Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4947 and GFF4948 are separated by 180 nucleotides GFF4948 and GFF4949 overlap by 4 nucleotides
GFF4947 - Erythritol kinase EryA (EC 2.7.1.27), at 42,178 to 43,665
GFF4947
GFF4948 - ABC transporter, substrate-binding protein (cluster 2, ribose/xylose/arabinose/galactose), at 43,846 to 44,892
GFF4948
GFF4949 - ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose) / ABC transporter, ATP-binding protein (cluster 2, ribose/xylose/arabinose/galactose), at 44,889 to 46,412
GFF4949
Position (kb)
43
44
45 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 43.175 kb on + strand, within GFF4947 at 43.175 kb on + strand, within GFF4947 at 43.175 kb on + strand, within GFF4947 at 43.175 kb on + strand, within GFF4947 at 43.176 kb on - strand, within GFF4947 at 43.403 kb on + strand, within GFF4947 at 43.403 kb on + strand, within GFF4947 at 43.403 kb on + strand, within GFF4947 at 43.403 kb on + strand, within GFF4947 at 43.403 kb on + strand, within GFF4947 at 43.404 kb on - strand, within GFF4947 at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.720 kb on + strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.721 kb on - strand at 43.743 kb on + strand at 43.743 kb on + strand at 43.744 kb on - strand at 43.855 kb on - strand at 43.975 kb on + strand, within GFF4948 at 43.976 kb on - strand, within GFF4948 at 43.976 kb on - strand, within GFF4948 at 44.008 kb on + strand, within GFF4948 at 44.009 kb on - strand, within GFF4948 at 44.009 kb on - strand, within GFF4948 at 44.023 kb on + strand, within GFF4948 at 44.024 kb on - strand, within GFF4948 at 44.090 kb on - strand, within GFF4948 at 44.479 kb on + strand, within GFF4948 at 44.480 kb on - strand, within GFF4948 at 44.692 kb on + strand, within GFF4948 at 44.692 kb on + strand, within GFF4948 at 44.692 kb on + strand, within GFF4948 at 44.692 kb on + strand, within GFF4948 at 44.692 kb on + strand, within GFF4948 at 44.693 kb on - strand, within GFF4948 at 44.693 kb on - strand, within GFF4948 at 44.693 kb on - strand, within GFF4948 at 44.693 kb on - strand, within GFF4948 at 44.693 kb on - strand, within GFF4948 at 44.693 kb on - strand, within GFF4948 at 44.842 kb on + strand at 44.854 kb on + strand at 44.855 kb on - strand at 44.855 kb on - strand at 44.855 kb on - strand at 44.855 kb on - strand at 45.241 kb on - strand, within GFF4949 at 45.483 kb on + strand, within GFF4949 at 45.483 kb on + strand, within GFF4949 at 45.484 kb on - strand, within GFF4949 at 45.484 kb on - strand, within GFF4949 at 45.484 kb on - strand, within GFF4949 at 45.509 kb on - strand, within GFF4949 at 45.630 kb on + strand, within GFF4949 at 45.630 kb on + strand, within GFF4949 at 45.630 kb on + strand, within GFF4949 at 45.630 kb on + strand, within GFF4949 at 45.630 kb on + strand, within GFF4949 at 45.631 kb on - strand, within GFF4949 at 45.631 kb on - strand, within GFF4949 at 45.631 kb on - strand, within GFF4949 at 45.631 kb on - strand, within GFF4949 at 45.631 kb on - strand, within GFF4949 at 45.631 kb on - strand, within GFF4949 at 45.634 kb on - strand, within GFF4949 at 45.634 kb on - strand, within GFF4949 at 45.867 kb on + strand, within GFF4949 at 45.867 kb on + strand, within GFF4949 at 45.868 kb on - strand, within GFF4949
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 43,175 + GFF4947 0.67 +0.2 43,175 + GFF4947 0.67 -0.4 43,175 + GFF4947 0.67 -0.5 43,175 + GFF4947 0.67 -0.4 43,176 - GFF4947 0.67 +1.0 43,403 + GFF4947 0.82 -1.4 43,403 + GFF4947 0.82 -0.2 43,403 + GFF4947 0.82 +0.4 43,403 + GFF4947 0.82 +0.0 43,403 + GFF4947 0.82 -0.8 43,404 - GFF4947 0.82 -1.3 43,720 + +0.7 43,720 + -0.2 43,720 + -0.6 43,720 + -0.6 43,720 + -1.1 43,720 + +0.2 43,720 + +0.4 43,720 + +0.6 43,720 + -0.6 43,720 + -1.2 43,721 - -0.2 43,721 - +1.5 43,721 - -1.1 43,721 - +0.2 43,721 - -0.1 43,721 - -1.7 43,721 - +0.3 43,721 - -0.4 43,721 - -0.2 43,721 - -0.3 43,721 - -0.4 43,721 - +2.1 43,721 - -0.1 43,743 + -0.2 43,743 + -0.9 43,744 - +0.2 43,855 - -0.5 43,975 + GFF4948 0.12 +0.2 43,976 - GFF4948 0.12 -0.1 43,976 - GFF4948 0.12 +0.2 44,008 + GFF4948 0.15 -1.2 44,009 - GFF4948 0.16 -1.4 44,009 - GFF4948 0.16 -0.2 44,023 + GFF4948 0.17 -0.3 44,024 - GFF4948 0.17 -0.7 44,090 - GFF4948 0.23 -0.4 44,479 + GFF4948 0.60 -0.8 44,480 - GFF4948 0.61 +1.0 44,692 + GFF4948 0.81 +0.2 44,692 + GFF4948 0.81 +0.2 44,692 + GFF4948 0.81 -1.1 44,692 + GFF4948 0.81 -0.2 44,692 + GFF4948 0.81 -0.3 44,693 - GFF4948 0.81 -0.4 44,693 - GFF4948 0.81 -1.3 44,693 - GFF4948 0.81 +0.1 44,693 - GFF4948 0.81 -1.6 44,693 - GFF4948 0.81 -1.3 44,693 - GFF4948 0.81 +0.1 44,842 + +1.1 44,854 + +0.6 44,855 - +0.2 44,855 - +0.0 44,855 - -0.8 44,855 - +0.0 45,241 - GFF4949 0.23 +0.4 45,483 + GFF4949 0.39 -0.6 45,483 + GFF4949 0.39 +0.1 45,484 - GFF4949 0.39 -0.1 45,484 - GFF4949 0.39 -0.5 45,484 - GFF4949 0.39 +0.4 45,509 - GFF4949 0.41 -0.5 45,630 + GFF4949 0.49 +1.5 45,630 + GFF4949 0.49 -1.3 45,630 + GFF4949 0.49 +1.3 45,630 + GFF4949 0.49 +0.1 45,630 + GFF4949 0.49 -0.1 45,631 - GFF4949 0.49 -3.3 45,631 - GFF4949 0.49 +0.2 45,631 - GFF4949 0.49 -0.1 45,631 - GFF4949 0.49 -0.0 45,631 - GFF4949 0.49 +0.9 45,631 - GFF4949 0.49 -0.1 45,634 - GFF4949 0.49 +1.2 45,634 - GFF4949 0.49 +0.3 45,867 + GFF4949 0.64 -0.2 45,867 + GFF4949 0.64 +0.1 45,868 - GFF4949 0.64 +0.0
Or see this region's nucleotide sequence