Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3962 and GFF3963 overlap by 11 nucleotides GFF3963 and GFF3964 overlap by 4 nucleotides GFF3964 and GFF3965 overlap by 4 nucleotides
GFF3962 - Opine dehydrogenase (EC 1.5.1.28), at 163,561 to 164,634
GFF3962
GFF3963 - 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35), at 164,624 to 165,628
GFF3963
GFF3964 - 2-hydroxymuconic semialdehyde hydrolase (EC 3.7.1.9), at 165,625 to 166,428
GFF3964
GFF3965 - Alcohol dehydrogenase, zinc-binding, at 166,425 to 167,309
GFF3965
Position (kb) 164
165
166 Strain fitness (log2 ratio) -1
0
1
2 at 163.844 kb on + strand, within GFF3962 at 163.844 kb on + strand, within GFF3962 at 163.845 kb on - strand, within GFF3962 at 163.845 kb on - strand, within GFF3962 at 163.845 kb on - strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.891 kb on + strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.892 kb on - strand, within GFF3962 at 163.966 kb on + strand, within GFF3962 at 163.966 kb on + strand, within GFF3962 at 163.967 kb on - strand, within GFF3962 at 164.039 kb on - strand, within GFF3962 at 164.086 kb on + strand, within GFF3962 at 164.087 kb on - strand, within GFF3962 at 164.087 kb on - strand, within GFF3962 at 164.311 kb on + strand, within GFF3962 at 164.312 kb on - strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.329 kb on + strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.330 kb on - strand, within GFF3962 at 164.350 kb on + strand, within GFF3962 at 164.350 kb on + strand, within GFF3962 at 164.350 kb on + strand, within GFF3962 at 164.351 kb on - strand, within GFF3962 at 164.351 kb on - strand, within GFF3962 at 164.351 kb on - strand, within GFF3962 at 164.422 kb on + strand, within GFF3962 at 164.422 kb on + strand, within GFF3962 at 164.423 kb on - strand, within GFF3962 at 164.423 kb on - strand, within GFF3962 at 164.423 kb on - strand, within GFF3962 at 164.423 kb on - strand, within GFF3962 at 164.705 kb on + strand at 164.793 kb on + strand, within GFF3963 at 165.102 kb on - strand, within GFF3963 at 165.102 kb on - strand, within GFF3963 at 165.440 kb on + strand, within GFF3963 at 165.512 kb on + strand, within GFF3963 at 165.512 kb on + strand, within GFF3963 at 165.513 kb on - strand, within GFF3963 at 165.519 kb on - strand, within GFF3963 at 165.676 kb on + strand at 165.676 kb on + strand at 165.749 kb on - strand, within GFF3964 at 165.749 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 165.912 kb on - strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.003 kb on + strand, within GFF3964 at 166.004 kb on - strand, within GFF3964 at 166.594 kb on - strand, within GFF3965
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 163,844 + GFF3962 0.26 +0.0 163,844 + GFF3962 0.26 +0.6 163,845 - GFF3962 0.26 -1.3 163,845 - GFF3962 0.26 -0.9 163,845 - GFF3962 0.26 -0.7 163,891 + GFF3962 0.31 +0.4 163,891 + GFF3962 0.31 -0.6 163,891 + GFF3962 0.31 +0.3 163,891 + GFF3962 0.31 +0.1 163,892 - GFF3962 0.31 +1.3 163,892 - GFF3962 0.31 +0.2 163,892 - GFF3962 0.31 -0.9 163,892 - GFF3962 0.31 +0.0 163,892 - GFF3962 0.31 -0.5 163,966 + GFF3962 0.38 +0.0 163,966 + GFF3962 0.38 -1.1 163,967 - GFF3962 0.38 +0.3 164,039 - GFF3962 0.45 +0.3 164,086 + GFF3962 0.49 +0.5 164,087 - GFF3962 0.49 -0.2 164,087 - GFF3962 0.49 -0.7 164,311 + GFF3962 0.70 -1.1 164,312 - GFF3962 0.70 +0.1 164,329 + GFF3962 0.72 -0.5 164,329 + GFF3962 0.72 -0.3 164,329 + GFF3962 0.72 -0.1 164,329 + GFF3962 0.72 -0.3 164,330 - GFF3962 0.72 -0.0 164,330 - GFF3962 0.72 +0.7 164,330 - GFF3962 0.72 +0.3 164,330 - GFF3962 0.72 -0.1 164,350 + GFF3962 0.73 -0.8 164,350 + GFF3962 0.73 +0.2 164,350 + GFF3962 0.73 +0.8 164,351 - GFF3962 0.74 +0.1 164,351 - GFF3962 0.74 +0.1 164,351 - GFF3962 0.74 -0.6 164,422 + GFF3962 0.80 -0.1 164,422 + GFF3962 0.80 -1.3 164,423 - GFF3962 0.80 +0.5 164,423 - GFF3962 0.80 -1.5 164,423 - GFF3962 0.80 -0.8 164,423 - GFF3962 0.80 -0.2 164,705 + +1.4 164,793 + GFF3963 0.17 +0.5 165,102 - GFF3963 0.48 +0.2 165,102 - GFF3963 0.48 +0.3 165,440 + GFF3963 0.81 +1.9 165,512 + GFF3963 0.88 -0.1 165,512 + GFF3963 0.88 +1.2 165,513 - GFF3963 0.88 -1.5 165,519 - GFF3963 0.89 -0.1 165,676 + +2.0 165,676 + +0.9 165,749 - GFF3964 0.15 +0.4 165,749 - GFF3964 0.15 +0.5 165,912 - GFF3964 0.36 -0.6 165,912 - GFF3964 0.36 -0.0 165,912 - GFF3964 0.36 +0.2 166,003 + GFF3964 0.47 -0.4 166,003 + GFF3964 0.47 -0.3 166,003 + GFF3964 0.47 -0.8 166,004 - GFF3964 0.47 +0.1 166,594 - GFF3965 0.19 -0.8
Or see this region's nucleotide sequence