Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4529 and GFF4530 are separated by 170 nucleotides GFF4530 and GFF4531 overlap by 4 nucleotides GFF4531 and GFF4532 are separated by 140 nucleotides
GFF4529 - Probable signal peptide protein, at 167,148 to 167,978
GFF4529
GFF4530 - MBL-fold metallo-hydrolase superfamily, at 168,149 to 169,825
GFF4530
GFF4531 - Pseudouridine synthase, at 169,822 to 170,547
GFF4531
GFF4532 - hypothetical protein, at 170,688 to 171,233
GFF4532
Position (kb)
168
169
170 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 167.259 kb on + strand, within GFF4529 at 167.259 kb on + strand, within GFF4529 at 167.260 kb on - strand, within GFF4529 at 167.260 kb on - strand, within GFF4529 at 167.260 kb on - strand, within GFF4529 at 167.260 kb on - strand, within GFF4529 at 167.336 kb on - strand, within GFF4529 at 167.336 kb on - strand, within GFF4529 at 167.336 kb on - strand, within GFF4529 at 167.336 kb on - strand, within GFF4529 at 167.336 kb on - strand, within GFF4529 at 167.353 kb on - strand, within GFF4529 at 167.376 kb on + strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.377 kb on - strand, within GFF4529 at 167.439 kb on + strand, within GFF4529 at 167.439 kb on + strand, within GFF4529 at 167.439 kb on + strand, within GFF4529 at 167.439 kb on + strand, within GFF4529 at 167.439 kb on + strand, within GFF4529 at 167.440 kb on - strand, within GFF4529 at 167.440 kb on - strand, within GFF4529 at 167.440 kb on - strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.496 kb on + strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.497 kb on - strand, within GFF4529 at 167.502 kb on + strand, within GFF4529 at 167.502 kb on + strand, within GFF4529 at 167.503 kb on - strand, within GFF4529 at 167.503 kb on - strand, within GFF4529 at 167.503 kb on - strand, within GFF4529 at 167.526 kb on - strand, within GFF4529 at 167.526 kb on - strand, within GFF4529 at 167.538 kb on + strand, within GFF4529 at 167.553 kb on + strand, within GFF4529 at 167.589 kb on + strand, within GFF4529 at 167.613 kb on + strand, within GFF4529 at 167.614 kb on - strand, within GFF4529 at 167.614 kb on - strand, within GFF4529 at 167.614 kb on - strand, within GFF4529 at 167.685 kb on + strand, within GFF4529 at 167.686 kb on - strand, within GFF4529 at 167.686 kb on - strand, within GFF4529 at 167.686 kb on - strand, within GFF4529 at 167.686 kb on - strand, within GFF4529 at 167.734 kb on - strand, within GFF4529 at 167.734 kb on - strand, within GFF4529 at 167.769 kb on + strand, within GFF4529 at 167.770 kb on - strand, within GFF4529 at 167.788 kb on - strand, within GFF4529 at 167.805 kb on + strand, within GFF4529 at 167.805 kb on + strand, within GFF4529 at 167.806 kb on - strand, within GFF4529 at 167.806 kb on - strand, within GFF4529 at 167.806 kb on - strand, within GFF4529 at 167.868 kb on + strand, within GFF4529 at 167.868 kb on + strand, within GFF4529 at 167.868 kb on + strand, within GFF4529 at 167.869 kb on - strand, within GFF4529 at 167.869 kb on - strand, within GFF4529 at 167.869 kb on - strand, within GFF4529 at 167.928 kb on + strand at 167.928 kb on + strand at 167.928 kb on + strand at 167.929 kb on - strand at 167.929 kb on - strand at 167.929 kb on - strand at 167.929 kb on - strand at 167.929 kb on - strand at 167.951 kb on + strand at 167.976 kb on + strand at 167.976 kb on + strand at 167.977 kb on - strand at 167.977 kb on - strand at 167.977 kb on - strand at 168.038 kb on + strand at 168.038 kb on + strand at 168.039 kb on - strand at 168.299 kb on + strand at 168.299 kb on + strand at 168.299 kb on + strand at 168.300 kb on - strand at 168.300 kb on - strand at 168.300 kb on - strand at 168.300 kb on - strand at 168.300 kb on - strand at 168.483 kb on + strand, within GFF4530 at 168.483 kb on + strand, within GFF4530 at 168.483 kb on + strand, within GFF4530 at 168.483 kb on + strand, within GFF4530 at 168.483 kb on + strand, within GFF4530 at 168.484 kb on - strand, within GFF4530 at 168.792 kb on - strand, within GFF4530 at 168.902 kb on + strand, within GFF4530 at 168.902 kb on + strand, within GFF4530 at 168.903 kb on - strand, within GFF4530 at 168.903 kb on - strand, within GFF4530 at 168.903 kb on - strand, within GFF4530 at 168.903 kb on - strand, within GFF4530 at 168.903 kb on - strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.904 kb on + strand, within GFF4530 at 168.905 kb on - strand, within GFF4530 at 168.905 kb on - strand, within GFF4530 at 168.905 kb on - strand, within GFF4530 at 168.905 kb on - strand, within GFF4530 at 169.070 kb on + strand, within GFF4530 at 169.070 kb on + strand, within GFF4530 at 169.070 kb on + strand, within GFF4530 at 169.070 kb on + strand, within GFF4530 at 169.070 kb on + strand, within GFF4530 at 169.070 kb on + strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.071 kb on - strand, within GFF4530 at 169.098 kb on - strand, within GFF4530 at 169.529 kb on + strand, within GFF4530 at 169.530 kb on - strand, within GFF4530 at 169.530 kb on - strand, within GFF4530 at 169.530 kb on - strand, within GFF4530 at 169.530 kb on - strand, within GFF4530 at 169.604 kb on + strand, within GFF4530 at 169.604 kb on + strand, within GFF4530 at 169.733 kb on + strand at 169.827 kb on + strand at 169.827 kb on + strand at 169.828 kb on - strand at 169.828 kb on - strand at 169.828 kb on - strand at 169.925 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.056 kb on + strand, within GFF4531 at 170.057 kb on - strand, within GFF4531 at 170.216 kb on - strand, within GFF4531 at 170.216 kb on - strand, within GFF4531 at 170.521 kb on + strand at 170.522 kb on - strand at 170.522 kb on - strand at 170.522 kb on - strand at 170.522 kb on - strand at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.757 kb on + strand, within GFF4532 at 170.758 kb on - strand, within GFF4532 at 170.758 kb on - strand, within GFF4532
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 167,259 + GFF4529 0.13 +0.0 167,259 + GFF4529 0.13 +0.2 167,260 - GFF4529 0.13 -0.5 167,260 - GFF4529 0.13 -0.3 167,260 - GFF4529 0.13 -0.7 167,260 - GFF4529 0.13 -0.1 167,336 - GFF4529 0.23 -0.4 167,336 - GFF4529 0.23 +0.4 167,336 - GFF4529 0.23 -0.5 167,336 - GFF4529 0.23 -0.5 167,336 - GFF4529 0.23 -0.2 167,353 - GFF4529 0.25 +0.1 167,376 + GFF4529 0.27 +0.7 167,377 - GFF4529 0.28 +0.7 167,377 - GFF4529 0.28 -0.2 167,377 - GFF4529 0.28 +0.3 167,377 - GFF4529 0.28 +0.2 167,377 - GFF4529 0.28 +0.4 167,377 - GFF4529 0.28 -0.5 167,377 - GFF4529 0.28 -0.7 167,377 - GFF4529 0.28 +0.6 167,439 + GFF4529 0.35 -0.1 167,439 + GFF4529 0.35 -0.1 167,439 + GFF4529 0.35 +0.0 167,439 + GFF4529 0.35 -0.7 167,439 + GFF4529 0.35 -0.6 167,440 - GFF4529 0.35 -0.3 167,440 - GFF4529 0.35 -0.8 167,440 - GFF4529 0.35 +0.2 167,496 + GFF4529 0.42 +0.2 167,496 + GFF4529 0.42 -0.5 167,496 + GFF4529 0.42 +1.4 167,496 + GFF4529 0.42 -0.2 167,496 + GFF4529 0.42 +0.2 167,496 + GFF4529 0.42 +0.1 167,496 + GFF4529 0.42 +1.0 167,496 + GFF4529 0.42 +0.7 167,496 + GFF4529 0.42 -0.5 167,496 + GFF4529 0.42 -0.2 167,496 + GFF4529 0.42 -0.5 167,496 + GFF4529 0.42 +1.1 167,496 + GFF4529 0.42 +0.6 167,496 + GFF4529 0.42 -0.0 167,496 + GFF4529 0.42 -1.1 167,497 - GFF4529 0.42 -0.0 167,497 - GFF4529 0.42 +0.3 167,497 - GFF4529 0.42 +0.1 167,497 - GFF4529 0.42 +0.4 167,497 - GFF4529 0.42 -0.3 167,497 - GFF4529 0.42 +0.7 167,497 - GFF4529 0.42 -0.8 167,497 - GFF4529 0.42 -0.3 167,497 - GFF4529 0.42 -0.6 167,497 - GFF4529 0.42 +0.9 167,497 - GFF4529 0.42 +0.1 167,497 - GFF4529 0.42 -0.8 167,497 - GFF4529 0.42 +0.1 167,497 - GFF4529 0.42 +0.5 167,502 + GFF4529 0.43 +1.2 167,502 + GFF4529 0.43 -0.7 167,503 - GFF4529 0.43 -0.2 167,503 - GFF4529 0.43 +0.1 167,503 - GFF4529 0.43 -0.4 167,526 - GFF4529 0.45 -0.9 167,526 - GFF4529 0.45 +0.2 167,538 + GFF4529 0.47 +0.1 167,553 + GFF4529 0.49 -0.4 167,589 + GFF4529 0.53 -0.3 167,613 + GFF4529 0.56 +1.1 167,614 - GFF4529 0.56 -1.8 167,614 - GFF4529 0.56 -0.4 167,614 - GFF4529 0.56 +0.5 167,685 + GFF4529 0.65 -0.2 167,686 - GFF4529 0.65 -0.1 167,686 - GFF4529 0.65 +0.2 167,686 - GFF4529 0.65 +0.4 167,686 - GFF4529 0.65 +0.0 167,734 - GFF4529 0.71 -0.5 167,734 - GFF4529 0.71 -0.3 167,769 + GFF4529 0.75 +0.2 167,770 - GFF4529 0.75 -0.4 167,788 - GFF4529 0.77 -0.2 167,805 + GFF4529 0.79 -0.4 167,805 + GFF4529 0.79 -0.0 167,806 - GFF4529 0.79 -0.9 167,806 - GFF4529 0.79 -0.7 167,806 - GFF4529 0.79 -1.0 167,868 + GFF4529 0.87 -0.3 167,868 + GFF4529 0.87 +0.3 167,868 + GFF4529 0.87 +0.5 167,869 - GFF4529 0.87 -0.8 167,869 - GFF4529 0.87 -0.6 167,869 - GFF4529 0.87 -1.2 167,928 + +0.9 167,928 + -0.5 167,928 + +0.5 167,929 - +0.0 167,929 - -0.4 167,929 - +0.6 167,929 - +0.4 167,929 - -0.2 167,951 + -0.5 167,976 + -0.4 167,976 + -1.3 167,977 - +0.1 167,977 - -0.4 167,977 - -0.4 168,038 + +0.5 168,038 + +0.1 168,039 - +0.1 168,299 + -1.5 168,299 + -0.7 168,299 + -0.9 168,300 - -0.7 168,300 - -0.3 168,300 - -1.9 168,300 - -0.2 168,300 - +0.2 168,483 + GFF4530 0.20 -1.6 168,483 + GFF4530 0.20 +0.4 168,483 + GFF4530 0.20 -2.9 168,483 + GFF4530 0.20 -1.0 168,483 + GFF4530 0.20 +0.1 168,484 - GFF4530 0.20 -2.1 168,792 - GFF4530 0.38 -1.1 168,902 + GFF4530 0.45 -1.5 168,902 + GFF4530 0.45 -0.4 168,903 - GFF4530 0.45 -1.6 168,903 - GFF4530 0.45 -1.1 168,903 - GFF4530 0.45 -2.1 168,903 - GFF4530 0.45 -3.2 168,903 - GFF4530 0.45 -2.7 168,904 + GFF4530 0.45 -1.5 168,904 + GFF4530 0.45 -1.7 168,904 + GFF4530 0.45 +0.5 168,904 + GFF4530 0.45 +0.2 168,904 + GFF4530 0.45 -2.5 168,904 + GFF4530 0.45 -0.7 168,904 + GFF4530 0.45 -1.3 168,904 + GFF4530 0.45 +0.4 168,904 + GFF4530 0.45 -2.4 168,904 + GFF4530 0.45 -1.9 168,905 - GFF4530 0.45 +1.1 168,905 - GFF4530 0.45 -0.7 168,905 - GFF4530 0.45 -0.7 168,905 - GFF4530 0.45 -1.5 169,070 + GFF4530 0.55 -0.5 169,070 + GFF4530 0.55 -0.9 169,070 + GFF4530 0.55 +1.7 169,070 + GFF4530 0.55 -0.1 169,070 + GFF4530 0.55 -2.7 169,070 + GFF4530 0.55 -2.0 169,071 - GFF4530 0.55 +0.5 169,071 - GFF4530 0.55 -1.4 169,071 - GFF4530 0.55 -1.0 169,071 - GFF4530 0.55 +0.7 169,071 - GFF4530 0.55 -2.0 169,071 - GFF4530 0.55 -0.7 169,071 - GFF4530 0.55 -1.8 169,071 - GFF4530 0.55 -1.5 169,071 - GFF4530 0.55 -1.5 169,098 - GFF4530 0.57 -1.5 169,529 + GFF4530 0.82 -2.1 169,530 - GFF4530 0.82 -1.3 169,530 - GFF4530 0.82 -1.2 169,530 - GFF4530 0.82 +1.1 169,530 - GFF4530 0.82 -2.2 169,604 + GFF4530 0.87 -1.4 169,604 + GFF4530 0.87 -1.1 169,733 + -2.7 169,827 + -0.1 169,827 + -0.0 169,828 - +0.4 169,828 - -0.7 169,828 - -0.3 169,925 + GFF4531 0.14 +0.2 170,056 + GFF4531 0.32 +0.4 170,056 + GFF4531 0.32 -0.4 170,056 + GFF4531 0.32 -0.7 170,056 + GFF4531 0.32 -0.1 170,056 + GFF4531 0.32 -0.6 170,056 + GFF4531 0.32 -0.8 170,057 - GFF4531 0.32 -1.7 170,216 - GFF4531 0.54 -0.7 170,216 - GFF4531 0.54 +0.2 170,521 + -0.7 170,522 - -0.5 170,522 - -0.1 170,522 - +0.6 170,522 - +1.7 170,757 + GFF4532 0.13 +0.3 170,757 + GFF4532 0.13 +0.1 170,757 + GFF4532 0.13 +0.5 170,757 + GFF4532 0.13 +0.2 170,757 + GFF4532 0.13 +0.7 170,757 + GFF4532 0.13 -1.0 170,758 - GFF4532 0.13 +0.0 170,758 - GFF4532 0.13 -0.1
Or see this region's nucleotide sequence