Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF431 and GFF432 are separated by 181 nucleotides GFF432 and GFF433 are separated by 220 nucleotides GFF433 and GFF434 are separated by 8 nucleotides
GFF431 - Esterase/lipase, at 469,798 to 470,694
GFF431
GFF432 - Probable transcription regulator protein of MDR efflux pump cluster, at 470,876 to 471,790
GFF432
GFF433 - Flagellar brake protein YcgR, at 472,011 to 472,766
GFF433
GFF434 - OsmC/Ohr family protein, at 472,775 to 473,239
GFF434
Position (kb)
470
471
472 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 469.923 kb on - strand, within GFF431 at 469.923 kb on - strand, within GFF431 at 470.325 kb on - strand, within GFF431 at 470.367 kb on - strand, within GFF431 at 470.531 kb on - strand, within GFF431 at 470.576 kb on + strand, within GFF431 at 470.728 kb on + strand at 470.729 kb on - strand at 470.729 kb on - strand at 470.729 kb on - strand at 470.729 kb on - strand at 470.729 kb on - strand at 470.730 kb on + strand at 470.730 kb on + strand at 470.738 kb on + strand at 470.738 kb on + strand at 470.739 kb on - strand at 470.739 kb on - strand at 470.739 kb on - strand at 470.739 kb on - strand at 470.739 kb on - strand at 471.068 kb on + strand, within GFF432 at 471.068 kb on + strand, within GFF432 at 471.068 kb on + strand, within GFF432 at 471.068 kb on + strand, within GFF432 at 471.069 kb on - strand, within GFF432 at 471.069 kb on - strand, within GFF432 at 471.386 kb on + strand, within GFF432 at 471.386 kb on + strand, within GFF432 at 471.386 kb on + strand, within GFF432 at 471.386 kb on + strand, within GFF432 at 471.387 kb on - strand, within GFF432 at 471.387 kb on - strand, within GFF432 at 471.387 kb on - strand, within GFF432 at 471.399 kb on - strand, within GFF432 at 471.399 kb on - strand, within GFF432 at 471.452 kb on + strand, within GFF432 at 471.452 kb on + strand, within GFF432 at 471.452 kb on + strand, within GFF432 at 471.452 kb on + strand, within GFF432 at 471.452 kb on + strand, within GFF432 at 471.452 kb on + strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.453 kb on - strand, within GFF432 at 471.480 kb on - strand, within GFF432 at 471.531 kb on - strand, within GFF432 at 471.696 kb on - strand, within GFF432 at 471.834 kb on + strand at 471.834 kb on + strand at 471.834 kb on + strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.835 kb on - strand at 471.851 kb on + strand at 471.851 kb on + strand at 471.851 kb on + strand at 471.875 kb on + strand at 471.876 kb on - strand at 471.883 kb on + strand at 471.883 kb on + strand at 471.883 kb on + strand at 471.883 kb on + strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.884 kb on - strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.994 kb on + strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.995 kb on - strand at 471.996 kb on - strand at 472.016 kb on + strand at 472.016 kb on + strand at 472.016 kb on + strand at 472.017 kb on - strand at 472.017 kb on - strand at 472.017 kb on - strand at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.146 kb on + strand, within GFF433 at 472.147 kb on - strand, within GFF433 at 472.147 kb on - strand, within GFF433 at 472.380 kb on + strand, within GFF433 at 472.380 kb on + strand, within GFF433 at 472.380 kb on + strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.381 kb on - strand, within GFF433 at 472.399 kb on - strand, within GFF433 at 472.399 kb on - strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.657 kb on + strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.658 kb on - strand, within GFF433 at 472.663 kb on - strand, within GFF433 at 472.663 kb on - strand, within GFF433 at 472.663 kb on - strand, within GFF433 at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.779 kb on + strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.780 kb on - strand at 472.780 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 469,923 - GFF431 0.14 +0.7 469,923 - GFF431 0.14 +1.8 470,325 - GFF431 0.59 +2.4 470,367 - GFF431 0.63 +0.4 470,531 - GFF431 0.82 +0.3 470,576 + GFF431 0.87 -0.2 470,728 + -0.9 470,729 - -2.3 470,729 - +0.5 470,729 - -1.4 470,729 - -0.5 470,729 - -0.4 470,730 + -1.1 470,730 + +1.2 470,738 + -0.0 470,738 + -1.0 470,739 - +0.2 470,739 - +0.4 470,739 - +0.5 470,739 - -0.3 470,739 - -0.1 471,068 + GFF432 0.21 -0.3 471,068 + GFF432 0.21 -0.7 471,068 + GFF432 0.21 -0.7 471,068 + GFF432 0.21 -1.0 471,069 - GFF432 0.21 -0.0 471,069 - GFF432 0.21 -0.1 471,386 + GFF432 0.56 +0.2 471,386 + GFF432 0.56 +0.1 471,386 + GFF432 0.56 +0.8 471,386 + GFF432 0.56 +1.6 471,387 - GFF432 0.56 -0.5 471,387 - GFF432 0.56 +1.2 471,387 - GFF432 0.56 -0.3 471,399 - GFF432 0.57 +0.2 471,399 - GFF432 0.57 -0.8 471,452 + GFF432 0.63 -1.3 471,452 + GFF432 0.63 -1.0 471,452 + GFF432 0.63 -0.0 471,452 + GFF432 0.63 +0.2 471,452 + GFF432 0.63 -2.1 471,452 + GFF432 0.63 -0.6 471,453 - GFF432 0.63 +0.6 471,453 - GFF432 0.63 +0.2 471,453 - GFF432 0.63 -0.3 471,453 - GFF432 0.63 -1.6 471,453 - GFF432 0.63 +0.1 471,453 - GFF432 0.63 -0.0 471,453 - GFF432 0.63 +0.1 471,453 - GFF432 0.63 +0.7 471,480 - GFF432 0.66 +0.8 471,531 - GFF432 0.72 -1.5 471,696 - GFF432 0.90 -0.1 471,834 + -0.4 471,834 + -0.7 471,834 + -0.7 471,835 - +0.4 471,835 - -0.1 471,835 - -0.1 471,835 - +0.6 471,835 - -0.4 471,835 - -0.2 471,851 + +0.0 471,851 + -0.7 471,851 + -0.0 471,875 + -0.5 471,876 - -1.0 471,883 + -0.1 471,883 + +0.1 471,883 + -0.2 471,883 + +0.6 471,884 - -0.1 471,884 - +1.2 471,884 - +0.0 471,884 - -1.6 471,884 - +1.0 471,884 - +0.2 471,884 - -0.5 471,994 + +0.2 471,994 + +0.0 471,994 + +0.7 471,994 + +1.2 471,994 + -0.8 471,994 + -1.0 471,994 + +1.2 471,994 + +0.1 471,994 + +0.1 471,994 + -0.4 471,994 + -0.4 471,994 + +0.3 471,994 + -0.8 471,994 + -0.7 471,994 + +0.2 471,994 + +0.3 471,994 + -0.4 471,994 + -0.6 471,994 + -0.9 471,994 + -0.5 471,994 + -0.1 471,994 + -0.6 471,995 - +0.1 471,995 - -0.4 471,995 - -0.3 471,995 - -0.4 471,995 - -0.1 471,995 - +0.2 471,995 - -0.4 471,995 - +0.6 471,995 - -0.9 471,995 - -0.3 471,995 - -0.5 471,995 - -0.1 471,995 - -0.6 471,995 - -0.2 471,995 - +0.2 471,995 - -0.0 471,995 - +1.2 471,995 - +1.2 471,995 - +0.2 471,995 - -0.1 471,995 - -0.1 471,995 - -1.0 471,995 - -0.2 471,995 - -0.5 471,995 - -0.8 471,995 - -1.4 471,995 - -2.1 471,996 - +1.5 472,016 + -0.1 472,016 + +0.3 472,016 + +0.3 472,017 - +0.9 472,017 - -0.8 472,017 - +0.3 472,146 + GFF433 0.18 -0.2 472,146 + GFF433 0.18 -0.1 472,146 + GFF433 0.18 +0.9 472,146 + GFF433 0.18 -0.6 472,146 + GFF433 0.18 +0.2 472,146 + GFF433 0.18 -0.2 472,146 + GFF433 0.18 -0.4 472,147 - GFF433 0.18 +0.9 472,147 - GFF433 0.18 -0.5 472,380 + GFF433 0.49 -1.1 472,380 + GFF433 0.49 -1.3 472,380 + GFF433 0.49 +0.0 472,381 - GFF433 0.49 +0.9 472,381 - GFF433 0.49 -0.2 472,381 - GFF433 0.49 -0.5 472,381 - GFF433 0.49 +0.7 472,381 - GFF433 0.49 -0.4 472,381 - GFF433 0.49 -1.1 472,381 - GFF433 0.49 -0.5 472,399 - GFF433 0.51 +0.2 472,399 - GFF433 0.51 -0.2 472,657 + GFF433 0.85 +0.1 472,657 + GFF433 0.85 -2.3 472,657 + GFF433 0.85 -1.0 472,657 + GFF433 0.85 -0.5 472,657 + GFF433 0.85 -0.5 472,657 + GFF433 0.85 +0.3 472,657 + GFF433 0.85 -0.0 472,657 + GFF433 0.85 -0.5 472,658 - GFF433 0.86 -0.2 472,658 - GFF433 0.86 +0.7 472,658 - GFF433 0.86 +0.2 472,658 - GFF433 0.86 +0.0 472,658 - GFF433 0.86 -0.3 472,658 - GFF433 0.86 +0.3 472,663 - GFF433 0.86 -1.4 472,663 - GFF433 0.86 +0.3 472,663 - GFF433 0.86 +0.1 472,779 + -0.2 472,779 + -0.4 472,779 + +0.1 472,779 + -0.2 472,779 + -0.4 472,779 + +0.5 472,779 + +1.0 472,779 + +0.6 472,780 - -0.7 472,780 - +1.4 472,780 - -0.0 472,780 - +0.2 472,780 - +0.6
Or see this region's nucleotide sequence