Strain Fitness in Variovorax sp. SCN45 around GFF4301

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4300 and GFF4301 are separated by 139 nucleotidesGFF4301 and GFF4302 are separated by 42 nucleotides GFF4300 - Hydroxymethylglutaryl-CoA synthase (EC 2.3.3.10) / conserved protein associated with acetyl-CoA C-acyltransferase, at 219,210 to 220,691 GFF4300 GFF4301 - Dehydrogenases with different specificities (related to short-chain alcohol dehydrogenases), at 220,831 to 221,745 GFF4301 GFF4302 - hypothetical protein AF1210, at 221,788 to 222,720 GFF4302 Position (kb) 220 221 222Strain fitness (log2 ratio) -3 -2 -1 0 1at 220.069 kb on + strand, within GFF4300at 220.612 kb on - strandat 220.695 kb on + strandat 220.722 kb on + strandat 221.270 kb on - strand, within GFF4301at 221.594 kb on - strand, within GFF4301at 222.059 kb on - strand, within GFF4302at 222.238 kb on + strand, within GFF4302at 222.305 kb on - strand, within GFF4302at 222.322 kb on + strand, within GFF4302at 222.322 kb on + strand, within GFF4302

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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220,069 + GFF4300 0.58 -0.8
220,612 - +0.2
220,695 + -2.8
220,722 + -1.1
221,270 - GFF4301 0.48 -0.9
221,594 - GFF4301 0.83 -0.9
222,059 - GFF4302 0.29 -0.6
222,238 + GFF4302 0.48 +1.6
222,305 - GFF4302 0.55 +0.5
222,322 + GFF4302 0.57 -0.8
222,322 + GFF4302 0.57 -0.3

Or see this region's nucleotide sequence