Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4445 and GFF4446 are separated by 4 nucleotides GFF4446 and GFF4447 are separated by 6 nucleotides
GFF4445 - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-me so-diaminopimelate ligase (EC 6.3.2.-), at 84,341 to 85,744
GFF4445
GFF4446 - FIG018171: hypothetical protein of Cupin superfamily, at 85,749 to 86,270
GFF4446
GFF4447 - 2-keto-3-deoxy-D-arabino-heptulosonate-7-phosphat e synthase I alpha (EC 2.5.1.54), at 86,277 to 87,395
GFF4447
Position (kb)
85
86
87 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 84.780 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.848 kb on + strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.849 kb on - strand, within GFF4445 at 84.890 kb on + strand, within GFF4445 at 84.890 kb on + strand, within GFF4445 at 84.891 kb on - strand, within GFF4445 at 84.891 kb on - strand, within GFF4445 at 84.891 kb on - strand, within GFF4445 at 84.976 kb on - strand, within GFF4445 at 85.346 kb on + strand, within GFF4445 at 85.346 kb on + strand, within GFF4445 at 85.346 kb on + strand, within GFF4445 at 85.347 kb on - strand, within GFF4445 at 85.347 kb on - strand, within GFF4445 at 85.347 kb on - strand, within GFF4445 at 85.758 kb on - strand at 85.758 kb on - strand at 85.758 kb on - strand at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.804 kb on + strand, within GFF4446 at 85.805 kb on - strand, within GFF4446 at 85.805 kb on - strand, within GFF4446 at 85.805 kb on - strand, within GFF4446 at 85.805 kb on - strand, within GFF4446 at 85.805 kb on - strand, within GFF4446 at 85.805 kb on - strand, within GFF4446 at 85.830 kb on + strand, within GFF4446 at 85.830 kb on + strand, within GFF4446 at 85.830 kb on + strand, within GFF4446 at 85.830 kb on + strand, within GFF4446 at 85.830 kb on + strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.831 kb on - strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.908 kb on + strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 85.909 kb on - strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.145 kb on + strand, within GFF4446 at 86.146 kb on - strand, within GFF4446 at 86.146 kb on - strand, within GFF4446 at 86.146 kb on - strand, within GFF4446 at 86.146 kb on - strand, within GFF4446 at 86.371 kb on + strand at 86.371 kb on + strand at 86.372 kb on - strand at 86.372 kb on - strand at 86.621 kb on - strand, within GFF4447 at 86.621 kb on - strand, within GFF4447 at 86.621 kb on - strand, within GFF4447 at 86.621 kb on - strand, within GFF4447 at 86.706 kb on + strand, within GFF4447 at 86.706 kb on + strand, within GFF4447 at 86.706 kb on + strand, within GFF4447 at 86.707 kb on - strand, within GFF4447 at 86.707 kb on - strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.887 kb on + strand, within GFF4447 at 86.888 kb on - strand, within GFF4447 at 86.888 kb on - strand, within GFF4447 at 86.888 kb on - strand, within GFF4447 at 86.888 kb on - strand, within GFF4447 at 86.888 kb on - strand, within GFF4447 at 86.888 kb on - strand, within GFF4447 at 87.007 kb on + strand, within GFF4447 at 87.007 kb on + strand, within GFF4447 at 87.008 kb on - strand, within GFF4447 at 87.008 kb on - strand, within GFF4447 at 87.008 kb on - strand, within GFF4447 at 87.008 kb on - strand, within GFF4447 at 87.057 kb on + strand, within GFF4447 at 87.061 kb on + strand, within GFF4447 at 87.094 kb on + strand, within GFF4447 at 87.094 kb on + strand, within GFF4447 at 87.094 kb on + strand, within GFF4447 at 87.095 kb on - strand, within GFF4447 at 87.095 kb on - strand, within GFF4447 at 87.127 kb on + strand, within GFF4447 at 87.127 kb on + strand, within GFF4447 at 87.128 kb on - strand, within GFF4447 at 87.128 kb on - strand, within GFF4447
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 84,780 + GFF4445 0.31 -0.9 84,848 + GFF4445 0.36 -0.9 84,848 + GFF4445 0.36 -1.5 84,848 + GFF4445 0.36 +0.2 84,848 + GFF4445 0.36 -1.1 84,848 + GFF4445 0.36 +1.7 84,848 + GFF4445 0.36 +0.2 84,848 + GFF4445 0.36 +0.4 84,848 + GFF4445 0.36 -0.2 84,849 - GFF4445 0.36 +0.8 84,849 - GFF4445 0.36 +0.2 84,849 - GFF4445 0.36 -1.5 84,849 - GFF4445 0.36 -1.8 84,849 - GFF4445 0.36 -0.3 84,849 - GFF4445 0.36 -0.9 84,849 - GFF4445 0.36 -0.5 84,849 - GFF4445 0.36 +1.7 84,849 - GFF4445 0.36 -0.7 84,849 - GFF4445 0.36 -0.6 84,890 + GFF4445 0.39 +0.3 84,890 + GFF4445 0.39 +0.3 84,891 - GFF4445 0.39 -0.5 84,891 - GFF4445 0.39 -0.1 84,891 - GFF4445 0.39 -0.2 84,976 - GFF4445 0.45 -0.8 85,346 + GFF4445 0.72 -0.3 85,346 + GFF4445 0.72 +0.4 85,346 + GFF4445 0.72 -1.1 85,347 - GFF4445 0.72 +0.4 85,347 - GFF4445 0.72 -0.4 85,347 - GFF4445 0.72 -0.2 85,758 - -0.2 85,758 - +0.2 85,758 - +0.1 85,804 + GFF4446 0.11 +0.2 85,804 + GFF4446 0.11 +1.0 85,804 + GFF4446 0.11 -0.8 85,804 + GFF4446 0.11 +0.7 85,804 + GFF4446 0.11 -0.2 85,804 + GFF4446 0.11 +0.7 85,804 + GFF4446 0.11 +0.7 85,804 + GFF4446 0.11 -1.8 85,804 + GFF4446 0.11 -0.6 85,805 - GFF4446 0.11 +0.3 85,805 - GFF4446 0.11 +1.2 85,805 - GFF4446 0.11 -0.3 85,805 - GFF4446 0.11 -0.3 85,805 - GFF4446 0.11 -0.5 85,805 - GFF4446 0.11 -0.6 85,830 + GFF4446 0.16 +0.2 85,830 + GFF4446 0.16 -0.0 85,830 + GFF4446 0.16 -0.1 85,830 + GFF4446 0.16 -0.3 85,830 + GFF4446 0.16 -0.3 85,831 - GFF4446 0.16 +0.6 85,831 - GFF4446 0.16 +0.5 85,831 - GFF4446 0.16 +0.7 85,831 - GFF4446 0.16 +0.7 85,831 - GFF4446 0.16 +0.7 85,831 - GFF4446 0.16 -0.3 85,831 - GFF4446 0.16 -0.3 85,831 - GFF4446 0.16 -0.9 85,908 + GFF4446 0.30 +0.7 85,908 + GFF4446 0.30 +0.2 85,908 + GFF4446 0.30 -0.2 85,908 + GFF4446 0.30 +0.3 85,908 + GFF4446 0.30 -0.3 85,908 + GFF4446 0.30 -0.2 85,908 + GFF4446 0.30 -0.8 85,908 + GFF4446 0.30 +0.3 85,908 + GFF4446 0.30 +2.1 85,908 + GFF4446 0.30 -0.1 85,908 + GFF4446 0.30 +0.2 85,908 + GFF4446 0.30 +1.7 85,909 - GFF4446 0.31 -0.2 85,909 - GFF4446 0.31 +0.1 85,909 - GFF4446 0.31 +1.6 85,909 - GFF4446 0.31 +0.7 85,909 - GFF4446 0.31 -0.7 85,909 - GFF4446 0.31 -1.3 85,909 - GFF4446 0.31 -0.9 85,909 - GFF4446 0.31 -0.6 85,909 - GFF4446 0.31 -0.5 85,909 - GFF4446 0.31 +0.3 85,909 - GFF4446 0.31 -0.2 85,909 - GFF4446 0.31 -0.8 85,909 - GFF4446 0.31 -2.2 85,909 - GFF4446 0.31 +0.1 85,909 - GFF4446 0.31 -0.1 85,909 - GFF4446 0.31 -2.1 85,909 - GFF4446 0.31 -0.4 86,145 + GFF4446 0.76 +0.2 86,145 + GFF4446 0.76 -0.5 86,145 + GFF4446 0.76 -0.3 86,145 + GFF4446 0.76 +2.7 86,145 + GFF4446 0.76 +0.5 86,145 + GFF4446 0.76 -0.0 86,145 + GFF4446 0.76 -0.8 86,146 - GFF4446 0.76 -0.8 86,146 - GFF4446 0.76 +0.4 86,146 - GFF4446 0.76 -1.2 86,146 - GFF4446 0.76 +2.0 86,371 + +0.2 86,371 + -0.8 86,372 - -2.0 86,372 - -0.7 86,621 - GFF4447 0.31 +0.3 86,621 - GFF4447 0.31 -1.3 86,621 - GFF4447 0.31 -1.5 86,621 - GFF4447 0.31 -1.4 86,706 + GFF4447 0.38 +0.7 86,706 + GFF4447 0.38 -1.1 86,706 + GFF4447 0.38 -1.3 86,707 - GFF4447 0.38 +0.4 86,707 - GFF4447 0.38 -3.8 86,887 + GFF4447 0.55 -0.3 86,887 + GFF4447 0.55 -0.7 86,887 + GFF4447 0.55 -1.5 86,887 + GFF4447 0.55 -0.9 86,887 + GFF4447 0.55 -2.5 86,887 + GFF4447 0.55 -1.0 86,887 + GFF4447 0.55 -0.9 86,887 + GFF4447 0.55 -1.6 86,888 - GFF4447 0.55 -1.3 86,888 - GFF4447 0.55 -2.1 86,888 - GFF4447 0.55 -2.2 86,888 - GFF4447 0.55 -1.1 86,888 - GFF4447 0.55 -0.9 86,888 - GFF4447 0.55 -1.9 87,007 + GFF4447 0.65 -3.7 87,007 + GFF4447 0.65 +0.3 87,008 - GFF4447 0.65 -0.5 87,008 - GFF4447 0.65 -1.6 87,008 - GFF4447 0.65 -1.8 87,008 - GFF4447 0.65 -0.8 87,057 + GFF4447 0.70 +1.7 87,061 + GFF4447 0.70 -1.9 87,094 + GFF4447 0.73 -2.4 87,094 + GFF4447 0.73 -1.9 87,094 + GFF4447 0.73 -1.3 87,095 - GFF4447 0.73 -1.3 87,095 - GFF4447 0.73 -1.6 87,127 + GFF4447 0.76 -0.0 87,127 + GFF4447 0.76 -0.5 87,128 - GFF4447 0.76 -2.1 87,128 - GFF4447 0.76 -0.1
Or see this region's nucleotide sequence