70 +0.5 107,837 - GFF5007 0.70 -0.4
Or see this region's nucleotide sequence