Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4624 and GFF4625 are separated by 107 nucleotides GFF4625 and GFF4626 are separated by 45 nucleotides GFF4626 and GFF4627 are separated by 0 nucleotides
GFF4624 - N-acetylglutamate kinase (EC 2.7.2.8), at 265,895 to 266,788
GFF4624
GFF4625 - Undecaprenyl-phosphate alpha-N-acetylglucosaminyl 1-phosphate transferase (EC 2.7.8.33), at 266,896 to 267,993
GFF4625
GFF4626 - dTDP-Rha:A-D-GlcNAc-diphosphoryl polyprenol, A-3-L-rhamnosyl transferase WbbL, at 268,039 to 268,800
GFF4626
GFF4627 - Glycosyltransferase, at 268,801 to 269,784
GFF4627
Position (kb) 266
267
268 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 266.942 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.954 kb on + strand at 266.955 kb on - strand at 266.955 kb on - strand at 266.955 kb on - strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.993 kb on + strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 266.994 kb on - strand at 267.053 kb on + strand, within GFF4625 at 267.054 kb on - strand, within GFF4625 at 267.054 kb on - strand, within GFF4625 at 267.197 kb on + strand, within GFF4625 at 267.198 kb on - strand, within GFF4625 at 267.198 kb on - strand, within GFF4625 at 267.198 kb on - strand, within GFF4625 at 267.228 kb on - strand, within GFF4625 at 267.320 kb on + strand, within GFF4625 at 267.320 kb on + strand, within GFF4625 at 267.321 kb on - strand, within GFF4625 at 267.321 kb on - strand, within GFF4625 at 267.359 kb on + strand, within GFF4625 at 267.360 kb on - strand, within GFF4625 at 267.497 kb on + strand, within GFF4625 at 267.905 kb on + strand at 267.906 kb on - strand at 268.014 kb on + strand at 268.015 kb on - strand at 268.015 kb on - strand at 268.015 kb on - strand at 268.015 kb on - strand at 268.015 kb on - strand at 268.015 kb on - strand at 268.034 kb on - strand at 268.486 kb on + strand, within GFF4626 at 268.486 kb on + strand, within GFF4626 at 268.487 kb on - strand, within GFF4626 at 268.588 kb on + strand, within GFF4626 at 268.589 kb on - strand, within GFF4626 at 268.589 kb on - strand, within GFF4626 at 268.705 kb on + strand, within GFF4626 at 268.705 kb on + strand, within GFF4626 at 268.706 kb on - strand, within GFF4626 at 268.706 kb on - strand, within GFF4626 at 268.711 kb on + strand, within GFF4626 at 268.711 kb on + strand, within GFF4626 at 268.711 kb on + strand, within GFF4626 at 268.712 kb on - strand, within GFF4626 at 268.712 kb on - strand, within GFF4626 at 268.712 kb on - strand, within GFF4626
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 266,942 + -2.6 266,954 + -2.6 266,954 + -4.0 266,954 + -0.9 266,954 + -2.1 266,954 + -2.5 266,954 + -2.2 266,954 + -1.9 266,954 + -4.0 266,955 - -1.7 266,955 - -1.8 266,955 - -0.9 266,993 + -0.7 266,993 + -0.2 266,993 + -2.2 266,993 + -1.9 266,993 + -2.9 266,993 + -1.0 266,993 + -3.5 266,993 + -1.7 266,994 - -0.7 266,994 - -3.8 266,994 - -1.5 266,994 - -2.3 266,994 - -4.5 266,994 - -1.8 266,994 - -2.2 266,994 - -1.9 267,053 + GFF4625 0.14 -0.9 267,054 - GFF4625 0.14 -2.9 267,054 - GFF4625 0.14 -1.7 267,197 + GFF4625 0.27 -2.4 267,198 - GFF4625 0.28 -2.0 267,198 - GFF4625 0.28 -3.1 267,198 - GFF4625 0.28 -0.7 267,228 - GFF4625 0.30 -0.9 267,320 + GFF4625 0.39 -1.5 267,320 + GFF4625 0.39 -2.1 267,321 - GFF4625 0.39 -1.3 267,321 - GFF4625 0.39 -1.1 267,359 + GFF4625 0.42 -4.3 267,360 - GFF4625 0.42 -3.4 267,497 + GFF4625 0.55 -3.3 267,905 + +1.1 267,906 - -2.5 268,014 + -0.3 268,015 - -0.9 268,015 - +0.2 268,015 - -0.9 268,015 - +1.0 268,015 - -0.5 268,015 - +0.0 268,034 - -0.5 268,486 + GFF4626 0.59 +0.9 268,486 + GFF4626 0.59 -1.5 268,487 - GFF4626 0.59 -1.3 268,588 + GFF4626 0.72 -3.4 268,589 - GFF4626 0.72 -3.3 268,589 - GFF4626 0.72 -3.0 268,705 + GFF4626 0.87 -0.9 268,705 + GFF4626 0.87 -0.3 268,706 - GFF4626 0.88 -1.6 268,706 - GFF4626 0.88 -2.6 268,711 + GFF4626 0.88 -0.8 268,711 + GFF4626 0.88 -2.5 268,711 + GFF4626 0.88 -2.2 268,712 - GFF4626 0.88 -0.7 268,712 - GFF4626 0.88 -3.0 268,712 - GFF4626 0.88 -4.2
Or see this region's nucleotide sequence