Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3991 and GFF3992 are separated by 157 nucleotides GFF3992 and GFF3993 are separated by 91 nucleotides GFF3993 and GFF3994 are separated by 272 nucleotides GFF3994 and GFF3995 overlap by 19 nucleotides
GFF3991 - no description, at 194,501 to 194,707
GFF3991
GFF3992 - HNH endonuclease family protein, at 194,865 to 195,428
GFF3992
GFF3993 - no description, at 195,520 to 195,696
GFF3993
GFF3994 - hypothetical protein, at 195,969 to 196,406
GFF3994
GFF3995 - hypothetical protein, at 196,388 to 197,209
GFF3995
Position (kb) 194
195
196 Strain fitness (log2 ratio) -2
-1
0
1
2 at 193.923 kb on + strand at 193.923 kb on + strand at 193.923 kb on + strand at 193.924 kb on - strand at 193.924 kb on - strand at 193.978 kb on + strand at 193.998 kb on + strand at 194.006 kb on + strand at 194.006 kb on + strand at 194.006 kb on + strand at 194.006 kb on + strand at 194.006 kb on + strand at 194.006 kb on + strand at 194.007 kb on - strand at 194.141 kb on + strand at 194.142 kb on - strand at 194.195 kb on - strand at 194.195 kb on - strand at 194.260 kb on + strand at 194.261 kb on - strand at 194.376 kb on + strand at 194.394 kb on + strand at 194.394 kb on + strand at 194.395 kb on - strand at 194.395 kb on - strand at 194.420 kb on + strand at 194.705 kb on + strand at 194.705 kb on + strand at 194.705 kb on + strand at 194.705 kb on + strand at 194.705 kb on + strand at 194.706 kb on - strand at 194.706 kb on - strand at 194.737 kb on + strand at 194.738 kb on - strand at 194.738 kb on - strand at 194.891 kb on + strand at 194.943 kb on + strand, within GFF3992 at 194.943 kb on + strand, within GFF3992 at 194.943 kb on + strand, within GFF3992 at 195.013 kb on + strand, within GFF3992 at 195.151 kb on - strand, within GFF3992 at 195.312 kb on + strand, within GFF3992 at 195.312 kb on + strand, within GFF3992 at 195.312 kb on + strand, within GFF3992 at 195.313 kb on - strand, within GFF3992 at 195.313 kb on - strand, within GFF3992 at 195.313 kb on - strand, within GFF3992 at 195.313 kb on - strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.321 kb on + strand, within GFF3992 at 195.322 kb on - strand, within GFF3992 at 195.322 kb on - strand, within GFF3992 at 195.322 kb on - strand, within GFF3992 at 195.322 kb on - strand, within GFF3992 at 195.322 kb on - strand, within GFF3992 at 195.427 kb on - strand at 195.427 kb on - strand at 195.463 kb on + strand at 195.463 kb on + strand at 195.464 kb on - strand at 195.464 kb on - strand at 195.662 kb on - strand, within GFF3993 at 195.676 kb on + strand, within GFF3993 at 195.676 kb on + strand, within GFF3993 at 195.676 kb on + strand, within GFF3993 at 195.676 kb on + strand, within GFF3993 at 195.677 kb on - strand, within GFF3993 at 195.677 kb on - strand, within GFF3993 at 195.677 kb on - strand, within GFF3993 at 195.677 kb on - strand, within GFF3993 at 195.677 kb on - strand, within GFF3993 at 195.706 kb on + strand at 195.709 kb on - strand at 195.709 kb on - strand at 195.709 kb on - strand at 195.709 kb on - strand at 195.713 kb on + strand at 195.713 kb on + strand at 195.713 kb on + strand at 195.713 kb on + strand at 195.714 kb on - strand at 195.714 kb on - strand at 195.714 kb on - strand at 195.714 kb on - strand at 195.761 kb on + strand at 195.761 kb on + strand at 195.762 kb on - strand at 195.762 kb on - strand at 195.762 kb on - strand at 195.782 kb on + strand at 195.854 kb on + strand at 195.854 kb on + strand at 195.854 kb on + strand at 195.854 kb on + strand at 195.855 kb on - strand at 195.855 kb on - strand at 195.914 kb on + strand at 195.915 kb on - strand at 195.961 kb on - strand at 195.961 kb on - strand at 195.968 kb on + strand at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.072 kb on + strand, within GFF3994 at 196.073 kb on - strand, within GFF3994 at 196.073 kb on - strand, within GFF3994 at 196.073 kb on - strand, within GFF3994 at 196.073 kb on - strand, within GFF3994 at 196.087 kb on - strand, within GFF3994 at 196.089 kb on - strand, within GFF3994 at 196.119 kb on - strand, within GFF3994 at 196.119 kb on - strand, within GFF3994 at 196.299 kb on + strand, within GFF3994 at 196.299 kb on + strand, within GFF3994 at 196.300 kb on - strand, within GFF3994 at 196.300 kb on - strand, within GFF3994 at 196.300 kb on - strand, within GFF3994 at 196.339 kb on + strand, within GFF3994 at 196.385 kb on + strand at 196.385 kb on + strand at 196.385 kb on + strand at 196.385 kb on + strand at 196.385 kb on + strand at 196.385 kb on + strand at 196.385 kb on + strand at 196.386 kb on - strand at 196.386 kb on - strand at 196.386 kb on - strand at 196.386 kb on - strand at 196.386 kb on - strand at 196.386 kb on - strand at 196.386 kb on - strand at 196.391 kb on + strand at 196.392 kb on - strand at 196.392 kb on - strand at 196.392 kb on - strand at 196.392 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 193,923 + -0.2 193,923 + +0.9 193,923 + -0.1 193,924 - +0.1 193,924 - -0.4 193,978 + -0.5 193,998 + +1.5 194,006 + -0.8 194,006 + +1.5 194,006 + +0.1 194,006 + -0.3 194,006 + +0.3 194,006 + -0.0 194,007 - -1.6 194,141 + -1.5 194,142 - +0.5 194,195 - +0.4 194,195 - -0.1 194,260 + -0.8 194,261 - -0.5 194,376 + -0.1 194,394 + +0.2 194,394 + +0.0 194,395 - -0.6 194,395 - +0.5 194,420 + -0.3 194,705 + +0.3 194,705 + -0.5 194,705 + -0.5 194,705 + +0.0 194,705 + +0.7 194,706 - +0.5 194,706 - +0.0 194,737 + +0.1 194,738 - -2.1 194,738 - -0.4 194,891 + -2.1 194,943 + GFF3992 0.14 -1.1 194,943 + GFF3992 0.14 +0.5 194,943 + GFF3992 0.14 +1.5 195,013 + GFF3992 0.26 -0.7 195,151 - GFF3992 0.51 -0.1 195,312 + GFF3992 0.79 -0.7 195,312 + GFF3992 0.79 -0.5 195,312 + GFF3992 0.79 -1.5 195,313 - GFF3992 0.79 -0.3 195,313 - GFF3992 0.79 -0.0 195,313 - GFF3992 0.79 -0.4 195,313 - GFF3992 0.79 +0.3 195,321 + GFF3992 0.81 -0.7 195,321 + GFF3992 0.81 +0.5 195,321 + GFF3992 0.81 -0.2 195,321 + GFF3992 0.81 +0.3 195,321 + GFF3992 0.81 -0.1 195,321 + GFF3992 0.81 +1.5 195,321 + GFF3992 0.81 -1.1 195,321 + GFF3992 0.81 +0.8 195,322 - GFF3992 0.81 -0.1 195,322 - GFF3992 0.81 +0.0 195,322 - GFF3992 0.81 -0.7 195,322 - GFF3992 0.81 +1.1 195,322 - GFF3992 0.81 +0.2 195,427 - -1.3 195,427 - -0.6 195,463 + -0.6 195,463 + -0.2 195,464 - -0.4 195,464 - -0.5 195,662 - GFF3993 0.80 -0.3 195,676 + GFF3993 0.88 +0.7 195,676 + GFF3993 0.88 -0.7 195,676 + GFF3993 0.88 +0.3 195,676 + GFF3993 0.88 -0.8 195,677 - GFF3993 0.89 -0.1 195,677 - GFF3993 0.89 -0.3 195,677 - GFF3993 0.89 +0.5 195,677 - GFF3993 0.89 -0.1 195,677 - GFF3993 0.89 +0.3 195,706 + -1.1 195,709 - +0.1 195,709 - -0.4 195,709 - -0.4 195,709 - +0.1 195,713 + +0.0 195,713 + -1.1 195,713 + +0.2 195,713 + +0.9 195,714 - +1.4 195,714 - -1.0 195,714 - -0.1 195,714 - -1.3 195,761 + +0.5 195,761 + -0.4 195,762 - -2.3 195,762 - -0.0 195,762 - -0.5 195,782 + +0.2 195,854 + -0.5 195,854 + -0.6 195,854 + +1.5 195,854 + -0.3 195,855 - +0.4 195,855 - +0.3 195,914 + +0.3 195,915 - -0.2 195,961 - +0.9 195,961 - +0.5 195,968 + +1.5 196,072 + GFF3994 0.24 +0.6 196,072 + GFF3994 0.24 +1.3 196,072 + GFF3994 0.24 +2.1 196,072 + GFF3994 0.24 +1.5 196,072 + GFF3994 0.24 +0.7 196,072 + GFF3994 0.24 -0.2 196,072 + GFF3994 0.24 -0.9 196,072 + GFF3994 0.24 -0.7 196,073 - GFF3994 0.24 +0.7 196,073 - GFF3994 0.24 -0.6 196,073 - GFF3994 0.24 -2.4 196,073 - GFF3994 0.24 -0.1 196,087 - GFF3994 0.27 +0.0 196,089 - GFF3994 0.27 -0.4 196,119 - GFF3994 0.34 +0.8 196,119 - GFF3994 0.34 -0.4 196,299 + GFF3994 0.75 -0.3 196,299 + GFF3994 0.75 -0.2 196,300 - GFF3994 0.76 +0.5 196,300 - GFF3994 0.76 -0.8 196,300 - GFF3994 0.76 +0.3 196,339 + GFF3994 0.84 -0.1 196,385 + -0.2 196,385 + -0.1 196,385 + +1.5 196,385 + +0.3 196,385 + +0.3 196,385 + -0.0 196,385 + -0.3 196,386 - -0.5 196,386 - +0.7 196,386 - -0.5 196,386 - +1.2 196,386 - -0.1 196,386 - -1.1 196,386 - +0.0 196,391 + -0.3 196,392 - +1.1 196,392 - -0.7 196,392 - -0.2 196,392 - -0.3
Or see this region's nucleotide sequence