Strain Fitness in Variovorax sp. SCN45 around GFF4959

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4958 and GFF4959 overlap by 4 nucleotidesGFF4959 and GFF4960 overlap by 4 nucleotidesGFF4960 and GFF4961 overlap by 4 nucleotides GFF4958 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 58,886 to 59,806 GFF4958 GFF4959 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 59,803 to 60,792 GFF4959 GFF4960 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 60,789 to 61,544 GFF4960 GFF4961 - ABC transporter, ATP-binding protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 61,541 to 62,260 GFF4961 Position (kb) 59 60 61Strain fitness (log2 ratio) -2 -1 0 1 2at 58.885 kb on + strandat 58.886 kb on - strandat 58.886 kb on - strandat 58.886 kb on - strandat 59.361 kb on - strand, within GFF4958at 59.556 kb on + strand, within GFF4958at 59.557 kb on - strand, within GFF4958at 59.557 kb on - strand, within GFF4958at 59.557 kb on - strand, within GFF4958at 59.744 kb on + strandat 59.745 kb on - strandat 59.745 kb on - strandat 59.745 kb on - strandat 59.745 kb on - strandat 59.824 kb on + strandat 59.824 kb on + strandat 59.825 kb on - strandat 59.899 kb on + strandat 59.899 kb on + strandat 59.899 kb on + strandat 60.019 kb on + strand, within GFF4959at 60.145 kb on + strand, within GFF4959at 60.146 kb on - strand, within GFF4959at 60.184 kb on + strand, within GFF4959at 60.185 kb on - strand, within GFF4959at 60.230 kb on - strand, within GFF4959

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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58,885 + -0.1
58,886 - +2.3
58,886 - +0.2
58,886 - -0.6
59,361 - GFF4958 0.52 -1.2
59,556 + GFF4958 0.73 +0.2
59,557 - GFF4958 0.73 -0.3
59,557 - GFF4958 0.73 -0.3
59,557 - GFF4958 0.73 -0.1
59,744 + -0.6
59,745 - -1.0
59,745 - +0.1
59,745 - +0.6
59,745 - -2.5
59,824 + -0.6
59,824 + -1.5
59,825 - -1.1
59,899 + -0.6
59,899 + +0.7
59,899 + -1.0
60,019 + GFF4959 0.22 +1.0
60,145 + GFF4959 0.35 -0.4
60,146 - GFF4959 0.35 -0.2
60,184 + GFF4959 0.38 +0.3
60,185 - GFF4959 0.39 +0.9
60,230 - GFF4959 0.43 -0.6

Or see this region's nucleotide sequence