Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3589 and GFF3590 are separated by 28 nucleotides GFF3590 and GFF3591 are separated by 0 nucleotides
GFF3589 - Transcriptional regulator, LysR family, at 100,250 to 101,164
GFF3589
GFF3590 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 101,193 to 102,107
GFF3590
GFF3591 - Glutathione reductase (EC 1.8.1.7), at 102,108 to 103,466
GFF3591
Position (kb) 101
102
103 Strain fitness (log2 ratio) -1
0
1
2
3 at 100.454 kb on + strand, within GFF3589 at 100.454 kb on + strand, within GFF3589 at 100.454 kb on + strand, within GFF3589 at 100.455 kb on - strand, within GFF3589 at 100.691 kb on + strand, within GFF3589 at 100.727 kb on + strand, within GFF3589 at 100.727 kb on + strand, within GFF3589 at 100.727 kb on + strand, within GFF3589 at 100.727 kb on + strand, within GFF3589 at 100.728 kb on - strand, within GFF3589 at 100.728 kb on - strand, within GFF3589 at 100.728 kb on - strand, within GFF3589 at 100.922 kb on + strand, within GFF3589 at 100.922 kb on + strand, within GFF3589 at 100.923 kb on - strand, within GFF3589 at 100.923 kb on - strand, within GFF3589 at 101.057 kb on + strand, within GFF3589 at 101.057 kb on + strand, within GFF3589 at 101.057 kb on + strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.058 kb on - strand, within GFF3589 at 101.232 kb on + strand at 101.232 kb on + strand at 101.232 kb on + strand at 101.232 kb on + strand at 101.232 kb on + strand at 101.233 kb on - strand at 101.233 kb on - strand at 101.235 kb on + strand at 101.235 kb on + strand at 101.568 kb on + strand, within GFF3590 at 101.568 kb on + strand, within GFF3590 at 101.568 kb on + strand, within GFF3590 at 101.568 kb on + strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.569 kb on - strand, within GFF3590 at 101.751 kb on + strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.752 kb on - strand, within GFF3590 at 101.753 kb on + strand, within GFF3590 at 101.934 kb on + strand, within GFF3590 at 101.934 kb on + strand, within GFF3590 at 101.935 kb on - strand, within GFF3590 at 102.409 kb on + strand, within GFF3591 at 102.409 kb on + strand, within GFF3591 at 102.463 kb on + strand, within GFF3591 at 102.571 kb on + strand, within GFF3591 at 102.571 kb on + strand, within GFF3591 at 102.572 kb on - strand, within GFF3591 at 102.572 kb on - strand, within GFF3591 at 102.935 kb on - strand, within GFF3591
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 100,454 + GFF3589 0.22 -0.9 100,454 + GFF3589 0.22 -1.3 100,454 + GFF3589 0.22 -0.0 100,455 - GFF3589 0.22 -0.2 100,691 + GFF3589 0.48 -0.8 100,727 + GFF3589 0.52 +0.1 100,727 + GFF3589 0.52 -0.3 100,727 + GFF3589 0.52 -0.6 100,727 + GFF3589 0.52 +0.6 100,728 - GFF3589 0.52 +0.7 100,728 - GFF3589 0.52 -0.1 100,728 - GFF3589 0.52 -0.1 100,922 + GFF3589 0.73 -0.1 100,922 + GFF3589 0.73 +3.0 100,923 - GFF3589 0.74 -1.0 100,923 - GFF3589 0.74 +0.0 101,057 + GFF3589 0.88 +0.2 101,057 + GFF3589 0.88 +0.5 101,057 + GFF3589 0.88 +0.9 101,058 - GFF3589 0.88 +0.9 101,058 - GFF3589 0.88 -0.3 101,058 - GFF3589 0.88 -0.5 101,058 - GFF3589 0.88 +0.1 101,058 - GFF3589 0.88 +0.7 101,058 - GFF3589 0.88 +0.6 101,058 - GFF3589 0.88 -0.6 101,058 - GFF3589 0.88 -0.6 101,232 + -0.6 101,232 + +0.5 101,232 + -1.3 101,232 + -0.5 101,232 + -0.2 101,233 - -0.1 101,233 - -1.4 101,235 + -1.3 101,235 + -0.7 101,568 + GFF3590 0.41 -0.4 101,568 + GFF3590 0.41 +1.3 101,568 + GFF3590 0.41 -0.7 101,568 + GFF3590 0.41 -1.0 101,569 - GFF3590 0.41 -1.1 101,569 - GFF3590 0.41 -0.6 101,569 - GFF3590 0.41 +0.0 101,569 - GFF3590 0.41 +0.7 101,751 + GFF3590 0.61 -1.0 101,752 - GFF3590 0.61 -0.3 101,752 - GFF3590 0.61 -1.2 101,752 - GFF3590 0.61 +1.1 101,752 - GFF3590 0.61 -1.3 101,752 - GFF3590 0.61 -0.3 101,752 - GFF3590 0.61 -0.7 101,753 + GFF3590 0.61 -0.7 101,934 + GFF3590 0.81 -0.3 101,934 + GFF3590 0.81 -1.3 101,935 - GFF3590 0.81 -0.2 102,409 + GFF3591 0.22 -0.7 102,409 + GFF3591 0.22 -1.1 102,463 + GFF3591 0.26 -0.3 102,571 + GFF3591 0.34 -0.1 102,571 + GFF3591 0.34 -0.2 102,572 - GFF3591 0.34 +0.2 102,572 - GFF3591 0.34 -0.2 102,935 - GFF3591 0.61 -0.4
Or see this region's nucleotide sequence