Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4461 and GFF4462 are separated by 66 nucleotides GFF4462 and GFF4463 are separated by 1 nucleotides
GFF4461 - hypothetical protein, at 101,487 to 103,649
GFF4461
GFF4462 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 103,716 to 104,264
GFF4462
GFF4463 - alpha-methyl-L-serine aldolase, at 104,266 to 105,534
GFF4463
Position (kb) 103
104
105 Strain fitness (log2 ratio) -2
-1
0
1 at 103.267 kb on + strand, within GFF4461 at 103.563 kb on + strand at 103.563 kb on + strand at 103.564 kb on - strand at 103.619 kb on - strand at 103.702 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.795 kb on - strand, within GFF4462 at 104.025 kb on + strand, within GFF4462 at 104.241 kb on + strand at 104.241 kb on + strand at 104.279 kb on + strand at 104.279 kb on + strand at 104.279 kb on + strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.660 kb on + strand, within GFF4463 at 104.660 kb on + strand, within GFF4463 at 104.660 kb on + strand, within GFF4463 at 104.661 kb on - strand, within GFF4463 at 104.661 kb on - strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 105.062 kb on + strand, within GFF4463 at 105.206 kb on + strand, within GFF4463 at 105.206 kb on + strand, within GFF4463 at 105.207 kb on - strand, within GFF4463 at 105.207 kb on - strand, within GFF4463
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 103,267 + GFF4461 0.82 -1.2 103,563 + +0.1 103,563 + +1.2 103,564 - +0.1 103,619 - +0.5 103,702 + -0.0 103,711 + +0.3 103,711 + +0.1 103,711 + -0.3 103,711 + +0.2 103,712 - -1.5 103,712 - +1.2 103,712 - +0.3 103,712 - -0.8 103,712 - -0.3 103,712 - +0.8 103,795 - GFF4462 0.14 -1.3 104,025 + GFF4462 0.56 -2.1 104,241 + +0.1 104,241 + -0.2 104,279 + -0.3 104,279 + +0.3 104,279 + -0.1 104,280 - -0.8 104,280 - +0.7 104,280 - -0.3 104,280 - -0.0 104,660 + GFF4463 0.31 +0.0 104,660 + GFF4463 0.31 -0.2 104,660 + GFF4463 0.31 -0.4 104,661 - GFF4463 0.31 -1.8 104,661 - GFF4463 0.31 -1.3 104,828 + GFF4463 0.44 -0.3 104,828 + GFF4463 0.44 +0.3 104,828 + GFF4463 0.44 -0.8 104,828 + GFF4463 0.44 -1.0 104,829 - GFF4463 0.44 -1.0 104,829 - GFF4463 0.44 +0.1 104,829 - GFF4463 0.44 +0.5 105,062 + GFF4463 0.63 -0.8 105,206 + GFF4463 0.74 -1.0 105,206 + GFF4463 0.74 +0.7 105,207 - GFF4463 0.74 -0.0 105,207 - GFF4463 0.74 +0.2
Or see this region's nucleotide sequence