Strain Fitness in Variovorax sp. SCN45 around GFF36

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF34 and GFF35 overlap by 4 nucleotidesGFF35 and GFF36 are separated by 21 nucleotidesGFF36 and GFF37 overlap by 4 nucleotidesGFF37 and GFF38 are separated by 12 nucleotides GFF34 - Uncharacterized metal-dependent hydrolase YcfH, at 37,616 to 38,422 GFF34 GFF35 - FOG: Ankyrin repeat, at 38,419 to 39,099 GFF35 GFF36 - Similar to Agmatine deiminase, at 39,121 to 40,245 GFF36 GFF37 - Similar to Agmatine deiminase, at 40,242 to 41,195 GFF37 GFF38 - no description, at 41,208 to 41,465 GFF38 Position (kb) 39 40 41Strain fitness (log2 ratio) -2 -1 0 1 2at 38.159 kb on + strand, within GFF34at 38.159 kb on + strand, within GFF34at 38.159 kb on + strand, within GFF34at 38.159 kb on + strand, within GFF34at 38.159 kb on + strand, within GFF34at 38.159 kb on + strand, within GFF34at 38.159 kb on + strand, within GFF34at 38.160 kb on - strand, within GFF34at 38.160 kb on - strand, within GFF34at 38.160 kb on - strand, within GFF34at 38.160 kb on - strand, within GFF34at 38.160 kb on - strand, within GFF34at 38.160 kb on - strand, within GFF34at 38.264 kb on + strand, within GFF34at 38.264 kb on + strand, within GFF34at 38.309 kb on + strand, within GFF34at 38.309 kb on + strand, within GFF34at 38.309 kb on + strand, within GFF34at 38.309 kb on + strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.310 kb on - strand, within GFF34at 38.318 kb on + strand, within GFF34at 38.318 kb on + strand, within GFF34at 38.318 kb on + strand, within GFF34at 38.319 kb on - strand, within GFF34at 38.319 kb on - strand, within GFF34at 38.319 kb on - strand, within GFF34at 38.319 kb on - strand, within GFF34at 38.319 kb on - strand, within GFF34at 38.446 kb on + strandat 38.797 kb on + strand, within GFF35at 38.797 kb on + strand, within GFF35at 38.797 kb on + strand, within GFF35at 38.797 kb on + strand, within GFF35at 38.797 kb on + strand, within GFF35at 38.798 kb on - strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.908 kb on + strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 38.909 kb on - strand, within GFF35at 39.097 kb on + strandat 39.097 kb on + strandat 39.117 kb on + strandat 39.117 kb on + strandat 39.117 kb on + strandat 39.118 kb on - strandat 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.296 kb on + strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.297 kb on - strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.308 kb on + strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.309 kb on - strand, within GFF36at 39.449 kb on + strand, within GFF36at 39.449 kb on + strand, within GFF36at 39.449 kb on + strand, within GFF36at 39.450 kb on - strand, within GFF36at 39.450 kb on - strand, within GFF36at 39.450 kb on - strand, within GFF36at 39.450 kb on - strand, within GFF36at 39.509 kb on + strand, within GFF36at 39.509 kb on + strand, within GFF36at 39.755 kb on + strand, within GFF36at 39.755 kb on + strand, within GFF36at 39.756 kb on - strand, within GFF36at 39.756 kb on - strand, within GFF36at 40.429 kb on + strand, within GFF37at 40.429 kb on + strand, within GFF37at 40.430 kb on - strand, within GFF37at 40.430 kb on - strand, within GFF37at 40.430 kb on - strand, within GFF37at 40.630 kb on + strand, within GFF37at 40.889 kb on + strand, within GFF37at 40.890 kb on - strand, within GFF37at 41.102 kb on - strandat 41.102 kb on - strandat 41.169 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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38,159 + GFF34 0.67 -0.8
38,159 + GFF34 0.67 +1.2
38,159 + GFF34 0.67 -0.1
38,159 + GFF34 0.67 +0.0
38,159 + GFF34 0.67 -0.2
38,159 + GFF34 0.67 -0.8
38,159 + GFF34 0.67 +0.6
38,160 - GFF34 0.67 +1.2
38,160 - GFF34 0.67 -0.3
38,160 - GFF34 0.67 -1.0
38,160 - GFF34 0.67 +0.1
38,160 - GFF34 0.67 -0.1
38,160 - GFF34 0.67 -0.0
38,264 + GFF34 0.80 +0.4
38,264 + GFF34 0.80 +0.8
38,309 + GFF34 0.86 -0.2
38,309 + GFF34 0.86 -0.0
38,309 + GFF34 0.86 +0.1
38,309 + GFF34 0.86 -1.9
38,310 - GFF34 0.86 -0.5
38,310 - GFF34 0.86 -0.8
38,310 - GFF34 0.86 -0.1
38,310 - GFF34 0.86 -0.0
38,310 - GFF34 0.86 -0.1
38,310 - GFF34 0.86 +0.6
38,310 - GFF34 0.86 +1.5
38,310 - GFF34 0.86 +0.3
38,318 + GFF34 0.87 -1.2
38,318 + GFF34 0.87 -1.3
38,318 + GFF34 0.87 -1.3
38,319 - GFF34 0.87 -0.0
38,319 - GFF34 0.87 -0.6
38,319 - GFF34 0.87 -1.1
38,319 - GFF34 0.87 -1.7
38,319 - GFF34 0.87 +0.1
38,446 + -0.2
38,797 + GFF35 0.56 +0.4
38,797 + GFF35 0.56 -0.5
38,797 + GFF35 0.56 +0.2
38,797 + GFF35 0.56 +0.2
38,797 + GFF35 0.56 +0.2
38,798 - GFF35 0.56 -0.6
38,908 + GFF35 0.72 -1.0
38,908 + GFF35 0.72 -0.1
38,908 + GFF35 0.72 +0.4
38,908 + GFF35 0.72 +0.4
38,908 + GFF35 0.72 -0.4
38,908 + GFF35 0.72 -0.5
38,908 + GFF35 0.72 -0.6
38,908 + GFF35 0.72 -0.3
38,908 + GFF35 0.72 -1.0
38,908 + GFF35 0.72 -0.1
38,908 + GFF35 0.72 -0.5
38,908 + GFF35 0.72 -1.1
38,908 + GFF35 0.72 +0.1
38,908 + GFF35 0.72 +0.6
38,908 + GFF35 0.72 -0.2
38,908 + GFF35 0.72 -0.2
38,908 + GFF35 0.72 -0.5
38,908 + GFF35 0.72 +0.5
38,908 + GFF35 0.72 -1.1
38,908 + GFF35 0.72 +0.6
38,908 + GFF35 0.72 -1.1
38,909 - GFF35 0.72 -0.2
38,909 - GFF35 0.72 +0.2
38,909 - GFF35 0.72 +0.2
38,909 - GFF35 0.72 +0.2
38,909 - GFF35 0.72 +0.5
38,909 - GFF35 0.72 -0.2
38,909 - GFF35 0.72 -0.6
38,909 - GFF35 0.72 +0.2
38,909 - GFF35 0.72 -0.1
38,909 - GFF35 0.72 -0.3
38,909 - GFF35 0.72 +0.2
38,909 - GFF35 0.72 -0.3
38,909 - GFF35 0.72 -0.6
38,909 - GFF35 0.72 +0.8
38,909 - GFF35 0.72 -0.6
38,909 - GFF35 0.72 +0.1
38,909 - GFF35 0.72 -0.4
38,909 - GFF35 0.72 -0.7
38,909 - GFF35 0.72 -1.7
38,909 - GFF35 0.72 -0.4
38,909 - GFF35 0.72 -2.4
38,909 - GFF35 0.72 -0.3
38,909 - GFF35 0.72 -0.1
39,097 + -0.5
39,097 + -0.5
39,117 + -0.2
39,117 + -0.4
39,117 + +0.8
39,118 - -0.3
39,296 + GFF36 0.16 -1.4
39,296 + GFF36 0.16 +0.4
39,296 + GFF36 0.16 -0.0
39,296 + GFF36 0.16 +0.6
39,296 + GFF36 0.16 -0.3
39,296 + GFF36 0.16 +0.3
39,296 + GFF36 0.16 -0.0
39,296 + GFF36 0.16 +0.8
39,296 + GFF36 0.16 +0.2
39,296 + GFF36 0.16 -0.8
39,297 - GFF36 0.16 -0.3
39,297 - GFF36 0.16 -0.7
39,297 - GFF36 0.16 +2.2
39,297 - GFF36 0.16 -0.3
39,297 - GFF36 0.16 +0.3
39,297 - GFF36 0.16 -1.7
39,297 - GFF36 0.16 +0.1
39,297 - GFF36 0.16 -0.1
39,297 - GFF36 0.16 +1.2
39,297 - GFF36 0.16 +0.2
39,297 - GFF36 0.16 +0.4
39,297 - GFF36 0.16 +1.6
39,308 + GFF36 0.17 -0.9
39,308 + GFF36 0.17 -0.2
39,308 + GFF36 0.17 +0.2
39,308 + GFF36 0.17 +0.6
39,308 + GFF36 0.17 -1.9
39,308 + GFF36 0.17 +0.5
39,308 + GFF36 0.17 -0.1
39,308 + GFF36 0.17 -0.5
39,309 - GFF36 0.17 -0.2
39,309 - GFF36 0.17 +0.4
39,309 - GFF36 0.17 -0.5
39,309 - GFF36 0.17 -0.8
39,309 - GFF36 0.17 +0.9
39,309 - GFF36 0.17 +0.2
39,309 - GFF36 0.17 +0.4
39,449 + GFF36 0.29 -0.8
39,449 + GFF36 0.29 -0.4
39,449 + GFF36 0.29 -0.3
39,450 - GFF36 0.29 -0.1
39,450 - GFF36 0.29 +0.5
39,450 - GFF36 0.29 -0.4
39,450 - GFF36 0.29 -0.2
39,509 + GFF36 0.34 -0.5
39,509 + GFF36 0.34 -0.4
39,755 + GFF36 0.56 -0.8
39,755 + GFF36 0.56 -0.2
39,756 - GFF36 0.56 -1.9
39,756 - GFF36 0.56 +0.2
40,429 + GFF37 0.20 -0.9
40,429 + GFF37 0.20 -1.2
40,430 - GFF37 0.20 +0.2
40,430 - GFF37 0.20 -0.8
40,430 - GFF37 0.20 -1.1
40,630 + GFF37 0.41 -0.2
40,889 + GFF37 0.68 -0.5
40,890 - GFF37 0.68 -0.2
41,102 - +1.0
41,102 - -1.0
41,169 + -0.8

Or see this region's nucleotide sequence