Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3968 and GFF3969 are separated by 13 nucleotides GFF3969 and GFF3970 are separated by 20 nucleotides
GFF3968 - Indolepyruvate oxidoreductase subunit IorB II (EC 1.2.7.8), at 170,169 to 171,674
GFF3968
GFF3969 - Flavodoxin reductases (ferredoxin-NADPH reductases) family 1; Vanillate O-demethylase oxidoreductase (EC 1.14.13.-), at 171,688 to 172,680
GFF3969
GFF3970 - Ring hydroxylating dioxygenase, alpha subunit (EC 1.14.12.13), at 172,701 to 174,014
GFF3970
Position (kb) 171
172
173 Strain fitness (log2 ratio) -1
0
1
2 at 170.973 kb on + strand, within GFF3968 at 170.985 kb on + strand, within GFF3968 at 171.186 kb on + strand, within GFF3968 at 171.186 kb on + strand, within GFF3968 at 171.186 kb on + strand, within GFF3968 at 171.187 kb on - strand, within GFF3968 at 171.187 kb on - strand, within GFF3968 at 171.331 kb on - strand, within GFF3968 at 171.387 kb on + strand, within GFF3968 at 171.387 kb on + strand, within GFF3968 at 171.388 kb on - strand, within GFF3968 at 171.388 kb on - strand, within GFF3968 at 171.502 kb on - strand, within GFF3968 at 171.534 kb on + strand at 171.534 kb on + strand at 171.534 kb on + strand at 171.534 kb on + strand at 171.919 kb on + strand, within GFF3969 at 171.919 kb on + strand, within GFF3969 at 172.294 kb on + strand, within GFF3969 at 172.634 kb on + strand at 172.634 kb on + strand at 172.635 kb on - strand at 172.710 kb on + strand at 172.773 kb on + strand at 172.774 kb on - strand at 173.082 kb on + strand, within GFF3970 at 173.206 kb on - strand, within GFF3970 at 173.547 kb on + strand, within GFF3970 at 173.547 kb on + strand, within GFF3970 at 173.548 kb on - strand, within GFF3970 at 173.548 kb on - strand, within GFF3970
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 170,973 + GFF3968 0.53 -0.4 170,985 + GFF3968 0.54 +0.2 171,186 + GFF3968 0.68 +0.5 171,186 + GFF3968 0.68 -0.5 171,186 + GFF3968 0.68 -0.1 171,187 - GFF3968 0.68 +0.1 171,187 - GFF3968 0.68 -0.5 171,331 - GFF3968 0.77 +0.5 171,387 + GFF3968 0.81 -0.4 171,387 + GFF3968 0.81 -0.5 171,388 - GFF3968 0.81 -1.1 171,388 - GFF3968 0.81 -1.1 171,502 - GFF3968 0.89 -0.4 171,534 + -0.3 171,534 + -0.7 171,534 + -0.5 171,534 + +0.2 171,919 + GFF3969 0.23 +0.2 171,919 + GFF3969 0.23 -1.3 172,294 + GFF3969 0.61 -1.4 172,634 + +1.9 172,634 + +0.2 172,635 - -1.6 172,710 + -0.4 172,773 + -0.2 172,774 - +0.2 173,082 + GFF3970 0.29 -0.6 173,206 - GFF3970 0.38 -0.1 173,547 + GFF3970 0.64 -0.8 173,547 + GFF3970 0.64 -1.4 173,548 - GFF3970 0.64 +0.1 173,548 - GFF3970 0.64 -0.6
Or see this region's nucleotide sequence