Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4517 and GFF4518 are separated by 57 nucleotides GFF4518 and GFF4519 are separated by 22 nucleotides GFF4519 and GFF4520 are separated by 39 nucleotides GFF4520 and GFF4521 are separated by 28 nucleotides
GFF4517 - Uncharacterized MFS-type transporter YtbD, at 157,481 to 158,683
GFF4517
GFF4518 - Methylglyoxal reductase, dihydroxyacetone producing @ 2,5-didehydrogluconate reductase (2-dehydro-L-gulonate-forming) (EC 1.1.1.346), at 158,741 to 159,565
GFF4518
GFF4519 - no description, at 159,588 to 159,929
GFF4519
GFF4520 - hypothetical protein, at 159,969 to 160,391
GFF4520
GFF4521 - Guanine deaminase (EC 3.5.4.3), at 160,420 to 160,914
GFF4521
Position (kb) 158
159
160 Strain fitness (log2 ratio) -2
-1
0
1
2 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.751 kb on + strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 157.752 kb on - strand, within GFF4517 at 158.096 kb on + strand, within GFF4517 at 158.096 kb on + strand, within GFF4517 at 158.096 kb on + strand, within GFF4517 at 158.096 kb on + strand, within GFF4517 at 158.096 kb on + strand, within GFF4517 at 158.097 kb on - strand, within GFF4517 at 158.097 kb on - strand, within GFF4517 at 158.097 kb on - strand, within GFF4517 at 158.097 kb on - strand, within GFF4517 at 158.118 kb on - strand, within GFF4517 at 158.213 kb on + strand, within GFF4517 at 158.213 kb on + strand, within GFF4517 at 158.213 kb on + strand, within GFF4517 at 158.213 kb on + strand, within GFF4517 at 158.214 kb on - strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.420 kb on + strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.421 kb on - strand, within GFF4517 at 158.876 kb on + strand, within GFF4518 at 158.876 kb on + strand, within GFF4518 at 158.876 kb on + strand, within GFF4518 at 158.877 kb on - strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.969 kb on + strand, within GFF4518 at 158.970 kb on - strand, within GFF4518 at 158.970 kb on - strand, within GFF4518 at 158.970 kb on - strand, within GFF4518 at 158.970 kb on - strand, within GFF4518 at 159.156 kb on - strand, within GFF4518 at 159.165 kb on + strand, within GFF4518 at 159.233 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.293 kb on + strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.294 kb on - strand, within GFF4518 at 159.308 kb on + strand, within GFF4518 at 159.408 kb on - strand, within GFF4518 at 159.569 kb on + strand at 159.569 kb on + strand at 159.570 kb on - strand at 159.570 kb on - strand at 159.570 kb on - strand at 159.712 kb on + strand, within GFF4519 at 159.712 kb on + strand, within GFF4519 at 159.712 kb on + strand, within GFF4519 at 159.712 kb on + strand, within GFF4519 at 159.740 kb on - strand, within GFF4519 at 159.740 kb on - strand, within GFF4519 at 159.740 kb on - strand, within GFF4519 at 159.740 kb on - strand, within GFF4519 at 159.740 kb on - strand, within GFF4519 at 159.794 kb on - strand, within GFF4519 at 159.794 kb on - strand, within GFF4519 at 159.794 kb on - strand, within GFF4519 at 159.794 kb on - strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.853 kb on + strand, within GFF4519 at 159.854 kb on - strand, within GFF4519 at 159.854 kb on - strand, within GFF4519 at 159.854 kb on - strand, within GFF4519 at 159.854 kb on - strand, within GFF4519 at 159.857 kb on + strand, within GFF4519 at 159.857 kb on + strand, within GFF4519 at 159.857 kb on + strand, within GFF4519 at 159.857 kb on + strand, within GFF4519 at 159.857 kb on + strand, within GFF4519 at 159.858 kb on - strand, within GFF4519 at 159.858 kb on - strand, within GFF4519 at 159.858 kb on - strand, within GFF4519 at 159.874 kb on - strand, within GFF4519 at 159.874 kb on - strand, within GFF4519 at 159.922 kb on + strand at 159.936 kb on + strand at 159.937 kb on - strand at 160.114 kb on + strand, within GFF4520 at 160.114 kb on + strand, within GFF4520 at 160.114 kb on + strand, within GFF4520 at 160.114 kb on + strand, within GFF4520 at 160.115 kb on - strand, within GFF4520 at 160.115 kb on - strand, within GFF4520 at 160.132 kb on + strand, within GFF4520 at 160.132 kb on + strand, within GFF4520 at 160.132 kb on + strand, within GFF4520 at 160.132 kb on + strand, within GFF4520 at 160.133 kb on - strand, within GFF4520 at 160.133 kb on - strand, within GFF4520 at 160.133 kb on - strand, within GFF4520 at 160.133 kb on - strand, within GFF4520 at 160.133 kb on - strand, within GFF4520 at 160.213 kb on + strand, within GFF4520 at 160.213 kb on + strand, within GFF4520 at 160.214 kb on - strand, within GFF4520 at 160.214 kb on - strand, within GFF4520 at 160.214 kb on - strand, within GFF4520 at 160.228 kb on - strand, within GFF4520 at 160.262 kb on - strand, within GFF4520 at 160.268 kb on - strand, within GFF4520 at 160.268 kb on - strand, within GFF4520 at 160.348 kb on + strand, within GFF4520 at 160.348 kb on + strand, within GFF4520 at 160.348 kb on + strand, within GFF4520 at 160.348 kb on + strand, within GFF4520 at 160.349 kb on - strand at 160.349 kb on - strand at 160.349 kb on - strand at 160.386 kb on - strand at 160.386 kb on - strand at 160.386 kb on - strand at 160.386 kb on - strand at 160.386 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 157,751 + GFF4517 0.22 +1.2 157,751 + GFF4517 0.22 +0.1 157,751 + GFF4517 0.22 -0.4 157,751 + GFF4517 0.22 -0.1 157,751 + GFF4517 0.22 -0.0 157,751 + GFF4517 0.22 +0.4 157,751 + GFF4517 0.22 -0.3 157,751 + GFF4517 0.22 -2.1 157,751 + GFF4517 0.22 +1.4 157,751 + GFF4517 0.22 -0.2 157,751 + GFF4517 0.22 +0.0 157,752 - GFF4517 0.23 -0.2 157,752 - GFF4517 0.23 +1.0 157,752 - GFF4517 0.23 -1.5 157,752 - GFF4517 0.23 -0.1 157,752 - GFF4517 0.23 +2.2 157,752 - GFF4517 0.23 -0.1 157,752 - GFF4517 0.23 -0.2 157,752 - GFF4517 0.23 -0.5 157,752 - GFF4517 0.23 -0.6 158,096 + GFF4517 0.51 -0.4 158,096 + GFF4517 0.51 -0.0 158,096 + GFF4517 0.51 -0.5 158,096 + GFF4517 0.51 +0.1 158,096 + GFF4517 0.51 +0.2 158,097 - GFF4517 0.51 -0.3 158,097 - GFF4517 0.51 -1.2 158,097 - GFF4517 0.51 +0.8 158,097 - GFF4517 0.51 -1.0 158,118 - GFF4517 0.53 +0.0 158,213 + GFF4517 0.61 +0.7 158,213 + GFF4517 0.61 -0.5 158,213 + GFF4517 0.61 +0.0 158,213 + GFF4517 0.61 +1.7 158,214 - GFF4517 0.61 -0.1 158,420 + GFF4517 0.78 -1.3 158,420 + GFF4517 0.78 +0.7 158,420 + GFF4517 0.78 +1.0 158,420 + GFF4517 0.78 -1.3 158,420 + GFF4517 0.78 -0.8 158,420 + GFF4517 0.78 -0.1 158,420 + GFF4517 0.78 -0.8 158,420 + GFF4517 0.78 -0.2 158,420 + GFF4517 0.78 -1.1 158,420 + GFF4517 0.78 +0.5 158,420 + GFF4517 0.78 -1.0 158,420 + GFF4517 0.78 +0.4 158,420 + GFF4517 0.78 -1.1 158,420 + GFF4517 0.78 -1.1 158,420 + GFF4517 0.78 -0.7 158,420 + GFF4517 0.78 +1.2 158,420 + GFF4517 0.78 -0.5 158,420 + GFF4517 0.78 +0.7 158,420 + GFF4517 0.78 -0.3 158,420 + GFF4517 0.78 -0.4 158,421 - GFF4517 0.78 -1.2 158,421 - GFF4517 0.78 +0.2 158,421 - GFF4517 0.78 -0.0 158,421 - GFF4517 0.78 +0.3 158,421 - GFF4517 0.78 -0.1 158,421 - GFF4517 0.78 +0.1 158,421 - GFF4517 0.78 +0.1 158,421 - GFF4517 0.78 -0.3 158,421 - GFF4517 0.78 +0.9 158,421 - GFF4517 0.78 +0.0 158,421 - GFF4517 0.78 +0.2 158,421 - GFF4517 0.78 +1.4 158,421 - GFF4517 0.78 +0.2 158,421 - GFF4517 0.78 -1.8 158,421 - GFF4517 0.78 -1.0 158,421 - GFF4517 0.78 -0.6 158,876 + GFF4518 0.16 -1.5 158,876 + GFF4518 0.16 -0.2 158,876 + GFF4518 0.16 -0.5 158,877 - GFF4518 0.16 +0.4 158,969 + GFF4518 0.28 +0.7 158,969 + GFF4518 0.28 -0.9 158,969 + GFF4518 0.28 -0.5 158,969 + GFF4518 0.28 +1.8 158,969 + GFF4518 0.28 +0.5 158,969 + GFF4518 0.28 +0.7 158,969 + GFF4518 0.28 +0.3 158,969 + GFF4518 0.28 -0.3 158,970 - GFF4518 0.28 -0.8 158,970 - GFF4518 0.28 +0.7 158,970 - GFF4518 0.28 +0.6 158,970 - GFF4518 0.28 +0.3 159,156 - GFF4518 0.50 -1.3 159,165 + GFF4518 0.51 -0.4 159,233 + GFF4518 0.60 +0.2 159,293 + GFF4518 0.67 +2.1 159,293 + GFF4518 0.67 -0.0 159,293 + GFF4518 0.67 +0.9 159,293 + GFF4518 0.67 +0.4 159,293 + GFF4518 0.67 -0.4 159,293 + GFF4518 0.67 +0.9 159,293 + GFF4518 0.67 -0.7 159,293 + GFF4518 0.67 +0.1 159,293 + GFF4518 0.67 -0.2 159,294 - GFF4518 0.67 -0.9 159,294 - GFF4518 0.67 -0.5 159,294 - GFF4518 0.67 +0.4 159,294 - GFF4518 0.67 +1.7 159,294 - GFF4518 0.67 -0.3 159,294 - GFF4518 0.67 +0.9 159,294 - GFF4518 0.67 +1.3 159,294 - GFF4518 0.67 -0.2 159,294 - GFF4518 0.67 -0.0 159,294 - GFF4518 0.67 +1.3 159,294 - GFF4518 0.67 +0.1 159,308 + GFF4518 0.69 -0.7 159,408 - GFF4518 0.81 -0.2 159,569 + -0.8 159,569 + +0.2 159,570 - -0.1 159,570 - +0.1 159,570 - -0.3 159,712 + GFF4519 0.36 -0.5 159,712 + GFF4519 0.36 -0.6 159,712 + GFF4519 0.36 +0.6 159,712 + GFF4519 0.36 +0.4 159,740 - GFF4519 0.44 -0.5 159,740 - GFF4519 0.44 +0.9 159,740 - GFF4519 0.44 -0.5 159,740 - GFF4519 0.44 +0.9 159,740 - GFF4519 0.44 -0.5 159,794 - GFF4519 0.60 +0.2 159,794 - GFF4519 0.60 -0.1 159,794 - GFF4519 0.60 +0.7 159,794 - GFF4519 0.60 +0.8 159,853 + GFF4519 0.77 +0.1 159,853 + GFF4519 0.77 -0.5 159,853 + GFF4519 0.77 -0.0 159,853 + GFF4519 0.77 -0.2 159,853 + GFF4519 0.77 -0.6 159,853 + GFF4519 0.77 +0.2 159,853 + GFF4519 0.77 -0.7 159,853 + GFF4519 0.77 +0.9 159,853 + GFF4519 0.77 -0.1 159,854 - GFF4519 0.78 -0.1 159,854 - GFF4519 0.78 -0.7 159,854 - GFF4519 0.78 -0.2 159,854 - GFF4519 0.78 -0.3 159,857 + GFF4519 0.79 +0.8 159,857 + GFF4519 0.79 +0.2 159,857 + GFF4519 0.79 -0.2 159,857 + GFF4519 0.79 +0.6 159,857 + GFF4519 0.79 -0.1 159,858 - GFF4519 0.79 -0.7 159,858 - GFF4519 0.79 +0.9 159,858 - GFF4519 0.79 -1.0 159,874 - GFF4519 0.84 -0.2 159,874 - GFF4519 0.84 -0.0 159,922 + +0.7 159,936 + -0.7 159,937 - -0.2 160,114 + GFF4520 0.34 -0.7 160,114 + GFF4520 0.34 -1.2 160,114 + GFF4520 0.34 -1.2 160,114 + GFF4520 0.34 -1.7 160,115 - GFF4520 0.35 -0.2 160,115 - GFF4520 0.35 +0.0 160,132 + GFF4520 0.39 -0.4 160,132 + GFF4520 0.39 -0.7 160,132 + GFF4520 0.39 +0.2 160,132 + GFF4520 0.39 -1.1 160,133 - GFF4520 0.39 -0.5 160,133 - GFF4520 0.39 -1.1 160,133 - GFF4520 0.39 -0.5 160,133 - GFF4520 0.39 -1.0 160,133 - GFF4520 0.39 +0.9 160,213 + GFF4520 0.58 +0.3 160,213 + GFF4520 0.58 +0.1 160,214 - GFF4520 0.58 -0.3 160,214 - GFF4520 0.58 +0.1 160,214 - GFF4520 0.58 -0.0 160,228 - GFF4520 0.61 +0.8 160,262 - GFF4520 0.69 +0.2 160,268 - GFF4520 0.71 +0.9 160,268 - GFF4520 0.71 -0.6 160,348 + GFF4520 0.90 -1.0 160,348 + GFF4520 0.90 -0.7 160,348 + GFF4520 0.90 -1.3 160,348 + GFF4520 0.90 +0.2 160,349 - +1.7 160,349 - -2.3 160,349 - +0.5 160,386 - -0.1 160,386 - +1.7 160,386 - +0.7 160,386 - -0.7 160,386 - +0.1
Or see this region's nucleotide sequence