Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3778 and GFF3779 are separated by 164 nucleotides GFF3779 and GFF3780 are separated by 54 nucleotides GFF3780 and GFF3781 are separated by 25 nucleotides
GFF3778 - Wax ester synthase/acyl-CoA:diacylglycerol acyltransferase, at 270,284 to 271,984
GFF3778
GFF3779 - hypothetical protein, at 272,149 to 272,706
GFF3779
GFF3780 - hypothetical protein, at 272,761 to 273,234
GFF3780
GFF3781 - Transcriptional regulator, AcrR family, at 273,260 to 273,895
GFF3781
Position (kb) 272
273 Strain fitness (log2 ratio) -2
-1
0
1 at 271.321 kb on + strand, within GFF3778 at 271.496 kb on + strand, within GFF3778 at 271.497 kb on - strand, within GFF3778 at 271.497 kb on - strand, within GFF3778 at 271.523 kb on + strand, within GFF3778 at 271.523 kb on + strand, within GFF3778 at 271.523 kb on + strand, within GFF3778 at 271.523 kb on + strand, within GFF3778 at 271.524 kb on - strand, within GFF3778 at 271.524 kb on - strand, within GFF3778 at 271.524 kb on - strand, within GFF3778 at 271.524 kb on - strand, within GFF3778 at 271.524 kb on - strand, within GFF3778 at 271.580 kb on + strand, within GFF3778 at 271.580 kb on + strand, within GFF3778 at 271.580 kb on + strand, within GFF3778 at 271.580 kb on + strand, within GFF3778 at 271.580 kb on + strand, within GFF3778 at 271.581 kb on - strand, within GFF3778 at 271.581 kb on - strand, within GFF3778 at 271.581 kb on - strand, within GFF3778 at 271.581 kb on - strand, within GFF3778 at 271.581 kb on - strand, within GFF3778 at 271.581 kb on - strand, within GFF3778 at 271.652 kb on + strand, within GFF3778 at 271.652 kb on + strand, within GFF3778 at 271.652 kb on + strand, within GFF3778 at 271.653 kb on - strand, within GFF3778 at 271.653 kb on - strand, within GFF3778 at 271.653 kb on - strand, within GFF3778 at 271.653 kb on - strand, within GFF3778 at 271.683 kb on - strand, within GFF3778 at 271.852 kb on - strand at 272.032 kb on - strand at 272.032 kb on - strand at 272.048 kb on + strand at 272.063 kb on - strand at 272.081 kb on + strand at 272.081 kb on + strand at 272.081 kb on + strand at 272.081 kb on + strand at 272.082 kb on - strand at 272.082 kb on - strand at 272.082 kb on - strand at 272.082 kb on - strand at 272.082 kb on - strand at 272.200 kb on + strand at 272.201 kb on - strand at 272.496 kb on + strand, within GFF3779 at 272.497 kb on - strand, within GFF3779 at 273.392 kb on + strand, within GFF3781 at 273.392 kb on + strand, within GFF3781 at 273.392 kb on + strand, within GFF3781 at 273.393 kb on - strand, within GFF3781 at 273.393 kb on - strand, within GFF3781 at 273.393 kb on - strand, within GFF3781 at 273.542 kb on + strand, within GFF3781 at 273.542 kb on + strand, within GFF3781 at 273.554 kb on + strand, within GFF3781 at 273.555 kb on - strand, within GFF3781 at 273.555 kb on - strand, within GFF3781 at 273.555 kb on - strand, within GFF3781
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 271,321 + GFF3778 0.61 +1.2 271,496 + GFF3778 0.71 -0.4 271,497 - GFF3778 0.71 +0.0 271,497 - GFF3778 0.71 -0.1 271,523 + GFF3778 0.73 -0.8 271,523 + GFF3778 0.73 -0.1 271,523 + GFF3778 0.73 -0.1 271,523 + GFF3778 0.73 -2.3 271,524 - GFF3778 0.73 +1.4 271,524 - GFF3778 0.73 +0.4 271,524 - GFF3778 0.73 -1.0 271,524 - GFF3778 0.73 -0.6 271,524 - GFF3778 0.73 +0.1 271,580 + GFF3778 0.76 -0.3 271,580 + GFF3778 0.76 -0.8 271,580 + GFF3778 0.76 -0.1 271,580 + GFF3778 0.76 -2.0 271,580 + GFF3778 0.76 +0.2 271,581 - GFF3778 0.76 -0.4 271,581 - GFF3778 0.76 -0.7 271,581 - GFF3778 0.76 +1.4 271,581 - GFF3778 0.76 -1.7 271,581 - GFF3778 0.76 -0.2 271,581 - GFF3778 0.76 +1.3 271,652 + GFF3778 0.80 -0.9 271,652 + GFF3778 0.80 -0.3 271,652 + GFF3778 0.80 -0.5 271,653 - GFF3778 0.80 -2.3 271,653 - GFF3778 0.80 -0.0 271,653 - GFF3778 0.80 +0.2 271,653 - GFF3778 0.80 -1.2 271,683 - GFF3778 0.82 +0.8 271,852 - +1.0 272,032 - -0.6 272,032 - -0.8 272,048 + -0.4 272,063 - -0.5 272,081 + +0.2 272,081 + -0.8 272,081 + +1.1 272,081 + -0.1 272,082 - -1.4 272,082 - -0.8 272,082 - -0.3 272,082 - +0.3 272,082 - -2.0 272,200 + -0.3 272,201 - +0.5 272,496 + GFF3779 0.62 -1.1 272,497 - GFF3779 0.62 -0.6 273,392 + GFF3781 0.21 -0.2 273,392 + GFF3781 0.21 +0.7 273,392 + GFF3781 0.21 -0.4 273,393 - GFF3781 0.21 -1.1 273,393 - GFF3781 0.21 -0.3 273,393 - GFF3781 0.21 -0.5 273,542 + GFF3781 0.44 -0.3 273,542 + GFF3781 0.44 -1.8 273,554 + GFF3781 0.46 -0.4 273,555 - GFF3781 0.46 -0.0 273,555 - GFF3781 0.46 -0.2 273,555 - GFF3781 0.46 -0.1
Or see this region's nucleotide sequence