Strain Fitness in Variovorax sp. SCN45 around GFF461

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF460 and GFF461 are separated by 15 nucleotidesGFF461 and GFF462 overlap by 4 nucleotides GFF460 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 498,601 to 500,139 GFF460 GFF461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 500,155 to 500,955 GFF461 GFF462 - BUG/TctC family periplasmic protein, at 500,952 to 501,938 GFF462 Position (kb) 500 501Strain fitness (log2 ratio) -2 -1 0 1 2 3at 499.184 kb on + strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.184 kb on + strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.185 kb on - strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.364 kb on + strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.365 kb on - strand, within GFF460at 499.553 kb on + strand, within GFF460at 499.553 kb on + strand, within GFF460at 499.554 kb on - strand, within GFF460at 499.554 kb on - strand, within GFF460at 499.637 kb on + strand, within GFF460at 499.638 kb on - strand, within GFF460at 499.806 kb on + strand, within GFF460at 499.806 kb on + strand, within GFF460at 499.806 kb on + strand, within GFF460at 499.807 kb on - strand, within GFF460at 499.807 kb on - strand, within GFF460at 499.841 kb on + strand, within GFF460at 499.845 kb on - strand, within GFF460at 499.892 kb on + strand, within GFF460at 499.916 kb on + strand, within GFF460at 499.916 kb on + strand, within GFF460at 499.916 kb on + strand, within GFF460at 499.917 kb on - strand, within GFF460at 499.917 kb on - strand, within GFF460at 500.039 kb on + strandat 500.040 kb on - strandat 500.148 kb on + strandat 500.148 kb on + strandat 500.148 kb on + strandat 500.149 kb on - strandat 500.149 kb on - strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.151 kb on + strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.152 kb on - strandat 500.222 kb on + strandat 500.222 kb on + strandat 500.222 kb on + strandat 500.222 kb on + strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.223 kb on - strandat 500.228 kb on + strandat 500.228 kb on + strandat 500.229 kb on - strandat 500.402 kb on + strand, within GFF461at 500.402 kb on + strand, within GFF461at 500.523 kb on + strand, within GFF461at 500.524 kb on - strand, within GFF461at 501.170 kb on - strand, within GFF462at 501.187 kb on + strand, within GFF462at 501.187 kb on + strand, within GFF462at 501.188 kb on - strand, within GFF462at 501.188 kb on - strand, within GFF462at 501.188 kb on - strand, within GFF462at 501.219 kb on + strand, within GFF462at 501.219 kb on + strand, within GFF462at 501.219 kb on + strand, within GFF462at 501.219 kb on + strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.220 kb on - strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.289 kb on + strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.290 kb on - strand, within GFF462at 501.460 kb on + strand, within GFF462at 501.460 kb on + strand, within GFF462at 501.601 kb on + strand, within GFF462at 501.665 kb on - strand, within GFF462

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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499,184 + GFF460 0.38 -0.1
499,184 + GFF460 0.38 +0.2
499,184 + GFF460 0.38 -0.1
499,185 - GFF460 0.38 +0.2
499,185 - GFF460 0.38 -0.7
499,185 - GFF460 0.38 +0.3
499,185 - GFF460 0.38 -0.8
499,185 - GFF460 0.38 -0.1
499,364 + GFF460 0.50 +0.6
499,364 + GFF460 0.50 +0.3
499,364 + GFF460 0.50 -0.5
499,364 + GFF460 0.50 -1.5
499,365 - GFF460 0.50 -0.7
499,365 - GFF460 0.50 -0.1
499,365 - GFF460 0.50 -0.3
499,365 - GFF460 0.50 +0.0
499,365 - GFF460 0.50 -0.8
499,553 + GFF460 0.62 -0.4
499,553 + GFF460 0.62 +0.1
499,554 - GFF460 0.62 +0.2
499,554 - GFF460 0.62 -0.5
499,637 + GFF460 0.67 +0.9
499,638 - GFF460 0.67 -0.1
499,806 + GFF460 0.78 +0.5
499,806 + GFF460 0.78 +1.0
499,806 + GFF460 0.78 -1.0
499,807 - GFF460 0.78 -0.8
499,807 - GFF460 0.78 +0.2
499,841 + GFF460 0.81 -0.3
499,845 - GFF460 0.81 -0.8
499,892 + GFF460 0.84 +0.6
499,916 + GFF460 0.85 -0.1
499,916 + GFF460 0.85 +0.2
499,916 + GFF460 0.85 -0.4
499,917 - GFF460 0.86 +1.2
499,917 - GFF460 0.86 -0.6
500,039 + +0.2
500,040 - +1.2
500,148 + -0.0
500,148 + +0.2
500,148 + -1.6
500,149 - -0.7
500,149 - +0.7
500,151 + +0.8
500,151 + -0.4
500,151 + +0.5
500,151 + -0.4
500,151 + -0.4
500,151 + -0.6
500,152 - +0.2
500,152 - -1.6
500,152 - -0.0
500,152 - -1.0
500,152 - +2.2
500,222 + -0.6
500,222 + -0.2
500,222 + -0.4
500,222 + +0.4
500,223 - +0.7
500,223 - +0.5
500,223 - -0.1
500,223 - +0.2
500,223 - +0.4
500,223 - +0.0
500,228 + +0.1
500,228 + -0.5
500,229 - +1.2
500,402 + GFF461 0.31 +0.6
500,402 + GFF461 0.31 -0.3
500,523 + GFF461 0.46 -0.7
500,524 - GFF461 0.46 -1.1
501,170 - GFF462 0.22 +0.1
501,187 + GFF462 0.24 -0.2
501,187 + GFF462 0.24 +2.8
501,188 - GFF462 0.24 -0.2
501,188 - GFF462 0.24 -0.2
501,188 - GFF462 0.24 -0.9
501,219 + GFF462 0.27 -0.4
501,219 + GFF462 0.27 -1.4
501,219 + GFF462 0.27 -1.1
501,219 + GFF462 0.27 +0.3
501,220 - GFF462 0.27 -1.7
501,220 - GFF462 0.27 -1.0
501,220 - GFF462 0.27 -0.9
501,220 - GFF462 0.27 -0.3
501,220 - GFF462 0.27 -0.7
501,220 - GFF462 0.27 -0.3
501,220 - GFF462 0.27 -1.3
501,220 - GFF462 0.27 +1.2
501,289 + GFF462 0.34 -1.3
501,289 + GFF462 0.34 -0.3
501,289 + GFF462 0.34 +0.3
501,289 + GFF462 0.34 -0.1
501,289 + GFF462 0.34 +0.3
501,289 + GFF462 0.34 -1.4
501,289 + GFF462 0.34 +0.6
501,289 + GFF462 0.34 +0.4
501,290 - GFF462 0.34 +0.4
501,290 - GFF462 0.34 -0.6
501,290 - GFF462 0.34 +0.8
501,290 - GFF462 0.34 +0.5
501,290 - GFF462 0.34 -1.1
501,290 - GFF462 0.34 -0.3
501,290 - GFF462 0.34 +0.2
501,460 + GFF462 0.51 +0.0
501,460 + GFF462 0.51 -1.8
501,601 + GFF462 0.66 -0.8
501,665 - GFF462 0.72 -0.3

Or see this region's nucleotide sequence