Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3847 and GFF3848 overlap by 8 nucleotides GFF3848 and GFF3849 are separated by 21 nucleotides GFF3849 and GFF3850 are separated by 1 nucleotides
GFF3847 - LSU rRNA pseudouridine(955/2504/2580) synthase (EC 5.4.99.24), at 47,934 to 49,046
GFF3847
GFF3848 - Similar to phosphoglycolate phosphatase, clustered with ribosomal large subunit pseudouridine synthase C, at 49,039 to 49,710
GFF3848
GFF3849 - Iron-sulfur cluster-binding protein, Rieske family, at 49,732 to 50,100
GFF3849
GFF3850 - Periplasmic serine proteases (ClpP class), at 50,102 to 51,118
GFF3850
Position (kb)
49
50 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 48.135 kb on + strand, within GFF3847 at 48.136 kb on - strand, within GFF3847 at 48.136 kb on - strand, within GFF3847 at 48.136 kb on - strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.537 kb on + strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.538 kb on - strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.603 kb on + strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.604 kb on - strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.840 kb on + strand, within GFF3847 at 48.841 kb on - strand, within GFF3847 at 48.841 kb on - strand, within GFF3847 at 48.841 kb on - strand, within GFF3847 at 48.980 kb on + strand at 48.980 kb on + strand at 48.980 kb on + strand at 48.980 kb on + strand at 48.981 kb on - strand at 48.981 kb on - strand at 48.981 kb on - strand at 48.981 kb on - strand at 48.981 kb on - strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.061 kb on + strand at 49.062 kb on - strand at 49.062 kb on - strand at 49.062 kb on - strand at 49.062 kb on - strand at 49.102 kb on + strand at 49.103 kb on - strand at 49.103 kb on - strand at 49.255 kb on + strand, within GFF3848 at 49.255 kb on + strand, within GFF3848 at 49.256 kb on - strand, within GFF3848 at 49.256 kb on - strand, within GFF3848 at 49.276 kb on + strand, within GFF3848 at 49.282 kb on + strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.283 kb on - strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.288 kb on + strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.289 kb on - strand, within GFF3848 at 49.455 kb on + strand, within GFF3848 at 49.456 kb on - strand, within GFF3848 at 49.456 kb on - strand, within GFF3848 at 49.456 kb on - strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.612 kb on + strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.613 kb on - strand, within GFF3848 at 49.695 kb on + strand at 49.695 kb on + strand at 49.696 kb on - strand at 49.696 kb on - strand at 49.972 kb on + strand, within GFF3849 at 49.972 kb on + strand, within GFF3849 at 49.972 kb on + strand, within GFF3849 at 49.972 kb on + strand, within GFF3849 at 50.067 kb on + strand at 50.067 kb on + strand at 50.067 kb on + strand at 50.067 kb on + strand at 50.067 kb on + strand at 50.068 kb on - strand at 50.068 kb on - strand at 50.068 kb on - strand at 50.068 kb on - strand at 50.068 kb on - strand at 50.068 kb on - strand at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.558 kb on + strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.559 kb on - strand, within GFF3850 at 50.573 kb on + strand, within GFF3850 at 50.573 kb on + strand, within GFF3850 at 50.573 kb on + strand, within GFF3850 at 50.573 kb on + strand, within GFF3850 at 50.573 kb on + strand, within GFF3850 at 50.573 kb on + strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.574 kb on - strand, within GFF3850 at 50.609 kb on + strand, within GFF3850 at 50.609 kb on + strand, within GFF3850 at 50.609 kb on + strand, within GFF3850 at 50.609 kb on + strand, within GFF3850 at 50.610 kb on - strand, within GFF3850 at 50.610 kb on - strand, within GFF3850 at 50.610 kb on - strand, within GFF3850 at 50.610 kb on - strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.612 kb on + strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.613 kb on - strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.639 kb on + strand, within GFF3850 at 50.640 kb on - strand, within GFF3850 at 50.640 kb on - strand, within GFF3850 at 50.640 kb on - strand, within GFF3850 at 50.640 kb on - strand, within GFF3850 at 50.640 kb on - strand, within GFF3850
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 48,135 + GFF3847 0.18 +1.5 48,136 - GFF3847 0.18 -0.1 48,136 - GFF3847 0.18 -0.1 48,136 - GFF3847 0.18 +0.2 48,537 + GFF3847 0.54 +0.7 48,537 + GFF3847 0.54 -0.3 48,537 + GFF3847 0.54 -0.2 48,537 + GFF3847 0.54 +0.8 48,537 + GFF3847 0.54 -1.2 48,538 - GFF3847 0.54 +1.3 48,538 - GFF3847 0.54 +0.5 48,538 - GFF3847 0.54 -0.3 48,538 - GFF3847 0.54 -1.4 48,538 - GFF3847 0.54 +2.3 48,538 - GFF3847 0.54 -0.4 48,538 - GFF3847 0.54 +1.0 48,538 - GFF3847 0.54 -0.7 48,538 - GFF3847 0.54 -1.1 48,538 - GFF3847 0.54 -0.1 48,538 - GFF3847 0.54 -0.1 48,538 - GFF3847 0.54 -0.7 48,538 - GFF3847 0.54 -0.8 48,603 + GFF3847 0.60 -0.3 48,603 + GFF3847 0.60 -0.4 48,603 + GFF3847 0.60 +0.6 48,603 + GFF3847 0.60 -0.8 48,603 + GFF3847 0.60 -0.4 48,603 + GFF3847 0.60 -0.9 48,603 + GFF3847 0.60 -0.1 48,603 + GFF3847 0.60 -0.0 48,603 + GFF3847 0.60 +0.4 48,603 + GFF3847 0.60 -0.3 48,604 - GFF3847 0.60 +0.7 48,604 - GFF3847 0.60 -1.2 48,604 - GFF3847 0.60 -0.8 48,604 - GFF3847 0.60 +0.2 48,604 - GFF3847 0.60 -0.4 48,840 + GFF3847 0.81 -0.1 48,840 + GFF3847 0.81 -0.1 48,840 + GFF3847 0.81 -0.8 48,840 + GFF3847 0.81 -0.6 48,840 + GFF3847 0.81 -0.3 48,840 + GFF3847 0.81 -0.9 48,840 + GFF3847 0.81 -0.3 48,840 + GFF3847 0.81 +0.4 48,841 - GFF3847 0.81 +0.4 48,841 - GFF3847 0.81 -0.2 48,841 - GFF3847 0.81 -0.6 48,980 + +0.6 48,980 + -0.8 48,980 + -0.7 48,980 + -1.9 48,981 - -0.3 48,981 - +0.3 48,981 - -0.5 48,981 - -0.5 48,981 - -0.0 49,061 + +0.2 49,061 + -1.1 49,061 + -0.3 49,061 + -1.6 49,061 + -1.1 49,061 + -1.8 49,061 + -0.1 49,062 - -0.3 49,062 - +0.5 49,062 - -0.2 49,062 - +0.1 49,102 + -1.1 49,103 - +0.4 49,103 - -1.5 49,255 + GFF3848 0.32 +0.5 49,255 + GFF3848 0.32 -0.4 49,256 - GFF3848 0.32 -0.0 49,256 - GFF3848 0.32 -0.3 49,276 + GFF3848 0.35 -1.0 49,282 + GFF3848 0.36 +0.5 49,283 - GFF3848 0.36 -0.9 49,283 - GFF3848 0.36 -0.1 49,283 - GFF3848 0.36 +0.5 49,283 - GFF3848 0.36 -1.1 49,288 + GFF3848 0.37 -1.4 49,288 + GFF3848 0.37 +0.8 49,288 + GFF3848 0.37 -0.2 49,288 + GFF3848 0.37 -1.2 49,288 + GFF3848 0.37 -0.3 49,288 + GFF3848 0.37 -0.4 49,288 + GFF3848 0.37 +0.1 49,288 + GFF3848 0.37 -0.1 49,288 + GFF3848 0.37 -1.2 49,288 + GFF3848 0.37 +0.3 49,288 + GFF3848 0.37 +0.0 49,289 - GFF3848 0.37 -0.6 49,289 - GFF3848 0.37 -1.4 49,289 - GFF3848 0.37 +0.1 49,289 - GFF3848 0.37 -0.3 49,289 - GFF3848 0.37 -0.6 49,289 - GFF3848 0.37 +1.1 49,289 - GFF3848 0.37 -0.8 49,289 - GFF3848 0.37 -0.1 49,289 - GFF3848 0.37 -0.4 49,289 - GFF3848 0.37 +0.0 49,289 - GFF3848 0.37 -0.8 49,289 - GFF3848 0.37 -1.4 49,455 + GFF3848 0.62 -1.8 49,456 - GFF3848 0.62 +0.9 49,456 - GFF3848 0.62 +0.4 49,456 - GFF3848 0.62 -1.3 49,612 + GFF3848 0.85 +1.1 49,612 + GFF3848 0.85 -0.2 49,612 + GFF3848 0.85 +0.8 49,612 + GFF3848 0.85 -0.1 49,612 + GFF3848 0.85 -0.6 49,612 + GFF3848 0.85 -0.2 49,612 + GFF3848 0.85 -0.0 49,612 + GFF3848 0.85 -1.8 49,612 + GFF3848 0.85 +0.6 49,613 - GFF3848 0.85 +0.3 49,613 - GFF3848 0.85 -0.6 49,613 - GFF3848 0.85 -1.7 49,613 - GFF3848 0.85 -0.3 49,613 - GFF3848 0.85 -0.2 49,613 - GFF3848 0.85 -0.2 49,613 - GFF3848 0.85 -0.9 49,613 - GFF3848 0.85 -0.0 49,613 - GFF3848 0.85 +1.3 49,613 - GFF3848 0.85 -0.7 49,613 - GFF3848 0.85 +0.4 49,695 + +0.1 49,695 + +1.2 49,696 - -0.6 49,696 - -0.7 49,972 + GFF3849 0.65 +0.1 49,972 + GFF3849 0.65 +0.2 49,972 + GFF3849 0.65 -0.3 49,972 + GFF3849 0.65 -0.9 50,067 + -0.6 50,067 + +0.7 50,067 + +1.4 50,067 + -1.3 50,067 + +0.3 50,068 - +0.3 50,068 - -0.3 50,068 - -0.5 50,068 - +0.7 50,068 - +0.4 50,068 - +0.5 50,558 + GFF3850 0.45 +0.0 50,558 + GFF3850 0.45 +0.2 50,558 + GFF3850 0.45 +1.0 50,558 + GFF3850 0.45 -0.5 50,558 + GFF3850 0.45 +0.4 50,558 + GFF3850 0.45 -1.4 50,558 + GFF3850 0.45 -0.1 50,558 + GFF3850 0.45 -0.6 50,558 + GFF3850 0.45 -0.3 50,558 + GFF3850 0.45 +0.4 50,558 + GFF3850 0.45 +0.4 50,559 - GFF3850 0.45 -1.0 50,559 - GFF3850 0.45 -1.3 50,559 - GFF3850 0.45 -1.3 50,559 - GFF3850 0.45 -0.8 50,559 - GFF3850 0.45 -0.1 50,559 - GFF3850 0.45 +0.7 50,559 - GFF3850 0.45 -0.4 50,573 + GFF3850 0.46 -0.1 50,573 + GFF3850 0.46 -0.9 50,573 + GFF3850 0.46 -0.5 50,573 + GFF3850 0.46 +0.1 50,573 + GFF3850 0.46 +1.2 50,573 + GFF3850 0.46 +1.5 50,574 - GFF3850 0.46 -0.4 50,574 - GFF3850 0.46 -1.2 50,574 - GFF3850 0.46 -0.9 50,574 - GFF3850 0.46 -0.8 50,574 - GFF3850 0.46 -0.6 50,574 - GFF3850 0.46 +0.1 50,574 - GFF3850 0.46 -0.8 50,609 + GFF3850 0.50 -0.7 50,609 + GFF3850 0.50 -0.8 50,609 + GFF3850 0.50 +0.0 50,609 + GFF3850 0.50 +0.7 50,610 - GFF3850 0.50 +0.8 50,610 - GFF3850 0.50 -0.1 50,610 - GFF3850 0.50 -0.5 50,610 - GFF3850 0.50 -0.3 50,612 + GFF3850 0.50 -0.1 50,612 + GFF3850 0.50 +0.7 50,612 + GFF3850 0.50 +0.1 50,612 + GFF3850 0.50 +0.0 50,612 + GFF3850 0.50 +0.7 50,612 + GFF3850 0.50 +0.7 50,612 + GFF3850 0.50 -0.5 50,612 + GFF3850 0.50 -0.8 50,612 + GFF3850 0.50 +0.6 50,612 + GFF3850 0.50 +0.5 50,612 + GFF3850 0.50 +0.1 50,612 + GFF3850 0.50 +0.9 50,612 + GFF3850 0.50 +0.4 50,612 + GFF3850 0.50 +0.2 50,612 + GFF3850 0.50 +0.7 50,612 + GFF3850 0.50 +1.5 50,612 + GFF3850 0.50 +0.0 50,612 + GFF3850 0.50 -0.7 50,612 + GFF3850 0.50 -0.5 50,613 - GFF3850 0.50 +0.3 50,613 - GFF3850 0.50 +1.3 50,613 - GFF3850 0.50 -1.0 50,613 - GFF3850 0.50 -1.3 50,613 - GFF3850 0.50 -1.1 50,613 - GFF3850 0.50 +0.8 50,613 - GFF3850 0.50 +0.8 50,613 - GFF3850 0.50 +1.1 50,613 - GFF3850 0.50 -0.6 50,613 - GFF3850 0.50 +0.5 50,613 - GFF3850 0.50 -0.9 50,613 - GFF3850 0.50 -0.9 50,613 - GFF3850 0.50 -0.2 50,613 - GFF3850 0.50 -0.5 50,613 - GFF3850 0.50 +0.6 50,613 - GFF3850 0.50 +0.1 50,613 - GFF3850 0.50 +0.9 50,613 - GFF3850 0.50 -0.1 50,639 + GFF3850 0.53 -0.3 50,639 + GFF3850 0.53 -0.8 50,639 + GFF3850 0.53 -0.3 50,639 + GFF3850 0.53 +0.3 50,639 + GFF3850 0.53 -0.3 50,639 + GFF3850 0.53 +0.0 50,639 + GFF3850 0.53 -0.5 50,639 + GFF3850 0.53 -1.8 50,639 + GFF3850 0.53 +0.9 50,639 + GFF3850 0.53 -0.5 50,640 - GFF3850 0.53 -1.0 50,640 - GFF3850 0.53 +0.6 50,640 - GFF3850 0.53 -0.3 50,640 - GFF3850 0.53 -0.5 50,640 - GFF3850 0.53 -0.4
Or see this region's nucleotide sequence