Strain Fitness in Variovorax sp. SCN45 around GFF4128

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4127 and GFF4128 are separated by 44 nucleotidesGFF4128 and GFF4129 overlap by 4 nucleotides GFF4127 - Dibenzothiophene desulfurization enzyme B, at 30,531 to 31,622 GFF4127 GFF4128 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 31,667 to 33,295 GFF4128 GFF4129 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 33,292 to 34,941 GFF4129 Position (kb) 31 32 33 34Strain fitness (log2 ratio) -1 0 1 2at 30.736 kb on - strand, within GFF4127at 30.864 kb on + strand, within GFF4127at 31.076 kb on - strand, within GFF4127at 31.755 kb on + strandat 31.755 kb on + strandat 31.755 kb on + strandat 31.756 kb on - strandat 31.756 kb on - strandat 31.896 kb on + strand, within GFF4128at 31.897 kb on - strand, within GFF4128at 31.948 kb on - strand, within GFF4128at 31.948 kb on - strand, within GFF4128at 32.041 kb on - strand, within GFF4128at 32.041 kb on - strand, within GFF4128at 32.041 kb on - strand, within GFF4128at 32.262 kb on + strand, within GFF4128at 32.263 kb on - strand, within GFF4128at 32.263 kb on - strand, within GFF4128at 32.317 kb on - strand, within GFF4128at 32.428 kb on + strand, within GFF4128at 32.436 kb on + strand, within GFF4128at 32.437 kb on - strand, within GFF4128at 32.601 kb on + strand, within GFF4128at 32.601 kb on + strand, within GFF4128at 32.640 kb on + strand, within GFF4128at 32.641 kb on - strand, within GFF4128at 32.740 kb on - strand, within GFF4128at 32.829 kb on + strand, within GFF4128at 32.829 kb on + strand, within GFF4128at 32.830 kb on - strand, within GFF4128at 32.889 kb on + strand, within GFF4128at 33.052 kb on - strand, within GFF4128at 33.052 kb on - strand, within GFF4128at 33.354 kb on + strandat 33.354 kb on + strandat 33.657 kb on - strand, within GFF4129at 33.672 kb on - strand, within GFF4129at 33.737 kb on + strand, within GFF4129at 33.738 kb on - strand, within GFF4129at 34.226 kb on + strand, within GFF4129at 34.226 kb on + strand, within GFF4129at 34.226 kb on + strand, within GFF4129at 34.226 kb on + strand, within GFF4129at 34.226 kb on + strand, within GFF4129at 34.227 kb on - strand, within GFF4129at 34.227 kb on - strand, within GFF4129at 34.247 kb on + strand, within GFF4129

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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30,736 - GFF4127 0.19 -0.2
30,864 + GFF4127 0.30 -0.4
31,076 - GFF4127 0.50 -0.3
31,755 + -0.2
31,755 + +2.2
31,755 + +0.4
31,756 - +0.5
31,756 - +0.1
31,896 + GFF4128 0.14 -0.1
31,897 - GFF4128 0.14 +0.1
31,948 - GFF4128 0.17 +0.0
31,948 - GFF4128 0.17 -0.4
32,041 - GFF4128 0.23 +1.6
32,041 - GFF4128 0.23 +0.3
32,041 - GFF4128 0.23 -1.1
32,262 + GFF4128 0.37 +0.6
32,263 - GFF4128 0.37 +0.4
32,263 - GFF4128 0.37 -0.7
32,317 - GFF4128 0.40 +1.0
32,428 + GFF4128 0.47 +1.1
32,436 + GFF4128 0.47 +2.6
32,437 - GFF4128 0.47 +1.6
32,601 + GFF4128 0.57 +0.1
32,601 + GFF4128 0.57 -0.2
32,640 + GFF4128 0.60 +0.0
32,641 - GFF4128 0.60 -0.7
32,740 - GFF4128 0.66 +1.6
32,829 + GFF4128 0.71 -0.1
32,829 + GFF4128 0.71 +0.8
32,830 - GFF4128 0.71 -1.2
32,889 + GFF4128 0.75 -1.0
33,052 - GFF4128 0.85 -0.3
33,052 - GFF4128 0.85 -0.7
33,354 + -1.6
33,354 + +0.0
33,657 - GFF4129 0.22 -1.4
33,672 - GFF4129 0.23 +0.0
33,737 + GFF4129 0.27 +0.1
33,738 - GFF4129 0.27 +0.0
34,226 + GFF4129 0.57 -0.1
34,226 + GFF4129 0.57 +0.5
34,226 + GFF4129 0.57 +0.0
34,226 + GFF4129 0.57 -0.7
34,226 + GFF4129 0.57 +1.0
34,227 - GFF4129 0.57 -0.4
34,227 - GFF4129 0.57 -0.3
34,247 + GFF4129 0.58 -0.5

Or see this region's nucleotide sequence