Strain Fitness in Variovorax sp. SCN45 around GFF4111

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4109 and GFF4110 overlap by 4 nucleotidesGFF4110 and GFF4111 are separated by 95 nucleotidesGFF4111 and GFF4112 are separated by 77 nucleotides GFF4109 - Aspartate aminotransferase (EC 2.6.1.1), at 11,910 to 13,148 GFF4109 GFF4110 - ABC-type amino acid transport/signal transduction system, periplasmic component/domain, at 13,145 to 13,969 GFF4110 GFF4111 - ABC-type amino acid transport/signal transduction system, periplasmic component/domain, at 14,065 to 14,913 GFF4111 GFF4112 - Rhodanese-related sulfurtransferase, 4 domains, at 14,991 to 16,667 GFF4112 Position (kb) 14 15Strain fitness (log2 ratio) -1 0 1at 14.409 kb on - strand, within GFF4111at 14.579 kb on + strand, within GFF4111at 14.579 kb on + strand, within GFF4111at 14.580 kb on - strand, within GFF4111at 14.996 kb on + strandat 15.002 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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14,409 - GFF4111 0.41 -0.4
14,579 + GFF4111 0.61 -0.6
14,579 + GFF4111 0.61 -0.4
14,580 - GFF4111 0.61 -1.4
14,996 + -0.2
15,002 + +1.3

Or see this region's nucleotide sequence