Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF498 and GFF499 overlap by 4 nucleotides GFF499 and GFF500 are separated by 16 nucleotides GFF500 and GFF501 are separated by 119 nucleotides GFF501 and GFF502 are separated by 4 nucleotides
GFF498 - Squalene/phytoene desaturase HopC, at 542,122 to 543,378
GFF498
GFF499 - hypothetical protein, at 543,375 to 543,683
GFF499
GFF500 - Lysine exporter protein (LYSE/YGGA), at 543,700 to 544,344
GFF500
GFF501 - hypothetical protein, at 544,464 to 544,769
GFF501
GFF502 - Hydrolase, at 544,774 to 545,505
GFF502
Position (kb)
543
544
545 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 542.980 kb on + strand, within GFF498 at 542.980 kb on + strand, within GFF498 at 542.980 kb on + strand, within GFF498 at 542.980 kb on + strand, within GFF498 at 542.980 kb on + strand, within GFF498 at 542.981 kb on - strand, within GFF498 at 542.981 kb on - strand, within GFF498 at 542.981 kb on - strand, within GFF498 at 542.981 kb on - strand, within GFF498 at 543.095 kb on + strand, within GFF498 at 543.298 kb on + strand at 543.393 kb on + strand at 543.394 kb on - strand at 543.394 kb on - strand at 543.409 kb on - strand, within GFF499 at 543.409 kb on - strand, within GFF499 at 543.481 kb on + strand, within GFF499 at 543.482 kb on - strand, within GFF499 at 543.482 kb on - strand, within GFF499 at 543.482 kb on - strand, within GFF499 at 543.482 kb on - strand, within GFF499 at 543.570 kb on + strand, within GFF499 at 543.570 kb on + strand, within GFF499 at 543.570 kb on + strand, within GFF499 at 543.571 kb on - strand, within GFF499 at 543.571 kb on - strand, within GFF499 at 543.571 kb on - strand, within GFF499 at 543.571 kb on - strand, within GFF499 at 543.836 kb on + strand, within GFF500 at 543.836 kb on + strand, within GFF500 at 543.836 kb on + strand, within GFF500 at 543.836 kb on + strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 543.837 kb on - strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.091 kb on + strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.092 kb on - strand, within GFF500 at 544.100 kb on + strand, within GFF500 at 544.101 kb on - strand, within GFF500 at 544.101 kb on - strand, within GFF500 at 544.101 kb on - strand, within GFF500 at 544.397 kb on + strand at 544.397 kb on + strand at 544.397 kb on + strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.398 kb on - strand at 544.399 kb on + strand at 544.400 kb on - strand at 544.437 kb on - strand at 544.477 kb on - strand at 544.477 kb on - strand at 544.477 kb on - strand at 544.477 kb on - strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.485 kb on + strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.486 kb on - strand at 544.500 kb on + strand, within GFF501 at 544.500 kb on + strand, within GFF501 at 544.501 kb on - strand, within GFF501 at 544.501 kb on - strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.578 kb on + strand, within GFF501 at 544.579 kb on - strand, within GFF501 at 544.704 kb on + strand, within GFF501 at 544.734 kb on + strand, within GFF501 at 544.734 kb on + strand, within GFF501 at 544.735 kb on - strand, within GFF501 at 544.735 kb on - strand, within GFF501 at 544.747 kb on - strand at 544.767 kb on + strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 544.768 kb on - strand at 545.141 kb on + strand, within GFF502 at 545.141 kb on + strand, within GFF502
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 542,980 + GFF498 0.68 -1.5 542,980 + GFF498 0.68 -0.4 542,980 + GFF498 0.68 -1.6 542,980 + GFF498 0.68 -0.4 542,980 + GFF498 0.68 +1.5 542,981 - GFF498 0.68 +0.5 542,981 - GFF498 0.68 -0.8 542,981 - GFF498 0.68 +0.2 542,981 - GFF498 0.68 -0.2 543,095 + GFF498 0.77 -1.7 543,298 + -0.3 543,393 + -0.1 543,394 - +0.7 543,394 - -1.1 543,409 - GFF499 0.11 +0.7 543,409 - GFF499 0.11 +0.5 543,481 + GFF499 0.34 -0.8 543,482 - GFF499 0.35 -0.8 543,482 - GFF499 0.35 -0.6 543,482 - GFF499 0.35 -0.1 543,482 - GFF499 0.35 -0.3 543,570 + GFF499 0.63 +1.9 543,570 + GFF499 0.63 -1.0 543,570 + GFF499 0.63 +0.2 543,571 - GFF499 0.63 -0.6 543,571 - GFF499 0.63 -2.7 543,571 - GFF499 0.63 +0.4 543,571 - GFF499 0.63 -1.8 543,836 + GFF500 0.21 -0.8 543,836 + GFF500 0.21 -0.4 543,836 + GFF500 0.21 -0.4 543,836 + GFF500 0.21 -0.4 543,837 - GFF500 0.21 -1.6 543,837 - GFF500 0.21 +0.0 543,837 - GFF500 0.21 +0.3 543,837 - GFF500 0.21 +1.2 543,837 - GFF500 0.21 -1.3 544,091 + GFF500 0.61 -1.0 544,091 + GFF500 0.61 -0.4 544,091 + GFF500 0.61 -0.6 544,091 + GFF500 0.61 -0.4 544,092 - GFF500 0.61 -1.0 544,092 - GFF500 0.61 -1.8 544,092 - GFF500 0.61 -0.2 544,092 - GFF500 0.61 -0.2 544,092 - GFF500 0.61 -0.6 544,100 + GFF500 0.62 -1.3 544,101 - GFF500 0.62 -0.9 544,101 - GFF500 0.62 +0.2 544,101 - GFF500 0.62 -0.5 544,397 + +0.1 544,397 + +0.5 544,397 + -0.9 544,398 - +0.1 544,398 - +1.2 544,398 - -0.0 544,398 - -1.1 544,398 - -0.2 544,398 - -0.2 544,398 - -1.4 544,399 + -0.8 544,400 - -0.5 544,437 - -0.7 544,477 - -0.4 544,477 - -0.1 544,477 - -1.7 544,477 - -0.9 544,485 + +0.8 544,485 + +0.8 544,485 + -1.0 544,485 + -0.5 544,485 + -0.1 544,485 + +0.3 544,486 - -2.3 544,486 - -0.7 544,486 - +0.1 544,486 - -0.9 544,486 - -0.2 544,486 - -0.1 544,500 + GFF501 0.12 +1.5 544,500 + GFF501 0.12 +0.1 544,501 - GFF501 0.12 -1.7 544,501 - GFF501 0.12 -0.3 544,578 + GFF501 0.37 +0.4 544,578 + GFF501 0.37 +1.8 544,578 + GFF501 0.37 +0.2 544,579 - GFF501 0.38 -0.4 544,704 + GFF501 0.78 -2.0 544,734 + GFF501 0.88 +0.4 544,734 + GFF501 0.88 +0.1 544,735 - GFF501 0.89 -0.4 544,735 - GFF501 0.89 -0.2 544,747 - -0.3 544,767 + -0.8 544,768 - -0.7 544,768 - -1.7 544,768 - -0.5 544,768 - -0.9 544,768 - -0.1 544,768 - +0.5 544,768 - +1.1 544,768 - -0.5 544,768 - +0.0 545,141 + GFF502 0.50 -0.4 545,141 + GFF502 0.50 -0.2
Or see this region's nucleotide sequence