Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4268 and GFF4269 are separated by 37 nucleotides GFF4269 and GFF4270 are separated by 83 nucleotides GFF4270 and GFF4271 are separated by 62 nucleotides
GFF4268 - Uncharacterized MFS-type transporter, at 188,589 to 189,806
GFF4268
GFF4269 - Uncharacterized SAM-dependent O-methyltransferase, at 189,844 to 190,500
GFF4269
GFF4270 - Transcriptional regulator, AcrR family, at 190,584 to 191,216
GFF4270
GFF4271 - Transcriptional regulator, LysR family, at 191,279 to 192,205
GFF4271
Position (kb) 190
191
192 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 189.897 kb on - strand at 189.930 kb on - strand, within GFF4269 at 189.930 kb on - strand, within GFF4269 at 190.013 kb on + strand, within GFF4269 at 190.223 kb on + strand, within GFF4269 at 190.223 kb on + strand, within GFF4269 at 190.223 kb on + strand, within GFF4269 at 190.224 kb on - strand, within GFF4269 at 190.224 kb on - strand, within GFF4269 at 190.224 kb on - strand, within GFF4269 at 190.224 kb on - strand, within GFF4269 at 190.296 kb on - strand, within GFF4269 at 190.353 kb on - strand, within GFF4269 at 190.761 kb on + strand, within GFF4270 at 190.767 kb on + strand, within GFF4270 at 190.767 kb on + strand, within GFF4270 at 190.966 kb on - strand, within GFF4270 at 191.190 kb on + strand at 191.281 kb on - strand at 191.281 kb on - strand at 191.789 kb on + strand, within GFF4271 at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.123 kb on + strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.124 kb on - strand at 192.182 kb on - strand at 192.182 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 189,897 - +0.4 189,930 - GFF4269 0.13 -0.1 189,930 - GFF4269 0.13 +0.1 190,013 + GFF4269 0.26 -0.5 190,223 + GFF4269 0.58 -0.4 190,223 + GFF4269 0.58 -0.2 190,223 + GFF4269 0.58 +0.0 190,224 - GFF4269 0.58 -1.8 190,224 - GFF4269 0.58 -0.5 190,224 - GFF4269 0.58 +0.0 190,224 - GFF4269 0.58 -0.5 190,296 - GFF4269 0.69 -0.4 190,353 - GFF4269 0.77 -1.4 190,761 + GFF4270 0.28 -1.1 190,767 + GFF4270 0.29 -1.7 190,767 + GFF4270 0.29 -0.3 190,966 - GFF4270 0.60 +0.2 191,190 + -0.3 191,281 - -1.1 191,281 - -0.9 191,789 + GFF4271 0.55 +0.6 192,123 + -2.9 192,123 + -0.2 192,123 + -0.5 192,123 + +1.0 192,123 + -1.4 192,124 - +0.3 192,124 - +0.7 192,124 - -1.2 192,124 - -1.6 192,182 - -0.7 192,182 - +0.3
Or see this region's nucleotide sequence