Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3416 and GFF3417 overlap by 4 nucleotides GFF3417 and GFF3418 overlap by 8 nucleotides GFF3418 and GFF3419 are separated by 74 nucleotides
GFF3416 - Alkanesulfonates transport system permease protein, at 240,183 to 240,962
GFF3416
GFF3417 - Alkanesulfonates transport system permease protein, at 240,959 to 241,729
GFF3417
GFF3418 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 241,722 to 242,501
GFF3418
GFF3419 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 242,576 to 243,535
GFF3419
Position (kb) 241
242
243 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 240.836 kb on + strand, within GFF3416 at 240.946 kb on + strand at 241.653 kb on + strand at 241.654 kb on - strand at 241.670 kb on + strand at 241.775 kb on - strand at 241.900 kb on + strand, within GFF3418 at 241.900 kb on + strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.901 kb on - strand, within GFF3418 at 241.940 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.334 kb on + strand, within GFF3418 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.730 kb on + strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.731 kb on - strand, within GFF3419 at 242.905 kb on - strand, within GFF3419 at 243.042 kb on + strand, within GFF3419 at 243.117 kb on + strand, within GFF3419 at 243.369 kb on + strand, within GFF3419 at 243.369 kb on + strand, within GFF3419 at 243.370 kb on - strand, within GFF3419 at 243.370 kb on - strand, within GFF3419
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 240,836 + GFF3416 0.84 +2.4 240,946 + +0.3 241,653 + -1.9 241,654 - -0.2 241,670 + -0.1 241,775 - -0.5 241,900 + GFF3418 0.23 -1.3 241,900 + GFF3418 0.23 -0.8 241,901 - GFF3418 0.23 +0.8 241,901 - GFF3418 0.23 -0.6 241,940 + GFF3418 0.28 -0.7 242,334 + GFF3418 0.78 -0.7 242,334 + GFF3418 0.78 -1.3 242,730 + GFF3419 0.16 -1.1 242,730 + GFF3419 0.16 -0.2 242,730 + GFF3419 0.16 -0.5 242,730 + GFF3419 0.16 +2.8 242,730 + GFF3419 0.16 -0.5 242,731 - GFF3419 0.16 -1.8 242,731 - GFF3419 0.16 -0.3 242,731 - GFF3419 0.16 -0.2 242,905 - GFF3419 0.34 -0.4 243,042 + GFF3419 0.49 +0.3 243,117 + GFF3419 0.56 -0.2 243,369 + GFF3419 0.83 -0.2 243,369 + GFF3419 0.83 -1.2 243,370 - GFF3419 0.83 +0.6 243,370 - GFF3419 0.83 +1.4
Or see this region's nucleotide sequence