Strain Fitness in Variovorax sp. SCN45 around GFF3574

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
85,480	+		GFF3573	0.14	-0.5
85,481	-		GFF3573	0.14	-1.0
85,570	+		GFF3573	0.23	-0.3
85,570	+		GFF3573	0.23	-0.9
85,570	+		GFF3573	0.23	-1.3
85,571	-		GFF3573	0.23	-0.6
85,963	+		GFF3573	0.65	-0.6
85,972	+		GFF3573	0.66	-1.6
85,972	+		GFF3573	0.66	-0.4
86,234	-				+0.4
86,670	+		GFF3574	0.33	-1.3
86,670	+		GFF3574	0.33	+1.0
86,670	+		GFF3574	0.33	+0.7
86,677	-		GFF3574	0.34	-0.5
86,826	+		GFF3574	0.52	-0.1
86,826	+		GFF3574	0.52	+0.7
86,827	-		GFF3574	0.52	+2.0
86,827	-		GFF3574	0.52	+0.4
86,827	-		GFF3574	0.52	-0.1
86,827	-		GFF3574	0.52	+0.0
86,827	-		GFF3574	0.52	+1.2
86,847	+		GFF3574	0.54	-0.8
86,848	-		GFF3574	0.54	-0.8
86,848	-		GFF3574	0.54	+1.2
86,848	-		GFF3574	0.54	+0.7
86,848	-		GFF3574	0.54	-0.7
86,928	+		GFF3574	0.64	-0.3
86,928	+		GFF3574	0.64	+0.6
86,929	-		GFF3574	0.64	-0.4
87,422	+		GFF3575	0.28	-0.8
87,422	+		GFF3575	0.28	-0.3
87,722	+		GFF3575	0.72	-0.1
87,722	+		GFF3575	0.72	+1.5
87,722	+		GFF3575	0.72	-0.2
87,723	-		GFF3575	0.72	-0.4
87,723	-		GFF3575	0.72	-1.3
87,723	-		GFF3575	0.72	+0.2
88,074	+		GFF3576	0.25	+0.0
88,074	+		GFF3576	0.25	-0.7
88,075	-		GFF3576	0.25	+0.5