Strain Fitness in Variovorax sp. SCN45 around GFF4736

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4734 and GFF4735 are separated by 24 nucleotidesGFF4735 and GFF4736 overlap by 4 nucleotidesGFF4736 and GFF4737 are separated by 13 nucleotides GFF4734 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 98,751 to 101,219 GFF4734 GFF4735 - Nitrate ABC transporter, ATP-binding protein, at 101,244 to 102,053 GFF4735 GFF4736 - Nitrate ABC transporter, permease protein, at 102,050 to 102,970 GFF4736 GFF4737 - Nitrate ABC transporter, substrate-binding protein, at 102,984 to 104,228 GFF4737 Position (kb) 102 103Strain fitness (log2 ratio) -2 -1 0 1 2 3at 101.077 kb on + strandat 101.077 kb on + strandat 101.077 kb on + strandat 101.078 kb on - strandat 101.078 kb on - strandat 101.116 kb on + strandat 101.116 kb on + strandat 101.116 kb on + strandat 101.281 kb on + strandat 101.281 kb on + strandat 101.282 kb on - strandat 101.317 kb on + strandat 101.317 kb on + strandat 101.317 kb on + strandat 101.317 kb on + strandat 101.318 kb on - strandat 101.749 kb on + strand, within GFF4735at 101.749 kb on + strand, within GFF4735at 101.749 kb on + strand, within GFF4735at 101.749 kb on + strand, within GFF4735at 101.750 kb on - strand, within GFF4735at 101.750 kb on - strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.751 kb on + strand, within GFF4735at 101.752 kb on - strand, within GFF4735at 101.752 kb on - strand, within GFF4735at 101.752 kb on - strand, within GFF4735at 101.752 kb on - strand, within GFF4735at 102.034 kb on + strandat 102.034 kb on + strandat 102.034 kb on + strandat 102.035 kb on - strandat 102.282 kb on + strand, within GFF4736at 102.282 kb on + strand, within GFF4736at 102.282 kb on + strand, within GFF4736at 102.282 kb on + strand, within GFF4736at 102.282 kb on + strand, within GFF4736at 102.282 kb on + strand, within GFF4736at 102.282 kb on + strand, within GFF4736at 102.283 kb on - strand, within GFF4736at 102.360 kb on + strand, within GFF4736at 102.361 kb on - strand, within GFF4736at 102.361 kb on - strand, within GFF4736at 102.675 kb on + strand, within GFF4736at 102.675 kb on + strand, within GFF4736at 102.676 kb on - strand, within GFF4736at 102.676 kb on - strand, within GFF4736at 102.676 kb on - strand, within GFF4736at 102.676 kb on - strand, within GFF4736at 102.676 kb on - strand, within GFF4736at 103.012 kb on + strandat 103.012 kb on + strandat 103.117 kb on + strand, within GFF4737at 103.117 kb on + strand, within GFF4737at 103.117 kb on + strand, within GFF4737at 103.117 kb on + strand, within GFF4737at 103.117 kb on + strand, within GFF4737at 103.118 kb on - strand, within GFF4737at 103.118 kb on - strand, within GFF4737at 103.118 kb on - strand, within GFF4737at 103.156 kb on + strand, within GFF4737at 103.156 kb on + strand, within GFF4737at 103.157 kb on - strand, within GFF4737at 103.225 kb on + strand, within GFF4737at 103.225 kb on + strand, within GFF4737at 103.225 kb on + strand, within GFF4737at 103.226 kb on - strand, within GFF4737at 103.252 kb on + strand, within GFF4737at 103.252 kb on + strand, within GFF4737at 103.252 kb on + strand, within GFF4737at 103.253 kb on - strand, within GFF4737at 103.253 kb on - strand, within GFF4737at 103.303 kb on + strand, within GFF4737at 103.304 kb on - strand, within GFF4737at 103.351 kb on + strand, within GFF4737at 103.352 kb on - strand, within GFF4737at 103.352 kb on - strand, within GFF4737at 103.352 kb on - strand, within GFF4737at 103.352 kb on - strand, within GFF4737at 103.655 kb on - strand, within GFF4737at 103.702 kb on + strand, within GFF4737at 103.702 kb on + strand, within GFF4737at 103.750 kb on + strand, within GFF4737at 103.750 kb on + strand, within GFF4737at 103.750 kb on + strand, within GFF4737at 103.750 kb on + strand, within GFF4737at 103.751 kb on - strand, within GFF4737at 103.751 kb on - strand, within GFF4737at 103.913 kb on - strand, within GFF4737at 103.913 kb on - strand, within GFF4737at 103.924 kb on + strand, within GFF4737at 103.924 kb on + strand, within GFF4737at 103.925 kb on - strand, within GFF4737

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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101,077 + +0.3
101,077 + -0.2
101,077 + +0.4
101,078 - -0.7
101,078 - -0.6
101,116 + -0.7
101,116 + -0.3
101,116 + -0.6
101,281 + -0.2
101,281 + +0.9
101,282 - -0.3
101,317 + -2.0
101,317 + -0.0
101,317 + -0.8
101,317 + +0.0
101,318 - -0.1
101,749 + GFF4735 0.62 +0.6
101,749 + GFF4735 0.62 -1.2
101,749 + GFF4735 0.62 -1.2
101,749 + GFF4735 0.62 +0.1
101,750 - GFF4735 0.62 -0.9
101,750 - GFF4735 0.62 +0.1
101,751 + GFF4735 0.63 -0.7
101,751 + GFF4735 0.63 -0.1
101,751 + GFF4735 0.63 +0.0
101,751 + GFF4735 0.63 -0.3
101,751 + GFF4735 0.63 +0.6
101,751 + GFF4735 0.63 +2.9
101,751 + GFF4735 0.63 +0.3
101,751 + GFF4735 0.63 -0.3
101,752 - GFF4735 0.63 -0.3
101,752 - GFF4735 0.63 -0.4
101,752 - GFF4735 0.63 +0.4
101,752 - GFF4735 0.63 -0.4
102,034 + -0.7
102,034 + -0.4
102,034 + -0.4
102,035 - -0.7
102,282 + GFF4736 0.25 -0.1
102,282 + GFF4736 0.25 +1.1
102,282 + GFF4736 0.25 +0.1
102,282 + GFF4736 0.25 -2.7
102,282 + GFF4736 0.25 -1.8
102,282 + GFF4736 0.25 +1.3
102,282 + GFF4736 0.25 +1.3
102,283 - GFF4736 0.25 +1.9
102,360 + GFF4736 0.34 -0.4
102,361 - GFF4736 0.34 -0.0
102,361 - GFF4736 0.34 -1.3
102,675 + GFF4736 0.68 +0.3
102,675 + GFF4736 0.68 -0.0
102,676 - GFF4736 0.68 +0.7
102,676 - GFF4736 0.68 +0.5
102,676 - GFF4736 0.68 -0.4
102,676 - GFF4736 0.68 +0.2
102,676 - GFF4736 0.68 -0.4
103,012 + +0.0
103,012 + +0.4
103,117 + GFF4737 0.11 -0.3
103,117 + GFF4737 0.11 -0.4
103,117 + GFF4737 0.11 +0.3
103,117 + GFF4737 0.11 +1.1
103,117 + GFF4737 0.11 +0.0
103,118 - GFF4737 0.11 -2.0
103,118 - GFF4737 0.11 -0.4
103,118 - GFF4737 0.11 +0.5
103,156 + GFF4737 0.14 +0.4
103,156 + GFF4737 0.14 +2.0
103,157 - GFF4737 0.14 -0.2
103,225 + GFF4737 0.19 +0.5
103,225 + GFF4737 0.19 -0.2
103,225 + GFF4737 0.19 +0.8
103,226 - GFF4737 0.19 -0.4
103,252 + GFF4737 0.22 -0.6
103,252 + GFF4737 0.22 -0.3
103,252 + GFF4737 0.22 +0.3
103,253 - GFF4737 0.22 -1.1
103,253 - GFF4737 0.22 -0.1
103,303 + GFF4737 0.26 -0.7
103,304 - GFF4737 0.26 +0.9
103,351 + GFF4737 0.29 -0.5
103,352 - GFF4737 0.30 +0.5
103,352 - GFF4737 0.30 -0.2
103,352 - GFF4737 0.30 +0.2
103,352 - GFF4737 0.30 -0.6
103,655 - GFF4737 0.54 +0.7
103,702 + GFF4737 0.58 -0.0
103,702 + GFF4737 0.58 -0.5
103,750 + GFF4737 0.62 +0.9
103,750 + GFF4737 0.62 -0.2
103,750 + GFF4737 0.62 -2.3
103,750 + GFF4737 0.62 +0.7
103,751 - GFF4737 0.62 -0.1
103,751 - GFF4737 0.62 +0.1
103,913 - GFF4737 0.75 -0.5
103,913 - GFF4737 0.75 -1.0
103,924 + GFF4737 0.76 -0.4
103,924 + GFF4737 0.76 -0.0
103,925 - GFF4737 0.76 +0.6

Or see this region's nucleotide sequence