Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF438 and GFF439 overlap by 4 nucleotides GFF439 and GFF440 are separated by 21 nucleotides
GFF437 - Antiholin-like protein LrgA, at 474,788 to 475,210
GFF437
GFF438 - L-threonine 3-O-phosphate decarboxylase (EC 4.1.1.81), at 475,171 to 476,187
GFF438
GFF439 - Adenosylcobinamide-phosphate synthase (EC 6.3.1.10), at 476,184 to 477,161
GFF439
GFF440 - CobN-like chelatase BtuS for metalloporphyrine salvage, at 477,183 to 481,196
GFF440
Position (kb)
476
477
478 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 475.337 kb on + strand, within GFF438 at 475.337 kb on + strand, within GFF438 at 475.338 kb on - strand, within GFF438 at 475.595 kb on + strand, within GFF438 at 475.595 kb on + strand, within GFF438 at 475.709 kb on + strand, within GFF438 at 475.709 kb on + strand, within GFF438 at 475.710 kb on - strand, within GFF438 at 475.710 kb on - strand, within GFF438 at 475.710 kb on - strand, within GFF438 at 475.889 kb on + strand, within GFF438 at 475.890 kb on - strand, within GFF438 at 475.890 kb on - strand, within GFF438 at 475.890 kb on - strand, within GFF438 at 475.890 kb on - strand, within GFF438 at 475.890 kb on - strand, within GFF438 at 476.039 kb on + strand, within GFF438 at 476.039 kb on + strand, within GFF438 at 476.039 kb on + strand, within GFF438 at 476.054 kb on + strand, within GFF438 at 476.055 kb on - strand, within GFF438 at 476.323 kb on + strand, within GFF439 at 476.323 kb on + strand, within GFF439 at 476.323 kb on + strand, within GFF439 at 476.324 kb on - strand, within GFF439 at 476.324 kb on - strand, within GFF439 at 476.561 kb on - strand, within GFF439 at 476.596 kb on + strand, within GFF439 at 476.597 kb on - strand, within GFF439 at 476.799 kb on + strand, within GFF439 at 476.800 kb on - strand, within GFF439 at 476.800 kb on - strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.034 kb on + strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.035 kb on - strand, within GFF439 at 477.064 kb on + strand at 477.065 kb on - strand at 477.065 kb on - strand at 477.136 kb on + strand at 477.136 kb on + strand at 477.137 kb on - strand at 477.137 kb on - strand at 477.302 kb on + strand at 477.520 kb on + strand at 477.520 kb on + strand at 477.520 kb on + strand at 477.520 kb on + strand at 477.520 kb on + strand at 477.520 kb on + strand at 477.521 kb on - strand at 477.521 kb on - strand at 477.565 kb on + strand at 477.565 kb on + strand at 477.566 kb on - strand at 477.566 kb on - strand at 477.566 kb on - strand at 477.566 kb on - strand at 477.658 kb on + strand, within GFF440 at 477.658 kb on + strand, within GFF440 at 477.658 kb on + strand, within GFF440 at 477.658 kb on + strand, within GFF440 at 477.658 kb on + strand, within GFF440 at 477.658 kb on + strand, within GFF440 at 477.658 kb on + strand, within GFF440 at 477.659 kb on - strand, within GFF440 at 477.659 kb on - strand, within GFF440 at 477.659 kb on - strand, within GFF440 at 477.659 kb on - strand, within GFF440 at 477.659 kb on - strand, within GFF440 at 477.659 kb on - strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.673 kb on + strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.674 kb on - strand, within GFF440 at 477.782 kb on - strand, within GFF440 at 477.823 kb on + strand, within GFF440 at 477.904 kb on + strand, within GFF440 at 477.904 kb on + strand, within GFF440 at 477.905 kb on - strand, within GFF440 at 477.905 kb on - strand, within GFF440 at 477.961 kb on + strand, within GFF440 at 477.994 kb on + strand, within GFF440 at 478.091 kb on - strand, within GFF440 at 478.091 kb on - strand, within GFF440 at 478.114 kb on + strand, within GFF440 at 478.114 kb on + strand, within GFF440 at 478.114 kb on + strand, within GFF440 at 478.115 kb on - strand, within GFF440 at 478.115 kb on - strand, within GFF440
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 475,337 + GFF438 0.16 -1.2 475,337 + GFF438 0.16 -0.4 475,338 - GFF438 0.16 -0.4 475,595 + GFF438 0.42 +0.1 475,595 + GFF438 0.42 -0.4 475,709 + GFF438 0.53 +0.5 475,709 + GFF438 0.53 +0.1 475,710 - GFF438 0.53 -0.8 475,710 - GFF438 0.53 -0.7 475,710 - GFF438 0.53 -0.8 475,889 + GFF438 0.71 -0.1 475,890 - GFF438 0.71 +0.2 475,890 - GFF438 0.71 -0.3 475,890 - GFF438 0.71 -0.1 475,890 - GFF438 0.71 -0.3 475,890 - GFF438 0.71 +0.9 476,039 + GFF438 0.85 +0.2 476,039 + GFF438 0.85 -0.1 476,039 + GFF438 0.85 -0.1 476,054 + GFF438 0.87 +0.6 476,055 - GFF438 0.87 +0.9 476,323 + GFF439 0.14 +0.1 476,323 + GFF439 0.14 -1.1 476,323 + GFF439 0.14 -0.4 476,324 - GFF439 0.14 +0.0 476,324 - GFF439 0.14 -0.1 476,561 - GFF439 0.39 +1.6 476,596 + GFF439 0.42 -0.5 476,597 - GFF439 0.42 +2.4 476,799 + GFF439 0.63 +0.2 476,800 - GFF439 0.63 -0.3 476,800 - GFF439 0.63 +0.9 477,034 + GFF439 0.87 +0.0 477,034 + GFF439 0.87 -0.5 477,034 + GFF439 0.87 +0.0 477,034 + GFF439 0.87 -0.1 477,034 + GFF439 0.87 -0.6 477,034 + GFF439 0.87 +0.3 477,034 + GFF439 0.87 +0.2 477,035 - GFF439 0.87 -0.3 477,035 - GFF439 0.87 +0.3 477,035 - GFF439 0.87 +0.9 477,035 - GFF439 0.87 -1.5 477,035 - GFF439 0.87 -0.8 477,035 - GFF439 0.87 +0.5 477,035 - GFF439 0.87 +1.0 477,035 - GFF439 0.87 +2.2 477,064 + -0.7 477,065 - +2.2 477,065 - -0.2 477,136 + -0.6 477,136 + -0.5 477,137 - +0.8 477,137 - -0.1 477,302 + +0.5 477,520 + +1.2 477,520 + -0.0 477,520 + -0.2 477,520 + +0.1 477,520 + -0.5 477,520 + +0.7 477,521 - -0.7 477,521 - -0.6 477,565 + -1.3 477,565 + -1.4 477,566 - -0.9 477,566 - +0.2 477,566 - +0.0 477,566 - -1.1 477,658 + GFF440 0.12 +0.2 477,658 + GFF440 0.12 -0.5 477,658 + GFF440 0.12 -0.1 477,658 + GFF440 0.12 -0.4 477,658 + GFF440 0.12 +0.2 477,658 + GFF440 0.12 -0.5 477,658 + GFF440 0.12 +0.6 477,659 - GFF440 0.12 -0.4 477,659 - GFF440 0.12 -0.3 477,659 - GFF440 0.12 -1.1 477,659 - GFF440 0.12 -0.1 477,659 - GFF440 0.12 -0.0 477,659 - GFF440 0.12 -0.1 477,673 + GFF440 0.12 -0.1 477,673 + GFF440 0.12 -0.9 477,673 + GFF440 0.12 -1.1 477,673 + GFF440 0.12 -1.0 477,673 + GFF440 0.12 +0.1 477,673 + GFF440 0.12 -0.0 477,673 + GFF440 0.12 +1.0 477,673 + GFF440 0.12 -0.6 477,673 + GFF440 0.12 +0.6 477,673 + GFF440 0.12 -1.0 477,673 + GFF440 0.12 +0.2 477,673 + GFF440 0.12 -0.0 477,673 + GFF440 0.12 -0.9 477,673 + GFF440 0.12 -0.2 477,674 - GFF440 0.12 -0.7 477,674 - GFF440 0.12 +0.9 477,674 - GFF440 0.12 -0.6 477,674 - GFF440 0.12 -1.9 477,674 - GFF440 0.12 -0.1 477,674 - GFF440 0.12 +0.1 477,674 - GFF440 0.12 +1.2 477,674 - GFF440 0.12 +0.7 477,674 - GFF440 0.12 -0.4 477,674 - GFF440 0.12 -0.3 477,674 - GFF440 0.12 -0.6 477,674 - GFF440 0.12 -0.3 477,782 - GFF440 0.15 +0.4 477,823 + GFF440 0.16 +0.1 477,904 + GFF440 0.18 -0.4 477,904 + GFF440 0.18 +1.0 477,905 - GFF440 0.18 -0.3 477,905 - GFF440 0.18 -0.4 477,961 + GFF440 0.19 -0.2 477,994 + GFF440 0.20 -1.4 478,091 - GFF440 0.23 +0.7 478,091 - GFF440 0.23 +0.0 478,114 + GFF440 0.23 -0.6 478,114 + GFF440 0.23 -0.8 478,114 + GFF440 0.23 +0.0 478,115 - GFF440 0.23 +1.0 478,115 - GFF440 0.23 +0.2
Or see this region's nucleotide sequence