Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3824 and GFF3825 are separated by 131 nucleotides GFF3825 and GFF3826 overlap by 4 nucleotides GFF3826 and GFF3827 overlap by 4 nucleotides
GFF3824 - Argininosuccinate lyase (EC 4.3.2.1), at 13,262 to 14,659
GFF3824
GFF3825 - Autolysin sensor kinase (EC 2.7.3.-), at 14,791 to 15,789
GFF3825
GFF3826 - Response regulator receiver:LytTr DNA-binding region, at 15,786 to 16,541
GFF3826
GFF3827 - UPF0118 membrane protein SMc00793, at 16,538 to 17,818
GFF3827
Position (kb)
14
15
16 Strain fitness (log2 ratio)
-2
-1
0
1 at 14.681 kb on - strand at 14.681 kb on - strand at 14.701 kb on + strand at 14.702 kb on - strand at 14.702 kb on - strand at 14.702 kb on - strand at 14.702 kb on - strand at 15.026 kb on - strand, within GFF3825 at 15.026 kb on - strand, within GFF3825 at 15.064 kb on + strand, within GFF3825 at 15.064 kb on + strand, within GFF3825 at 15.064 kb on + strand, within GFF3825 at 15.064 kb on + strand, within GFF3825 at 15.065 kb on - strand, within GFF3825 at 15.065 kb on - strand, within GFF3825 at 15.406 kb on + strand, within GFF3825 at 15.714 kb on + strand at 15.715 kb on - strand at 15.715 kb on - strand at 15.715 kb on - strand at 15.715 kb on - strand at 16.099 kb on - strand, within GFF3826 at 16.260 kb on + strand, within GFF3826 at 16.260 kb on + strand, within GFF3826 at 16.260 kb on + strand, within GFF3826 at 16.261 kb on - strand, within GFF3826 at 16.261 kb on - strand, within GFF3826 at 16.275 kb on + strand, within GFF3826 at 16.275 kb on + strand, within GFF3826 at 16.281 kb on + strand, within GFF3826 at 16.281 kb on + strand, within GFF3826 at 16.281 kb on + strand, within GFF3826 at 16.281 kb on + strand, within GFF3826 at 16.281 kb on + strand, within GFF3826 at 16.282 kb on - strand, within GFF3826 at 16.282 kb on - strand, within GFF3826 at 16.282 kb on - strand, within GFF3826 at 16.282 kb on - strand, within GFF3826 at 16.282 kb on - strand, within GFF3826 at 16.282 kb on - strand, within GFF3826 at 16.422 kb on + strand, within GFF3826 at 16.423 kb on - strand, within GFF3826 at 16.423 kb on - strand, within GFF3826 at 16.423 kb on - strand, within GFF3826
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 14,681 - -0.3 14,681 - +0.1 14,701 + -1.8 14,702 - -0.1 14,702 - +0.1 14,702 - -1.9 14,702 - -0.2 15,026 - GFF3825 0.24 +1.2 15,026 - GFF3825 0.24 -1.9 15,064 + GFF3825 0.27 -0.9 15,064 + GFF3825 0.27 -1.2 15,064 + GFF3825 0.27 -0.1 15,064 + GFF3825 0.27 -1.1 15,065 - GFF3825 0.27 -1.4 15,065 - GFF3825 0.27 -0.6 15,406 + GFF3825 0.62 -0.4 15,714 + +1.5 15,715 - -0.6 15,715 - -0.9 15,715 - -1.9 15,715 - -0.9 16,099 - GFF3826 0.41 +1.5 16,260 + GFF3826 0.63 -0.6 16,260 + GFF3826 0.63 +0.5 16,260 + GFF3826 0.63 -0.5 16,261 - GFF3826 0.63 -1.3 16,261 - GFF3826 0.63 -0.9 16,275 + GFF3826 0.65 -1.1 16,275 + GFF3826 0.65 -0.7 16,281 + GFF3826 0.65 -1.3 16,281 + GFF3826 0.65 +0.7 16,281 + GFF3826 0.65 -1.0 16,281 + GFF3826 0.65 -0.3 16,281 + GFF3826 0.65 +0.3 16,282 - GFF3826 0.66 -0.9 16,282 - GFF3826 0.66 -2.4 16,282 - GFF3826 0.66 -1.3 16,282 - GFF3826 0.66 -0.6 16,282 - GFF3826 0.66 -0.6 16,282 - GFF3826 0.66 -2.0 16,422 + GFF3826 0.84 -1.3 16,423 - GFF3826 0.84 -0.4 16,423 - GFF3826 0.84 -0.3 16,423 - GFF3826 0.84 -0.5
Or see this region's nucleotide sequence